• 제목/요약/키워드: "L-Skin Care"

검색결과 89건 처리시간 0.024초

쥐참외뿌리 추출물의 항산화, 미백, 항염증 활성 연구 (A Study on the Antioxidant, Whitening and Anti-Inflammatory Activities of Trichosanthis Cucumeroidis Radix Extract)

  • 유선희;이은주
    • 한국응용과학기술학회지
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    • 제39권4호
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    • pp.580-587
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    • 2022
  • 본 연구에서는 쥐참외뿌리 추출물의 항산화 활성 및 미백, 항염증 활성에 대한 생리활성 효과와 기능성 소재로써의 활용 가능성을 확인해 보고자 하였다. 쥐참외뿌리 추출물의 DPPH 라디칼 소거 활성을 통한 항산화 활성, 멜라닌 세포인 B16F10 melanoma 세포에 대한 멜라닌 생성 억제능을 통한 미백 활성 효과, 대식 세포인 RAW 264.7 세포에 대한 NO 생성 억제능을 통한 항염증 활성 효과를 확인해 보고자 하였다. 연구결과, 쥐참외뿌리 추출물은 농도 의존적으로 DPPH 라디칼 소거활성이 확인되었으며, 양성 대조군인 Ascorbic acid와 비슷한 DPPH 라디컬 소거활성이 확인되었다. 100 nM 𝛼-MSH로 유도된 멜라닌 생성 억제 활성과 LPS 1 ㎍/mL로 유도된 NO 생성 억제능을 유의하게 억제 하는 것을 확인되었다. 이에 따라 쥐참외뿌리 추출물은 항산화 및 미백, 항염증 효과를 가진 기능성 소재로써의 활용 가능성이 있음이 사료되어 진다.

Efficacy and Safety of Sublingual Immunotherapy in Elderly Rhinitis Patients Sensitized to House Dust Mites

  • Kim, Ji Hye;Lee, Ji Ho;Ye, Young-Min;Lee, Jae-Hyun;Park, Jung Won;Hur, Gyu-Young;Kim, Joo-Hee;Lee, Hyn-Young;Shin, Yoo Seob;Yang, Eun-Mi;Park, Hae-Sim
    • Allergy, Asthma & Immunology Research
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    • 제10권6호
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    • pp.675-685
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    • 2018
  • Purpose: This study aims to determine the efficacy and safety of house dust mite (HDM)-sublingual immunotherapy (SLIT) in elderly patients with AR. Methods: A total of 45 patients aged ${\geq}60years$ with HDM-induced AR who had ${\geq}3$ A/H ratio on skin prick test and/or ${\geq}0.35IU/L$ to both Dermatophagoides farinae and Dermatophagoides pteronyssinus by ImmunoCAP were enrolled in 4 university hospitals. To evaluate additional effects of HDM-SLIT, they were randomized to the SLIT-treated group (n = 30) or control group (n = 15). Rhinoconjunctivitis total symptom score (RTSS), rhinoscopy score, Korean rhinoconjunctivitis quality of life questionnaire, rhinitis control assessment test, asthma control test scores, and adverse reactions, were assessed at the first visit (V1) and after 1 year of treatment (V5); for immunological evaluation, serum levels of HDM-specific immunoglobulin A/IgE/IgG1/IgG4 antibodies and basophil response to HDMs were compared between V1 and V5 in both groups. Results: There were no significant differences in demographics, RTSS, skin reactivity to HDMs, or serum total/specific IgE levels to HDMs (P > 0.05, respectively) between the 2 groups. Nasal symptom score and RTSS decreased significantly at year 1 in the 2 groups (P < 0.05). There were no significant differences in percent decrease in nasal symptom score and RTSS at year 1 between the 2 groups (P > 0.05); however, rhinoscopic nasal symptom score decreased significantly in the SLIT-treated group (P < 0.05). Immunological studies showed that serum specific IgA levels (not specific IgE/IgG) and CD203c expression on basophils decreased significantly at V5 in the SLIT-treated group (P = 0.011 and P = 0.001, respectively), not in the control group. The control group required more medications compared to the treatment group, but there were no differences in adverse reactions. Conclusions: It is suggested that HDM-SLIT for 1 year could induce symptom improvement and may induce immunomodulation in elderly rhinitis patients.

연잎 에탄올 추출물의 항산화 효과 및 아질산염 소거능 (Antioxidative Activity and Nitrite Scavenging Ability of Ethanol Extract from Nelumbo nucifera Leaves)

  • 임진아;이은숙;백승화
    • 동의생리병리학회지
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    • 제22권3호
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    • pp.654-659
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    • 2008
  • To confirm possibility of Nelumbo nucifera leaves as biofunctional material, we investigated the antioxidant activity and nitrite scavenging ability of ethanol extract from Nelumbo nucifera leaves. Nelumbo nucifera leaves were extracted with ethanol and concentrated under vacuum using rotary evaporator. Then, antioxidative activity and nitrite scavenging ability of the extract were examined in vitro. Electron -donating ability of the extract at RC50 was 90.19 ${\mu}g/mL$. After addition of 0.96 mg/mL, autooxidation of pyrogallol decreased to 66.19% by superoxide dismutase-like activity. In antioxidative activity of the extract against linoleic acid during incubation times of 24, 48, and 96 hours at $40^{\circ}C$, lipid peroxidation values significantly decreased to 72.53%, 82.00%, 84.69% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and the value revealed to be $282.84\;{\pm}\;9.03$ GAE ${\mu}g/mg$ of the extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 45.55% by addition of 0.2 mg/mL. These results suggest that ethanol extract from N. nucifera leaves can be used as bioactive and functional material.

간호학생의 임상실습 간호활동시간 분석 (Analysis of Nursing Care Activities of Nursing Students in Clinical Experience)

  • 이정희;성영희;정연이;김정숙
    • 한국간호교육학회지
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    • 제4권2호
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    • pp.249-263
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    • 1998
  • The competence of newly graduated nurses is based on various clinical expriences gained when they were students. Therefore, instructors of nursing students, professors in nursing schools or directors in hospitals must play a critical role in assisting them to obtain various knowledge and experienced nursing skills. The purpose of this study was to investigate nursing care activities and nursing care hours practiced by nursing students in a general hospital. The subject students were total 214 nursing students, 2nd graders(sophomores) and 3rd graders(juniors) from 5 Junior Nursing Colleges in Seoul and they practiced at S general hospital to gain clinical experience. The data were collected for 4 days. The tools for this study were the direct nursing care activity list consisted of 15 nursing areas and the indirect nursing care activity list consisted of 9 nursing areas. The subject students were supposed to record their own score. The results of this study are ; 1. The nursing care hours per nursing student 1) The average total nursing care hours a day per each nursing student are 362.65 mins(6.04hr), the direct nursing care hours per each nursing stuent are 202.09 mins(direct nursing care rate 56.0%) and it is higher than the indirect nursing care hours, 159.75mins(indirect nursing care rate 44.0%). The direct nursing care rate of each student by a team approach in the evening shift(56.3%) is higher than that in the day shift(55.8%). 2) The hours of checking vital signs are the longest(47.35mins) among the direct nursing care activites and next is in order of counseling 8l emotional support, nurse rounds, and accompaning a patient during examination. The hours of reporting are the longest(32.39mins) among the indirect nursing care activites, and next is the activities related to education such as reviewing chart, looking up references, etc. 3) The freqency of checking vital signs practiced by nursing student is the highest(the average of 55.7 times) among the direct nursing care activities and next is in order of nurse rounds, assistance of feeding, and counseling & emotional support. The required time for nursing students to accompany their patient during examination is the longest(20.7mins) and next are in order of restriction on patient' activity, orientated by a head nurse, skin care, sitz bath, bathing & hair shampoo, and assisting with patients' exercise. 2. The nursing care hours per grader 1) The average hours of total nursing care per a nursing student are 369.2mins(6.2hrs) to 2nd graders, 355.9mins(5.9 hrs) to third graders. The direct nursing care rate per each nursing student to 3rd graders(59.3%) was higher than that to End graders(52.8%). 2) For 2nd graders, the highly marked nursing activities are teaching associated with direct nursing care activities such as drawing up papers, looking up references, reviewing charts, and being orientated by staff nurses. For 3rd graders, measurments, observations, and nurse rounds in indirect nursing care activities are taken highly 3) The most frequent practice of the nursing care activites is checking vital signs : 65 times to 3rd graders and 46.5 times to 2nd graders. Our suggestions based on the results of this study are : 1. It is recommanded to repeat the same designed study in a variety of clinical fields for further study. 2. It is recommanded to collect data not by self-record method but by observated method. 3. It is needed for nursing instructors in Nursing Schools and in hospitals to develop the guidelines and check-list of clinical practice courses.

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부처손 잎 추출물의 항산화, 항염 및 피부재생 효능 (Anti-oxidant, Anti-inflammatory, and Wound Healing Activities of Selaginella tamariscina Leaf Extract)

  • 김유진;이지안
    • 융합정보논문지
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    • 제11권4호
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    • pp.194-202
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    • 2021
  • 부처손 잎은 전통한방에서 탈항, 혈뇨, 부정출혈, 월경불순, 자궁출혈 및 만성간염을 치료 목적으로 사용되어 왔다. 본 연구에서는 부처손을 이용하여 얻은 열수추출물(STW)과 80%에탄올추출물(STE)의 항산화, 항염 및 세포 재생활성을 평가하였다. 그 결과 열수추출물과 에탄올추출물의 폴리페놀 화합물 함량은 각각 38.108±0.766 mg/g, 17.927±1.064 mg/g이었다. DPPH와 ABTS radical 소거능 IC50은 열수추출물이 에탄올추출물에 비해 2배 이상 낮았다. MTT 결과, RAW264.7 세포 생존율은 두 추출물 모두 1 mg/mL에서 6% 감소한 반면, HaCaT 세포의 생존율은 50 ㎍/mL에서 18%로 증가하였다. 열수추출물과 에탄올추출물은 LPS로 자극한 RAW264.7 세포의 NO, TNF-�� 그리고 COX-2, PGE2 등의 염증 매개물질의 생성을 억제하였다. 또한 TNF-a에 의한 각질형성 세포의 손상을 저해시켰다. 이상의 결과로 부처손 열수추출물과 에탄올추출물은 피부건강과 미용 식품을 위한 기능성 원료로 가능성이 높다고 사료된다.

퇴원환자의 가정간호요구와 가정간호사업의 효과 분석 - 일 종합병원을 중심으로 (A Study of Home Care Needs of Patients at Discharge and Effects of Home Care -Centered on Patients Discharged from a Rural General Hospilal-)

  • 최연순;김대현;서미혜;김조자;강규숙
    • 대한간호
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    • 제31권4호
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    • pp.77-99
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    • 1992
  • The study was carried out at W. hospital, an affiliated hospital of Y university, involved a total of 163 patients who were discharged from the hospital between May 1990 und March 199J. Data collection was twice, just prior to discharge and a minimum of three months post discharge. Thirty patients who lived within a hour travel time of the hospital received home care during the three months post discharge. Nursing diagnoses and nursing interventions For these patients were analyzed in this study. The results of the study are summarized as follows : 1. Discharge needs for the subjects of the study were analyzed using Gordon's eleven Functional categories and it was found that 48.3% of the total sample had identified nursing needs. Of these, the needs most frequently identified were in the categories of sexuality, 79.3 %, health perception, 68.2 % self concept, 62.5 %, and sleep and rest 62.5 %. Looking ut j he nursing diagnosis that were made for the 30 patients receiving home care, the following diagnoses were the most frequently given; alteration in sexual pattern 79.3%, alterations in health maintenance, 72.6%, alteration in comfort, 68.0%, depression, 64.0%, noncompliance with diet therapy, 6.3.7%, alteration in self concept, 55.6%, and alteration in sleep pattern, 53%. 2. In looking at the effects of home nursing care as demonstrated by changes in the functional categories over the three month period, it was Found that of the 11 functional categories, the need level for health perception, nutrition, activity and self concept decreased slightly over the three month period. On the average sleep patterns improved, but restfulness was slightly less and bowel elimination patterns improved but satisfaction with urinary elimination was slightly less. On the other hand, role enactment, sexuality, stress management and spirituality decreased slightly. The only results that were statistically significant at the 0.05 level were improvement. in digestion and decrease in pain. No statistically significant changes were found in ability related to ADL, the total ADL Score at discharge was $19.78{\pm}8.234, and after 3 months $19.01{\pm}8.12$. Considering that a majority of the patients were over 60 years of age and that many had brain or spinal cord injuries, the fact that their ADL ability did nor deteriorate after discharge can be interpreted as related to a positive impact by the home health care nurses. Similarly there was a slight be not statistically significant decrease in the quality of life scores between the two lest times(l47.83 at discharge and 113.02 at the three month period). Again, when the chronic nature of thee problems facing these patients is considered this maintenance of quality of life can be interpreted as a positive impact by the home health care nurses. 3. One of the home care nursing activities was diagnosis. For this activity it was found that for nine functional health categories(sexuality and spirituality excepted) there were 20 nursing diagnoses. The most frequent were noncompliance, alteration in skin integrity both actual and potential, and impaired physical mobility in that order. 4. Delivery of home health care by the home health nurses included the following nursing activities; assessment, patient education, demonstration of care activities, counselling, direct care to the patient and referrals. Direct care included changing dressings, bladder irrigations, changing Foley catheters, measurement of residual urine, perineal care, position change, back care, oral hygiene, exercise and massage of motion exercises, cleansing enemas, tracheostomy suctioning and tracheostomy care, care of dentures, applications of heat and other similar nursing activities. In conclusion almost 50% of (he sample indicated a need for continued nursing care at the time of discharge and for the patients in the sample who received home care there was a slight decrease in nursing needs but while the patients had chronic and debilitation problems there was ill decrease in ADL abilities or in quality of life. Further study needs Lo be done La increase the reliability and validity of the tool that was used to measure home health care needs. It is also recommended that study by done using a randomized sampling with a control group to compare patients who receive home care with those who do not.

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Preparation and Antioxidant Activities In Vitro of a Designed Antioxidant Peptide from Pinctada fucata by Recombinant Escherichia coli

  • Wu, Yanyan;Ma, Yongkai;Li, Laihao;Yang, Xianqing
    • Journal of Microbiology and Biotechnology
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    • 제28권1호
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    • pp.1-11
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    • 2018
  • An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structure-activity relationship in these peptides. To prepare antioxidant peptides better and improve their stability, the design and expression of an antioxidant peptide from Pinctada fucata (named DSAOP) was studied. The peptide contains a common precursor of an expression vector containing an ${\alpha}$-helix tandemly linked according to the BamHI restriction sites. The DNA fragments encoding DSAOP were synthesized and subcloned into the expression vector pET-30a (+), and the peptide was expressed mostly as soluble protein in recombinant Escherichia coli. Meanwhile, the DPPH radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging activity of DSAOP $IC_{50}$ values were $0.136{\pm}0.006$, $0.625{\pm}0.025$, and $0.306{\pm}0.015mg/ml$, respectively, with 2-fold higher DPPH radical scavenging activity compared with chemosynthesized AOP (p < 0.05), as well as higher superoxide radical scavenging activity compared with natural AOP (p < 0.05). This preparation method was at the international advanced level. Furthermore, pilot-scale production results showed that DSAOP was expressed successfully in fermenter cultures, which indicated that the design strategy and expression methods would be useful for obtaining substantial amounts of stable peptides at low costs. These results showed that DSAOP produced with recombinant Escherichia coli could be useful in cosmetic skin care products, health foods, and pharmaceuticals.

N,N-디아실, O-아실 키토산 올리고머의 물리화학적 특성에 관한 연구 (Study of Physico-Chemical Properties of N,N-Diacyl, O-Acyl Chitosan Oligomer)

  • 이옥섭;하병조;김준오;박성규;이윤식
    • 공업화학
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    • 제8권3호
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    • pp.365-373
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    • 1997
  • 천연 고분자인 키토산의 1차 아민기를 아질산 나트륨에 의해 oxidative-deamination 반응을 일으켜 reducing end에 알데히드기를 갖는 키토산 올리고머를 얻었으며, 이를 수소화붕소산나트륨으로 환원하여 2,5-anhydro-D-mannitol로 전환하였다. 얻어진 피토산 올리고머의 평균 중합도는 겔 투과 크로마토그래피를 통해 2정도임을 알 수 있었고, 키토산에 비해 친유성 유기 용매에 대한 높은 용해성을 나타내었다. 키토산 올리고머에 아실클로라이드를 4-디메틸아미노피리딘 촉매하에서 반응시켜 N,N-디아실, O-아실 키토산 올리고머를 합성하였다. 시차주사열량 분석을 실시한 결과 N,N-디라우로일, O-라우로일 키토산 올리고머의 경우 액정 영역을 나타내었고, 편광현미경을 통해 이를 확인할 수 있었다. X-선 회절분석 실험결과 키토산 올리고머와 달리 N,N-디라우로일, O-라우로일 키토산 올리고머는 높은 결정성 구조를 나타냄을 알 수 있었다.

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급속 진행성 사구체신염으로 시작된 현미경적 다발성 동맥염(Microscopic Polyangiitis) 환자의 7년간의 장기 추적관찰 1례 (Seven-Year Follow Up of Microscopic Polyangiitis Presenting with Rapidly Progressive Glomerulonephritis)

  • 오진원;김병길;이재승;정현주
    • Childhood Kidney Diseases
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    • 제12권1호
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    • pp.99-104
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    • 2008
  • 현미경적 다발성 동맥염은 폐출혈과 급속 진행성 사구체심염을 특징으로 하는 전신성 혈관염의 일종으로 소아에서는 매우 드문 질환이다. 저자들은 폐출혈과 급성 신부전을 동반한 7세 여아에서 신장조직검사와 p-ANCA 검사로 현미경적 다발성 동백염으로 진단 후 5년 뒤 2차 신장조직검사와 경구 ACE inhibitor, angiotensin II receptor blocker와 저용량의 cyclophosphamide를 투여 받은 환아를 7년간 추적관찰하였다. 발병당시에는 BUN 117 mg/dL, Cr 2.3 mg/dL이었으나, 퇴원시 BUN 20.8 mg/dL, Cr 1.6 mg/dL이었고, 최근 검사에서는 BUN 51.7 mg/dL, Cr 3.2 mg/dL으로 만성 신병증 소견을 보이고 있으며, 외래 추적 관찰 지속 중에 있다. 이에 문헌 고찰과 함께 증례 보고하는 바이다.

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Texture Analyzer (TA)를 이용한 화장품 크림의 In Vivo 끈적임 평가법의 최적화 (Optimization of In Vivo Stickiness Evaluation for Cosmetic Creams Using Texture Analyzer)

  • 류주연;배정은;강내규
    • 대한화장품학회지
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    • 제46권4호
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    • pp.371-382
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    • 2020
  • 화장품의 사용감을 관계 있는 물성의 측정을 통해 정량화하려는 시도가 이어져오고 있다. 그 중 끈적임은 texture ananlyzer를 이용하여 수직 힘을 측정하는 방식이 대표적이며, 시간에 따른 수직 힘의 그래프에서 음의 면적인 area under curve (AUC)와 상관관계를 갖는 것으로 알려져 있다. 최근 노르망디 대학에서는 이러한 특성에 피부의 특성을 함께 고려하여 TA를 이용한 in vivo 끈적임 평가법을 개발하였다[8]. 본 연구에서는 이를 확장하여 화장품 크림의 in vivo 끈적임 평가법을 최적화하고자 하였다. 페이셜 크림 5 종을 대상으로 크림의 도포량 및 도포 횟수, 탐침의 모양과 소재를 바꾸어 보면서 실험을 진행하였고, 관능 평가 결과를 기준으로 가장 부합하는 조건을 최적의 평가법으로 설정하였다. 그 결과, 3.4 cm의 원 내부에 70 μL의 크림을 7 s 동안 10 회 문지르고 측정하는 방식이 가장 적합한 것으로 판단되었다. 탐침의 경우, 원기둥형보다 구형의 탐침이 재현성이 높게 나타나 구형의 금속 탐침을 택하였다. 최적의 평가법을 확보하여 10 인의 피험자를 대상으로 인체 평가를 진행한 결과, 사람에 따른 절대값에는 차이가 있으나 AUC의 순위는 모두 같게 얻어졌다. 마지막으로 AUC의 끈적임 표준화의 시도로 PVP를 표준 물질로 설정하여 농도 별로 AUC를 측정하고, 5종의 크림 별 끈적임 인지율을 확인하여 AUC와 끈적임의 상관관계에 대해 알아보았다.