An experiment was performed to show the bio-signal characteristics of the Stuckyi when was heated. To measure the bio-signal of the plant, this study used the electrode with copper(+) and constantan(-). The electrode was directly inserted into the Stuckyi stems. And used T-type thermocouple to measure the inside temperature of the Stuckyi. During the experiments, room temperature also measured with T-type thermocouple. Heating was made with hair dryer periodically that 3 times turned on for heating 5 min and off for non-heating 25 min. Under the situation, the responses of the Stuckyi including voltage potential and inside temperature were measured using data logger(HP-34970A) according to the distances(15cm, 30cm, 45cm) between hair dryer and the plant. Through the experiments, We knew that the inside temperature has similar level and behavior with the room temperature at normal state. And during heating period, the inside temperature and the potential were also simultaneously increased sharply. From the experiments, we revealed that the measuring system of bio-signal of the plant being proposed in this study can be useful to show the Characteristics of the plants.