Surface enhanced Raman scattering (SERS) is considered as one of promising medical and diagnostic technologies. The SERS effect is caused by the localized surface plasmon resonance (LSPR) from metal nanoparticles with narrow hot spots. The mechanism of LSPR, development of nanostructure fabrication, and corresponding researches are discussed. The flexible, label-free, low-cost, and highly-sensitive Au/ZnONRs/G is introduced. The Au/ZnONRs/G detects and distinguishes cataract, age-related macular degeneration, and diabetic macular edema from aqueous humor. Comprehension of SERS provides further improvement in bio sensing technology including early diagnosis and prolonged life expectancy.realize highly stretchable electrodes.