An Epidemiologic study was carried out on 77 TMD patients with degenerative joint disease who had visited the Orofacial Pain Clinic in Pusan National University Hospital. Al subjects were interviewed and examined clinically and radiologically using a standardized examination form. As related to gender and duration, subjective and objective sysmptoms in DJD patients were studied. The obtained results were as follows : 1. There were much more patients in the twenties or thirties, women and histories such as chronic duration and microtrauma. 2. Most patients responded positively more often to the questions of jaw function, unilateral chewing in habits, poot appetite and depression in behavioral response and shoulder pain in worsening prognosis 3. While the most common reasons for treatment were pain, noise, and limitation of opening, the associated symptoms such as headache, neckache, earache, jaw dysfunction, neck dysfunction, acute bite change and dizziness, ringing or fullness in the ears as secondary CNS excitatory effects were complained. 4. Opening the mouth in 25 to 40mm, soft end feel and deflective incisal pathway were seen and more tenderness to lateral or dorsal capsule of joint than intra or extra oral muscles were complained. 5. While there appeared no click, crepitus and single click in acute group, in chronic group, crepitus, single click and no click appeared in order of sequence. 6. Tomogram or bone scan revealed more bony changes than panorama and transcranial view.

To study the characteristics of EMG power spectrum of masticatory muscles during sustained isometric contraction and recovery at various contraction times, the author analysed the EMG signals of anterior temporal and masseter muscles before, during, and after sustained isometric contraction at 50% level of maximum voluntary contraction (MVC) for 15,30,60 seconds. Twelve normal subjects were included in this study. The author came to following conclusions from the results. 1. MMF of anterior temporal muscle in the contraction period was significantly higher than that of masseter muscle during sustained isometric contraction regardless of isometric contraction times (p<0.05). 2. MMF in the contraction period decreased as the contraction time increased during sustained isometric contraction in both temporal and masseter muscles(p<0.05). 3. SMF in the contraction period increased as the contraction tie increased during sustained isometric contraction in both temporal and masseter muscles(p<0.01). 4. MMF in the first part of recovery period (20 seconds) decreased as the contraction time increased during sustained isometric contraction(p<0.05). However, MMF in the later parts of recovery period (20-120 seconds) showed no significant differences. 5. MMF of anterior temporl muscle in the recovery period was significantly higher than that of masseter muscle after sustained isometric contraction regardless of isometric contraction times (p<0.05). 6. The recovery rate of MF reached 100% in 100 seconds after the isometric contraction regardless of isometric contraction times.

레이저가 의학영역에서 사용된 후 구강내 연조직과 경조직 병소에 대한 임상적 적용에 관한 관심이 커져왔다. 레이저가 조사되는 부위에서는 대부분 연기가 발생한다. 이 경우 병원체가 존재하는 병소에 대한 레이저치료시 발생되는 연기속에 병원체가 포함되어 이Tekas 환자와 술자는 이의 흡입가능성이 크므로 균혈증 및 상기도나 호흡계의 의원성 감염이 유발될 수 있다. 저자는 레이저치료시 발생되는 연기속에 병원체가 포함되어 있는지의 여부를 규명하기위해 각각 10개의 실험군에 통기성 미생물인 Escherichia coli(E.coli)가 배양된 brain geart infusion (BHI) 배지와 E.coli 가 존재하는 치근단 병소를 실험적으로 만든 후 각각 pulsed Nd:YAG 레이저를 조사해 연기를 채취, 배양, 분석하여 다음과 같은 결과를 얻었다. 1. Pulsed Nd:YAG 레이저를 E. colir 배양된 agar substrate 와 실험적으로 만들어진 치근단부위의 E.coli 현탁액에 조사시 발생된 연기에서 처음 접종된 것과 같은 E.coli가 검출되었다. 2. 감염병소에 pulsed Nd:YAG 레이저를 조사시 발생되는 연기의 유해효과로부터 환자와 술자 및 보조자의 보호를 위해 효과적인 흡입기를 사용이 권장된다.

Mandibular movement is composed of border movement and functional movement. Border movement such as maximal mouth opening, hinge opening ad lateral eccentric movement has good reproducibility, but functional movement such as chewing, swallowing and speech has also reproducibility. Especially for chewing movement, individual reproducibility has been confirmed by many studies. Study of chewing pattern is still in controversy. In new approach for raising the diagnostic value, numeric parameters and morphologic characteristics could be used for evaluation of chewing pattern. This study was performed to investigate the differences between chewing pattern in controls and in patients with temporomandibular disorders. Sixty-three patients with temporomandibular disorders participated in this study, and they were divided into unilaterally affected subjects or bilaterally affected subjects. Then unilaterally affected subjects were classified into closed lock group, disk displacement with reduction group, and degenerative joint disease group. For recording of chewing pattern, subjects were asked to chew one piece of presoftened chewing gum on both sides, and the chewing movement was recorded with the Electrognatho- Graphy(Bio-Research Associates Inc., U.S.A.). Tooth contact pattern for occlusal stability (Total left-right statistics )was also recorded with T-Scan(Tekscan Co., U.S.A.). The dta related to chewing pattern and total left-right statistics were statistically analyzed by SAS/stat program. The obtained results were as follows : 1. In patient group, mean value of A-P distance and the ratio of A-P distance to vertical distance were larger than control group, but the value of lateral distance in affected side and the closing velocity in unaffected side were smaller than that of control group, respectively. 2. In case of unilateral affected patients, chewing pattern of other side had tendency to restricted movement and slow velocity in closed lock group or degenerative joint disease group than control group or disk displacement with reduction group. 3. In bilateral degenerative joint disease patients, contralateral side had tendency to large range of motion and slow chewing velocity than preferred chewing side. 4. The patients with restricted mouth opening below than 35mm had higher value of total left-right statistics than patient group mouth opening above 35mm. Also closed lock group had higher total left-right statistics than disk displacement with reduction group, degenerative joint disease group and control group. 5. There was some difference in morphologic characteristics of chewing pattern between in control group and in affected side of unilateral patient group, but no difference between control group and unaffected side of unilateral patient group. 6. There were positive correlations between vertical distance and A-P distance, between vertical distance and chewing velocity, between A-P distance and chewing velocity, and between opening velocity and closing velocity in unilateral affected patients.

The purpose of this study was to assess the efficacy of several thermal therapies using ice pack, moist-hot pack and ultrasound, separately and concomitantly and to obtain the background information on the vascular changes after thermophysical therapies. The author had used 15 healthy subjects were examined and the subjects were divide into 5 groups : ice pack, moist-hot pack, ultrasound, ice pack and moist-hot pack, ice pack and ultrasound. Observation were made immediate before and 0,5,10,20,30,45,60,90 minutes after treatment. Thermography was performed in an Agema 870 thermovisio with 0.1$^{\circ}C$ difference of gradual temperature shift. The results were as follows : 1. Using ice pack only, the surface temperature of the masseter region was increased lapse of time, and most remarkably 90 minutes after the treatment. 2. Using moist-hot pack only, the surface temperature of the region was remarkably increased immediately after the treatment, but decreased lapse of time. 3. Using moist-hot pack with ice pack, the surface temperature of the face was remarkably increased immediately after the treatment, and decreased lapse of time, Hyperthermia was maintained for a longer time as compared with the group of moist-hot pack only. 4. Using ultrasound only, the surface temperature of the region was increased gradually, and most remarkably 30 minutes after the treatment, but decreased in the course of time. 5. Using ultrasound combined with ice pack, the surface temperature of the region was gradually decreased until 30 minutes after the treatment, and decrease to some extend at 45 minute. And then a gradual increase observed over the remaining period of the experiment. 6. Hyperthermia were maintained for a long time in the groups using ice pack combined with moist-hot pack and ultrasound as compared with the other groups. Our data suggest that ice pack can promote the efficacy of other thermal therapies.

This study was performed to investigate the mean life expectancy of dental prosthetic restorations. The author has examined 352 dental prosthesis clinically and radiologically, and decided the success(survival) and failure(mortality) of the dental prosthesis. The dental prosthesis which had been treated in the Seoul National University Dental Hospital, two private clinics in Seoul, one university dental hospital, and two private clinics in local province were included in this study. The survival analysis using product limit estimator was used and the mean life expectancy of each type of dental prosthesis was calculated. The results were as follows : 1. The life expectancies were 10.5 years in gold crown and bridge, 8.5 years in porcelain fused to metal crown and bridge, 8.3 years in nonprecious metal crown and bridge, 8.1 years in removal partial denture, and 7.7 years in full denture. 2. The causes of mortality were in the order of dental caries(24.6%), fracture of dental prosthesis(19.2%), periodontal problems(18.6%), chronic chewing difficulty and dysfunction due to dental prosthesis(15.0%), excessive exposure of abutments due to the marginal defect of dental prosthesis(14.4%), abnormal occlusion due to severe attrition of artificial teeth in dentures(3.0%), periapical problems(2.4%), perforation of dental prosthesis(1.8%), and loose contacts with neighboring tooth(1.2%). 3. Among survival cases, 66.5% showed normal chewing ability and 31.9% showed partial chewing ability. However, 1.6% of them complained loss of chewing ability. 4. Among failure cases, 6.6% showed normal chewing ability and 38.9% showed partial chewing ability. However, 54.5% of them complained loss of chewing ability.

In order to observe the influence of pulsed Nd:YAG laser at its out-of-contact with dentin on tooth temperature, we have applied pulsed Nd:YAG laser to 2mm thick dentin sample at a point of contact and from a distance of 1mm, 2mm, 3mm and 4mm with an energy of 0.3W, 0.5W, 0.8W, 1.5W and 2.0W. They were exposed to periods of 3 seconds, 6 seconds, 9 seconds and 15 seconds respectively and measured temperature changes. The results as follows : 1. When the time ad intensity of power were constant, the temperature changes on dentin of tooth depended on the distance. The temperature increased when the laser intensity increased bu two other conditions were contact. 2. At the point of contact, the temperature has risen over $5^{\circ}C$ regrdless of intensity of the power or the time. However, there was $5^{\circ}C$ fluctuation with 0.3 W for 3 seconds treatment. 3. The temperature change was less than $5^{\circ}C$ thermal change at the distance of 1mm and 2mm respectively when lased for 3 seconds, 6 seconds, 12 seconds and 15 seconds with 0.3 W. Similar results were observed at 3 and 6 seconds treatment with 0.5 W and at 3 seconds treatment with 0.8 and 1.0W respectively. 4. It showed less than 5(C thermal change when lased for 3 seconds, 6 seconds, 9 seconds, 12 seconds and 15 seconds with 0.3W at the distance of 3mm and 4mm. The same results were seen in 3 seconds, 6 seconds and 9 seconds treatment with 0.5W and in 3 seconds with 0.8W and 1.0W respectively. As we have seen the above, the results has indicated that pulsed Nd:YAG lasing at its off contact on dentin of 2mm thickness will not cause irreversible changes if lasing intensity, lasing distance and lasing time are appropriate.

Emotional state of dental outpatients was studied by means of symptom checklist-90- revision, 229 male and 290 female dental outpatients were subjected at the private local clinic in Pusna, during the period from 1994 to 1995. T-scores from SCL-90-R were analyzed psychologically by means of 90 questionnaires through Korean manual of SCL-90-R. The obntained results were as follows : 1. Mean value of T-scores on 9 basic scales of SCL-90-R in all the dental outpatients was within normal range. 2. T-scores in male were significantly higher than that in female, and there was a significant difference by age group in female. 3. There were higher levels of T-scores in groups of high academic carrier, salaryman, public servant, and unmarried person. 4. T-score of patients with periodontal pain was the highest in all subject groups. 5. While T-score of patients with K01(embedded and impated teeth) was the highest, that of K02(dental caries) was the lowest. 6. T-score in acute group was higher than that in chronic group. 7. T-score of patients treated in field of oral surgery or oral medicine was higher than those of other fields of dental treatments.

악관절장애환자의 임상 평가를 위해서 고안된 여러 지수 중 현재 가장 널리 사용되고 있는 두개하악장애지수와 평가방법을 새로 고안한 변형 두 개하악장애 지수, 혼합두개하악장애지수를 비교하여 환자의 증상변화를 민감하게 감지하는 능력을 알아보고자 본연굴를 시행하였다. 두개하악장애지수의 기준에 따라 단국대학교 치과병원 구강내과에 내원한 악관절장애환자 20명을 대상으로 임상검사를 시행하여 두개하악장애지수, 변형두개하악장애지수, 혼합 두 개하악장애 지수를 산출하였다. 치료전, 치료후 2주, 치료후 4주에 수집된 결과를 각 지수간에 비교분석하여 다음과 같은 결과를 얻었다. 악관절장애환자의 증상평가에서 변형두개하악장애지수가 환자의 증상변화를 가장 민감하게 감지하는 것으로 나타났다(P(0.05). 따라서 사용된 세가지 지수중 변형 두개하악장애지수가 악관절장애환자의 경과를 관찰할 때 증상변화를 관찰하기 위한 가장 효과적인 방법이라고 사료된다.

The purpose of this study was to investigate the relationship between the head and neck postre and the cervical curvature, especially in forward head postrue(FHP). Sixty patients with craniomandibular disorders and thirty dental students without any signs and symptoms of craniomandibular disordres participated in this study as patient groups and sa control group, respectively. The author evaluated the head and neck posture of all subjects by plumb line and CROM( Cervical Ragne of Motion), and had taken cephaograph in natural head position. On the cephalograph the angle of cervical inclination formed by true horizntal plane and 4th cervical vertebra(C4) and the radius of cervical curvature from C1 to C5 were measured. A specially designed ruler was used for measuring cervical curvature. Occlusal contac number and force with T-scan system, electromygraphic activity of cervical muscles with Bio-EMG, and distance of freeway space with Bio-ECN were recorded, respectively. The collected data were processed by SAS/STAT progrm. The obtained results were as follows : 1. In subjects with longer radius which was less cervical curvature, head positioned more anteriorly than subjects with smaller radius, and they showed slightly straight cervical vertebra. 2. Between the patients and the control group, there were no differences in cervical curvature, in forward head position by plumb line and in CROM. But the patient group had a greater cervical inclination than the control group had. 3. There were positive correlation between cervical curvature and forward head position by plumbline,between forward head position y plumb line and that by CROM in patient group. The cervical inclination, however, had negative correlation with cervical curvature, and with forward head postion by plumb line, respectively. 4. In case of showing more cervical curvature and more forward head position by plumb line the head position was defined as forward head posture. In patient group, subjects without forward head posture showed greater posterior teeth contact force than subjects with forward head posture, but in control group, there were no difference between the two subjects. 5. There were higher electromyographic activity in almost all muscles and smaller freeway space in induced forward head posture than those in natural head position in subjects without forward head posture. In conclusion, head position of patients with craniomandibular disorders were not more anterior than that of normal control person, but they had tendency to head extension. From the result of this study, forward head posture could be defined as posterior rotation of upper cervical segment with a straight lower cercial segment due to loss of normal lordosis.

이 연구의 목적은 측두하악관절통에 있어서 인도메타신 포노포레시스의 동통완화효과를 평가하기 위한 것이다. 측두하악관절통을 가진 20명의 환자들이 이 연구에 참여했다. 10명은 인도메타신을 이용하여 초음파치료를 받았고 다른 10명은 순수 초음파 치료용 젤을 이용하여 초음파 치료를 받았다. 동통완화효과를 평가하기 위하여 치료전과 치료후에 유추지수와 압력통각역치를 이중맹검법으로 평가하였다. 실험결과는 다음과 같다. 인도메다신을 이용하여 초음파 치료를 받은 군은 유추지수와 압력통각역치에서 다 유의한 동통의 감소를 보였다. 반면에 대조군에서는 치료후에 유추지수와 압력통각역치에서 동통의 감소를 보였지만 통계적으로 유의하지 않았다. 이 결과는 측두하악관절통에 있어서 단순한 초음파 치료보다 포노포레시스가 더 효과적이라는 것을 의미한다.

The purpose of this study is to document the stress symptoms (Anxiety and depression) among dental students and the difference of stress level between classes and by gender. All students in dental college, Seoul National University, in the 1995 academic year, were tested with the Spielberger's State-Trait Anxiety Inventory and Beck Depression Inventory just before the first semestral examination when the stress levels of students are expected to be the highest. 303 students answered the test. The obtained results were as follows : 1. State Anxiety inventory scores of the second-year and first-year students were significantly higher than those of the fourth -year and third-year(p(0.01), and all classes showed mean state anxiety scores more than mild. 2. Trait Anxiety inventory scores of the second-year and first-year students were significantly higher than those of the third-year and forth-year(p(0.01), and all classes showed mean Trait Anxiety scores more than mild. 3. Beck Depression inventory scores of the second -year students were significantly higher than those of the third-year and fourth-year(p(0.01), and twenty two students(7.3%) among the fourth classes showed depression symptoms. 4. In the comparison of the mean scores of the State-Trait Anxiety Inventory and Beck Depression Inventory between male and female students, State Anxiety Inventory scores of the second-year female students were significantly higher than those of the second year male students(p(0.05), and there was no significant difference elsewhere.

The author studied to determine the tooth-age according to the degree of attrition. This study was performed on 247 subjects over forties. Upper and lower posterior teeth were clinically examined and transferred to a store model on which degree of attrition was measured with a magnifier respectively. The obtained results were as follows : The attrition by aging was increased in both male and female. The degree of attrition in male was higher than that in female for same age group. The coefficients of correlation and relation equations by the degree of attrition were obtained.

This study was performed to evaluate and compare conservative treatment results by several parameters such as age, sex, symptom duration, type and timing of joint sound, parafunctional habits, splint type, and diagnostic classification. There have been too many articles reporting long term results of conservative treatment but articles related to comparison of treatment results by patients' self-evaluation have been rarely reported. For this study 258 patients with temporomandibular disorders(TMDs) were selected and examined by routine diagnostic procedure for TMDs. The subjects were classified into 5 TMDs subgroups ad treated with conservative treatments involving splints, physical modalities, jaw exercises, and counseling. Visual analogue scale(VAS) about pain, joint sound, and mouth opening limitation was recorded respectively during treatment period. From the VAS data and treatment duration, VAS treatment index(VAS Ti) was calculated. The obtained results were as follows : 1. Pain was the most frequent main symptom in subjects with temporomandibular disorders, and main symptom for mouth opening limitation was comparatively less than for pain or sound in disk displacement with reduction group or in degenerative joint disease group. 2. Degenerative joint disease group had the most poor treatment results and highest occlusal index of Helkim's index. 3. Good prognosis for conservative treatment was observed in acute group, under 6 months than chronic group, 6months over in symptom duration, and subjects with 40 years over in age showed the most poor prognosis. 4. Subjects treated with anterior repositioning splint had better treatment results than subjects treated with centric relation splint, but statistical significance in VAS Ti and treatment duration was not observed. 5. Treatment results according to affected side, types and point of joint sound did not show consistent statistical results. 6. The result for conservative treatment was observed poor in subjects with bruxism and clenching. 7. In studying coincidence between preferred chewing and affected side, frequency of preferred chewing side, in unilateral affection, was higher in ipsilateral than in contralateral side.

두개하악장애는 저작근, 측두하악관절 및 그와 관련된 구조물의 많은 임상적인 문제를 포함하는 포괄적인 용어이다. 두개하악장애의 원인은 다양하고 종종 다인성이며 위험을 증가시키는 여러가지 소인들이 존재한다. 이갈이나 이악물기 같은 이상기능습관은 흔하며 또한 임상적으로 많은 관련이 있는 것으로 사료된다. 그러나 이전의 연구에서는 연령증가에 따른 기능적 마모를 고려한 치아교모에 관한 연구가 시행되지 않고 단순한 교모도의 평가비교만이 시행되었다. 본 연구는 법치의학적 연령감정에서 널리 사용되고 있느 치아교모도를 이용한 연령감정법을 이용하여 두개하악장애환자의 치아교모도에 관한 연구를 시행하였다. 연령은 기능적마모에 따른 정도를 평가하기 위해서 고려되었다. 단국대학교 치과병원 구강내과에서 두개하악장애로 진단된 환자18명과 대조군으로 외래환자 14명을 대상으로 하였다. 연령차는 연령감정법의 추정연령과 환자의 실제나이의 차이를 구해서 비교분석하였다. 이상의 결과로 두개하악장애환자의 중절치, 측절치, 제1소구치, 제2소구치의 치아교모도가 정상군보다 유의성 있게 높았다.

This study was designed to determine the most effective concentration of fluoride and levels of laser irradiation for the remineralization of decayed teeth. After irradiation with a pulsed Nd:YAG laser and the topical application of fluoride, phosphate and fluoride concentration in enamel were measured. And then the changes on surface enamel using an scanning electron microscope were observed. Samples by extraction healthy, permanent, mandibular bovine teeth with no caries were obtained. Among them 371 healthy samples were selected and artificial carious lesions were made. 20 samples were assigned to each experimental group. After irradiation with a pulsed Nd:YAG laser with total energy densities of 10J/$\textrm{cm}^2$, 20J/$\textrm{cm}^2$ for each group. On the teeth, 2% NaF, 1.9% NH4F, 1.6% TiF4 Elmex gel(amine fluoride) and 1.23% APF gel were applied. After pH circulatory procedures, concentrations of fluoride with and Ionalyzer (Orion Research, Model 901, USA) and phosphates with an Uv/V is spectrophotometer (Uvikon 860, Kontrom Co, Switzerland) were measured. By etching the teeth in layers and measuring fluoride concentrations, a profile of fluoride penetration according to depth could be developed. And also the changes on the surface of the enamel using an electron scanning microscope were observed. The comparative analysis yielded the following results : 1. Phosphate concentration was low in all groups compared with the control group except for teeth treated Elmex gel, irradiated with 10J/$\textrm{cm}^2$ and 30J/$\textrm{cm}^2$ energy densities. Teeth treated with APF gel and 30J/$\textrm{cm}^2$ irradiation gad the lowest phosphate concentration. 2. Among all groups, fluoride concentrations in tooth enamel were highest in APF gel and NaF groups irradiated at 30J/$\textrm{cm}^2$. The APF gel group had the highest fluoride concentrations across all energy densities. 3. In the APF gel group, and the NaF group, the greater the energy density of the laser, the higher the fluoride concentrations in the enamel. 4. In all groups, the concentration of fluoride in the enamel by depth tended to decrease with depth. 5. Under the scanning electron microscope, under the condition of irradiation with 20J/$\textrm{cm}^2$, enamel crack was detected for the first time. In the NH4F group, spherical deposits were found on the surface of the enamel, and in the TiF4 group the surface of enamel was covered with an irregular, thin membranous mass in places. In the APF gel and NaF groups irradiated with 10J/$\textrm{cm}^2$, spherical and irregular particles covered the teeth. When these groups were irradiated at 20J/cm2, they were covered with amorphous crystals. These results suggest that one could obtain more effective anticariogenic effects without damage to teeth when less than 20J/$\textrm{cm}^2$ energy densities and APF gel are used.

To investigate the relationship between recurrent aphthous ulcer and oral mucosal keratinization, exfoliative cytology in buccal mucosa, lip mucosa, tongue mucosa were performed on 25 recurrent aphthous ulcer patients and 25 controls whose age ranged from 10 to 65. Keratinization cell ratio was then measured. The results were as follows : 1. Yellow cell ratio in the control group was more than that in the patient group in buccal mucosa, lip mucosa, tongue mucosa. Red cell ratio in the control group was more than that in the patient group in lip mucosa. Blue cell ratio in the patient group was more than that in control group in all regions( p(0.01) 2. In the comparison by sex, the patient group showed no significant difference in all site but, the control group showed different results according to the site; males were more than females in yellow cell, but less than females in red cell Females were more than males in yellow cell, but less than males in red cell. 3. In the comparison by age, patient group showed no significant difference in all site, but the control group showed significantly high yellow cell ratio in buccal and tongue mucosa over the age of 50. In conclusion, there was close relationship between recurrent aphthous ulcer and decreased oral mucosal keratinization. In other words, reduced oral mucosal keratinization must be recommended for prevention of recurrent aphthous ulcer.

The purpose of this study was to investigate the effect of alcohol and stress on liver and buccal mucosa in guinea pig by immunological methods. Especially, Cytochrome P450 (CYP) which in oxidase during alcohol metabolism and bioactivator to carcinogen was used as an indicator in this study. 48 guinea pigs were used in this study. The experimental guinea pig were divided into three groups: The first was a group with giving alcohol-15%(v/v) ethyl alcohol, the second group was a with giving stress in the $0^{\circ}C$ water and the third was a control group. Every 4 guinea pigs of each group were sacrificed weekly-first, second, third, fourth week after experiment and extracted liver tissues and buccal mucosa. The liver tissues were observed by using immunoblotting technique (Western blot) and buccal mucosa were observed by immunofluorescence technique. The results were as follows: 1. By the alcohol and stress, Cytochrome P450 was amplified positive in the liver tissues at third week. 2. By the alcohol and stress, Cytochrome P450 was not detected in the buccal mucosa at any period.

The purpose of this study was to assess the thermographic differences of craniomandibular area between normal individual and patients with craniomandibular disorders and to compare the differences between clinical and thermographical assessment. The author had used 50 subjects as materials for this study, which was divided into 2 groups (first group included 15 healthy subjects and second group included 35 patients) with craiomandibular disorders; 17 subjects had normal disc-condyle relationship, 13 subjects had disc displacement with reduction and 5 subjects had disc displacement without reduction. Agema 870 thermovision(D.I.T.I.) was used to take thermographs with $0.1^{\circ}C$ difference of gradual temperature shift. The results were as follows : 1. Of 34 patients with craniofacial pain, 15(44%) subjects showed hyperthermia on the pain site in the thermography, 8(23.5%)exhibited hyperthermia on the site opposed to the pain site, and 11(32.4%) did not show any significant thermal change. One patient without craniofacial pain showed hyperthermia on the site opposed to the site of disc displacement without reductin. 2. Of 35 patients with craniofacial pain or disc displacement, 24(68.6%) subjects showed a significant thermal difference between symptomatic and asymptomatic sides of the face, but 11(31.4%) did not show any difference. 3. Of 17 patients with pain but with normal disc-condyle relationship, 8(47.1 subjects showed hyperthermia on the pain site, 4(23.5%) showed on the site opposed to the pain site, and 5(29.4%) did not show any significant thermal change. 4. Or 13 patients with pain and disc displacement with reduction, 6(46.2%) subjects showed hyperthermia on the pain site, 3(23.1%) showed on the site opposed to the pain site, and 4(30.8%) did not show any significant thermal change. 5. 15 healthy subjects did not show any thermal differences between the both sides of the face.

The purpose of this study was to evaluate an effect of change on head posture initial occlusal contacts with measuring the distances between initial occlusal contacts and maximum intercuspal position at different head posture in TMDs patient. For this study, 24 patients from age 13 to 36 were selected, they were examined health history taken, patients who have sign and symptoms of TMDs were examine before the study. For the normal group, 21 adults from age 23 to 25 were selected. They have normal or class I molar relationship, and have no other prosthetic restorations. Difference on distance between initial occlusal contact and maximum intercuspal position with mandibular kinesiograph$(MKG^R)$(K6 diagnostic system, Myo-tronic Inc, USA) in upright, supine, 45$^{\circ}$ extension, 30$^{\circ}$ flexion position of the head were measured. The Frankfort horizontal plane was used as a reference plane. The results were as follows : 1. There were significant differences between initial occlusal contacts of the normal and patient group on upright position and 30$^{\circ}$ flexion of the head(p<0.05, p<0.01) 2. The position of the initial occlusal contacts have a tendency to place anterior and inferior to maximal intercuspal position in upright position and 30( flexion of the head as well as posterior and inferior in supine position and 45$^{\circ}$ extension of the head in the normal and patient groups. 3. There were significant differences among the initial occlusal contacts between uptight and supine position; upright and 45$^{\circ}$ extension of the head(p<0.05); supine position and 30$^{\circ}$ flexion of the head, .and 30(flexion and 45$^{\circ}$ extension of the head in the patient group(p<0.01) The result have shown that after treatment on the supine position, it may be necessary to check occlusal contact on the upright position as well ass flexion of the head. It may need careful adjustment in occlusal condition on upright position of TMDs patient.

Human genomic Deoxyribonucleic acid(DNA) was extracted from teeth by boiling, salting-out, phenol, boiling-phenol methods. The author compared DNA concentration and its purity, the accuracy of sex determination and the results of the D1S80 locus detection among above 4 methods. The following results were obtained : 1. DNA concentration was the highest in pulp with salting-out method and DNA purity was higher in pulp with salting-out and phenol methods than other 2 methods. 2. Sex determination was possible using of the pulp and the dentin of the teeth with four methods but, it was impossible in the enamel and some pulp with boiling method. 3. Amplification of D1S80 locus occurred from pulp and dentin with salting-out, phenol, and boiling-phenol methods. 4. There are no differences among the amplification of X-Y homologus amelogenin gene by application of 4 methods and salting-out, phenol methods efficiently makes available to amplification of D1S80 locus. From the investigation DNA extraction, sex determination, amplification of D1S80 locus was successfully accomplished with salting-out, phenol, boiling-phenol methods Therefore above 3 methods are available and applicable as forensic odontology for individual identification.

The human genomic deoxyribonucleic acid(DNA) was extracted from the pulp, dentin of 22 teeth by clelex, phenol methods. Samples of the tooth-derived DNA were amplified by polymerase chain reaction(PCR), electrophosed for sex determination by detection of X-Y homologus amelogenin gene and D1S80 locus detection The following results have been achieved. 1. Chelex and phenol method are effective to sex determination in the pulp and dentin 2. Chelex method is not suitable for detection of D1S80 locus. 3. Concentration and purity of DNA for teeth using chelex method is lower than using phenol method. From the above investigation, chelex method is simple, rapid for sex determination, but it is not suitable for detection of VNTRs.