In this paper, it is aimed to determine the finite element model updating effects on the structural behavior of long span concrete highway bridges. Birecik Highway Bridge located on the 81stkm of Sanliurfa-Gaziantep state highway over Firat River in Turkey is selected as a case study. The bridge consist of fourteen spans, each of span has a nearly 26m. The total bridge length is 380m and width of bridge is 10m. Firstly, the analytical dynamic characteristics such as natural frequencies and mode shapes are attained from finite element analyses using SAP2000 program. After, experimental dynamic characteristics are specified from field investigations using Operational Modal Analysis method. Enhanced Frequency Domain Decomposition method in the frequency domain is used to extract the dynamic characteristics such as natural frequencies, mode shapes and damping ratios. Analytically and experimentally identified dynamic characteristics are compared with each other and finite element model of the bridge is updated to reduce the differences by changing of some uncertain parameters such as section properties, damages, boundary conditions and material properties. At the end of the study, structural performance of the highway bridge is determined under dead load, live load, and dynamic loads before and after model updating to specify the updating effect. Displacements, internal forces and stresses are used as comparison parameters. From the study, it is seen that the ambient vibration measurements are enough to identify the most significant modes of long span highway bridges. Maximum differences between the natural frequencies are reduced averagely from %46.7 to %2.39 by model updating. A good harmony is found between mode shapes after finite element model updating. It is demonstrated that finite element model updating has an important effect on the structural performance of the arch type long span highway bridge. Maximum displacements, shear forces, bending moments and compressive stresses are reduced %28.6, %21.0, %19.22, and %33.3-20.0, respectively.