According to the increase of population and development of industrialization air and water pollution problems are still keeping going to great nuisance to human activities. Specially man should drink 2l clean water to maintain our health every day, but we afraid of drink the city tap water because of the contaminants like heavy metals, bacteria trihalomethane, etc. In the analysis of the anions in potable water, we usually adapt the Standard methods for the Examination of Water and Wastewater. But this method is tedious and time consuming, so the Ion Chromatography method is now used in research of water quality. Author worked with Ion Chromatography in measuring the anions in drinking water by attaching conductivity dector to normal High Performance Liquid Chromatograph. Low-capacity ion-exchange coulmn and dilute eluents, 0.00M phthalic aic was used in this study. The concentration of chloride ion was 1.55 ppm$\~$3 8.81ppm, nitrate ion was 5.45 ppm$\~$18.27ppm, and sulfate ion was 19.64 ppm$\~$28.86 ppm. The phosphate ion was detected only in Apt. tap water as 167.99 ppm whose amount was supposed to be used as a water pipe cleaner.

Having studied the production of monoclonal antibodies for developing a diagnosis medicine which shall be detected by a high-sensitivity test by using Rhodotorula rubra as a fungi-host which had been extracted through biochemical tests and follow-up examinations on Yeast-like fungi obtained from pulmonary tissues of pulmonary tuberculosis patients who had been in Kong ju National Tuberculosis Hospital from Jun. to Dec. in 1987, I. have gained such results as follows: 1. The fusion rate was influenced by feeder cell layers, cell density and time required to the cell fusion with cells in myelona subculture. 2. The fusion rate did not show any significant difference when the cell was applyed with two molecular weights, i.e., 1500 and 4000, of polyethylene glycol. 3. Fused cells after the addition of HAT selection media were bright and round, whereas unfused myelona cells and spleen cells were shrunk and granulated. 4. The cell fusion rate turned out to be about $57.2\%$(150 wells / 264 wells). 5. $10\%$(15 wells / 150 wells) of the positive reaction was detected in monoclonal antibody screening. 6. The titer which had reacted positively to Rhodotorula rubra fungal-host was 800 times in density after the gradual dilution of the produced monoclonal antibodies with Indirect ELISA method. 7. The Strongest specific reaction came out after the peroxidase labelled anti-human Immunogobulin had been applyed to Rhodotorula rubra for activating its nature after making drift with Carbonate-bicarbonate buffer (pH 9.6) and drying completely.

The purpose of this investigation was performed for chemical values related to rancidity of fat and oils extracted from deep-frying food in Seoul. The samples were collected according to kinds of fat and oils, sampling sites and food stuffs for frying. The results were as follows 1. The most used frying oils were rice bran oil $(37.0\%)$ and shortening $(29.6\%)$, followed by rapeseed oil $(14.8\%)$. 2. The chemical values related to rancidity of fat and oils extracted from deep-frying foods were the next on average. Acid value-1.42, peoxide value-39.82, dinitrophenyl hydrazine value-16.73 thiobarbituric acid value-0.263. However the highest value among oils was showed in rice bran oil and the lowest value was shortening. 3. For the classification of sampling sites, acid value and peroxide value of samples of the markets were found higher than them of environs of school and roadside, and the differences between them were significant (P> 0.01), while dinitrophenyl hydrazine value and thiobar bituric acid value were not showed the significant differences. 4. For the classification of foodstuffs for frying, there were not recognized significant differences among all kinds of them.

Management of Swimming pool is focused on Swimming pool samitation in relation with chlorination of swimming water and potable water, disposal of waste and excrement within the boundry of swimming pool that may be summerised as follows: 1. Chlorination of Swimming Water Residual chlorine must be kept within the range of $0.4\~0.6 mg/l$ and in case of chloramine should be $0.7\~1.0mg/l$ while swimming pool is in operation 2. Chlorination of potable Water Residual chlorine must be kept within the rangs of $0.2\~04 mg/l$ at all time 3. Disposal of litters must be kept in the water tight waste bin with fitted lid, and waste should not be overflow -out of the bin. When waste in landfilled, the sufficient amount of cover material should be used daily. 4. Disposal of excrement Toilet must be water-flush type. However, The establishment of pit latrine is unavoidable the excrement must be covered with lime or dirt so that excrement should not be exposed to air.

A synthetic wastewater composed of powdered baby food and inorganic nutrients was treated by five 3.6L anaerobic reactors in order to test the relationship between solids retention time (SRT) and media surface ratio, and the removal efficiencies of organic substrate. Of the five reactors, four were semi-continuously fed stirred-tank reactors and one continuously-stirred batch reactor. The influent was 7430mg/L in COD, 7120 mg/L $BOD_L$ and 6350mg/L in $BOD_5$, respectively. Operating temperature was $35{\pm}1^{\circ}C$ and pH in the range 6.9 to 7.2. In this experimental study it was found that a linear relationship existed, within the experiment range, between SRT and media surface ratio, and that SRT and removal efficiency increased with increasing media surface ratio. The substrate removal efficiencies were 82.7 to $88.2\%$ in COD, 82.9 to $88.4\%$ in $BOD_L$ and 83.3 to $88.7\%$ in $BOD_5$, respectively.

This study was performed to investigate the contents of heavy metals in Anguilla japonica habiting in Korea. The samples were collected along by Nak Tongriver, Young San river and culture farms. Lead, Cadmium, Copper, Zinc, Manganese, Chromium were tested by the Atomic Absoption Spectrop-hotometer after wet digestion, Mercury was tested by using mercury analyzer. The results were as follows: 1. Aresenic, Copper contents in the four parts were the higest values in liver 2. In total mercury contents of the each parts, the higest value was detected in muscle. 3. In all metals, Zinc and Manganese were detected with high contents, Mecury and Cadmium were detected with low contents. Lead, Cadmium, Zinc, chromiumi, Manganese were detected with high in bone. 4, The difference was not significant between natural and feeding Anguilla Japonica, but significant correlation with parts.