Purpose: The purpose of this study was to identify the impacts of professional socialization perceived by nursing students on role conflict and professional self-concept. Methods: Using a structured questionnaire, data were collected from 683 nursing students. For data analysis, descriptive statistics, Pearson correlation coefficient, stepwise multiple regression were conducted using SAS program. Results: The mean score of total professional socialization was 3.74 followed by the scores of 'independence', 3.98, 'commitment', 3.80, 'decision making', 3.60, and 'maturity', 3.53. The mean score of total role conflict was 3.72, and professional self-concept was 2.75. Total professional socialization, commitment, and independence perceived by nursing students were positively related to role conflict. Also total professional socialization and total subcategories of professional socialization were positively related to professional self-concept. As a result of stepwise multiple regression analysis, the key determinants of role conflict were found to be 'independence', 'motivation with admission', 'sex', 'desire with working', 'grade' and 'decision making'. The key determinants of professional self-concept were 'decision making', 'commitment', 'satisfaction about nursing', and 'interpersonal relationship'. Conclusion: Through this results, it is necessary to improve professional socialization in order to increase professional self-concept among nursing students.