Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Monitoring of infectious diseases in three grouper species (Hyporthodus septemfasciatus, Epinephelus moara and E. moara ♀× E. lanceolatus ♂) cultured in recirculating aquaculture systems

순환여과시스템에서 양식되는 바리과 어류 3종[능성어, (Hyporthodus septemfasciatus), 자바리(Epinephelus moara), 교잡종 대왕자바리(E. moara ♀× E. lanceolatus ♂)]의 감염성 질병 모니터링

  • Hee-Jae Choi (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Da-Yeon Choi (Department of Aquatic Life Medicine, Kunsan National University) ;
  • SungHyun Jo (Department of Aquatic Life Medicine, Kunsan National University) ;
  • JeYun Shin (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Jong Yeon Park (Daejeon Regional institute of industrial advancement) ;
  • In-chul Bang (Department of Life Science & Biotechnology, Soonchunhyang University) ;
  • Yue Jai Kang (Department of Aquatic Life Medicine, Kunsan National University)
  • 최희재 (군산대학교 수산생명의학과) ;
  • 최다연 (군산대학교 수산생명의학과) ;
  • 조성현 (군산대학교 수산생명의학과) ;
  • 신제윤 (군산대학교 수산생명의학과) ;
  • 박종연 (대전지역산업진흥원) ;
  • 방인철 (순천향대학교 생명과학과) ;
  • 강예재 (군산대학교 수산생명의학과)
  • Received : 2024.05.21
  • Accepted : 2024.06.12
  • Published : 2024.06.30
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Abstract

The Serranidae is high-quality fish species with good meat quality and is traded at high price, and is attracting attention in South Korea as a cultured species that creates high added value. However, the high-density fish farming for mass production increases the risk of mass mortality due to infectious diseases, leading to enormous economic losses. Therefore, in order to safely prevent and protect farmed fish from serious infectious diseases, it is necessary to conduct disease monitoring on a regular basis. In this study, Hyporthodus septemfasciatus, Epinephelus moara, and the hybrid longtooth grouper (E. moara ♀×E. lanceolatus ♂) were collected once a month from fish farm of Garorim and Aquabiotech Co., Ltd for a total of six months, from July to December 2023. We investigated infections of five species of bacterial diseases, including Flavobacterium columnare, six species of viral diseases, including LCDV (lymphocystis disease virus), and parasitic pathogens in grouper farms. As the result, Vibrio vulnificus and V. harveyi were detected in H. septemfasciatus in August, in the case of viral diseases, NNV was detected in H. septemfasciatus from July to August using RT-PCR or PCR. Finally, In the case of parasitic diseases, Tricodina sp. was detected in E. moara and the hybrid longtooth grouper from August to December.

Keywords

Acknowledgement

이 논문은 2023년도 정부(해양수산부)의 재원으로 해양수산과학기술진흥원-해양수산 신산업기술사업화(R&D)의 내수시장 활성화 사업 지원을 받아 수행된 연구임(RS-2023-000243369).

References

  1. Adel, M., Amiri, A. B., Dadar, M., Breyta, R., Kurath, G., Laktarashi, B. and Ghajari, A.: Phylogenetic relationships of Iranian infectious hematopoietic necrosis virus of rainbow trout (Oncorhynchus mykiss) based on the glycoprotein gene, Archives of virology. 161: 657-663, 2016. https://doi.org/10.1007/s00705-015-2684-8
  2. Bandin, Isabel and Sandra, S.: Betanodavirus and VER disease: a 30-year research review, Pathogens. 9: 106, 2020. https://doi.org/10.3390/pathogens9020106
  3. Boo, M. S., Lee, C. H., Kim, B. H., Kim, M. S., Kim, D. G., Baek, H. J., Kim, H. B. and Lee, Y. D.: Management and development of seed rearing in red spotted grouper Epinephelus akaara, Korean journal of fisheries and aquatic sciences. 53: 506-514, 2020. https://doi.org/10.5657/KFAS.2020.0506
  4. Bregnballe, J.: A guide to recirculation aquaculture, 2022. https://doi.org/10.4060/cc2390en
  5. Bunlipatanon, P. and U-taynapun, K.: Growth performance and disease resistance against Vibrio vulnificus infection of novel hybrid grouper (Epinephelus lanceolatus× Epinephelus fuscoguttatus), Aquaculture research. 48: 1711-1723, 2017. https://doi.org/10.1111/are.13008
  6. Cheng, A.-C., Cheng, S.-A., Chen, Y.-Y. and Chen, J.-C.: Effects of temperature change on the innate cellular and humoral immune responses of orange-spotted grouper Epinephelus coioides and its susceptibility to Vibrio alginolyticus, Fish & shellfish immunology. 26: 768-772, 2009. https://doi.org/10.1016/j.fsi.2009.03.011
  7. Cho, M.-Y., Park, G.-H., Ji, B.-Y. and Kim, J.-W.: Statistical data on fish virus of cultured olive flounder, Paralichthys olivaceus from 2005 to 2007, Journal of fish pathology. 23: 155-163, 2010.
  8. Chun, J., Huq, A. and Colwell, R. R.: Analysis of 16S-23S rRNA intergenic spacer regions of Vibrio cholerae and Vibrio mimicus, Applied and environmental microbiology. 65: 2202-2208, 1999. https://doi.org/10.1128/AEM.65.5.2202-2208.1999
  9. Colwell, R.: Polyphasic taxonomy of the genus Vibrio: numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species, Journal of bacteriology. 104: 410-433, 1970. https://doi.org/10.1128/jb.104.1.410-433.1970
  10. Dixon, P., Paley, R., Alegria-Moran, R. and Oidtmann, B.: Epidemiological characteristics of infectious hematopoietic necrosis virus (IHNV): a review, Veterinary research. 47: 1-26, 2016. https://doi.org/10.1186/s13567-016-0341-1
  11. FishStatJ, F.: A tool for fishery statistics analysis, FAO Fisheries and aquaculture department, FIPS-Statistics and information: Rome, Italy. 2017. Harikrishnan, R., Balasundaram, C. and Heo, M.-S.: Fish health aspects in grouper aquaculture, Aquaculture. 320: 1-21, 2011. https://doi.org/10.1016/j.aquaculture.2011.07.022
  12. Harikrishnan, R., Balasundaram, C. and Heo, M.-S.: Fish health aspects in grouper aquaculture, Aquaculture. 320: 1-21, 2011. https://doi.org/10.1016/j.aquaculture.2011.07.022
  13. Harikrishnan, R., Kim, J.-S., Balasundaram, C. and Heo, M.-S.: Immunomodulatory effects of chitin and chitosan enriched diets in Epinephelus bruneus against Vibrio alginolyticus infection, Aquaculture. 326: 46-52, 2012. https://doi.org/10.1016/j.aquaculture.2011.11.034
  14. Hassan, A., Khan, I., Abdulmawjood, A. and Lammler, C.: Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis, Journal of clinical microbiology. 39: 1618-1621, 2001. https://doi.org/10.1128/jcm.39.4.1618-1621.2001
  15. Hawley, L. M. and Garver, K. A.: Stability of viral hemorrhagic septicemia virus (VHSV) in freshwater and seawater at various temperatures, Diseases of aquatic organisms. 82: 171-178, 2008. https://doi.org/10.3354/dao01998
  16. Hoihuan, A., Soonson, P., Bunlipatanon, P., Thawonsuwan, J., Tanasomwang, V., Areechon, N. and Unajak, S.: Molecular genotyping and phenotyping of Vibrio vulnificus isolated from diseased, brown-mar- bled grouper (Epinephelus fuscoguttatus) in Thailand with preliminary vaccine efficacy analysis, Aquaculture. 545: 737188, 2021. https://doi.org/10.1016/j.aquaculture.2021.737188
  17. Hu, Y.-q., Wang, W.-y., Fath, T., Li, F.-x., Fang, L.-f., Zhou, Z.-h. and Zhang, D.-f.: Rapid and simultaneous detection of viable Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae by PMA-mPCR assay in aquatic products, LWT. 180: 114663, 2023. https://doi.org/10.1016/j.lwt.2023.114663
  18. Huang, H.-Y., Chen, Y.-C., Wang, P.-C., Tsai, M.-A., Yeh, S.-C., Liang, H.-J. and Chen, S.-C.: Efficacy of a formalin-inactivated vaccine against Streptococcus iniae infection in the farmed grouper Epinephelus coioides by intraperitoneal immunization, Vaccine. 32: 7014-7020, 2014. https://doi.org/10.1016/j.vaccine.2014.08.039
  19. Huang, X., Huang, Y., Xu, L., Wei, S., Ouyang, Z., Feng, J. and Qin, Q.: Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China, Journal of fish diseases. 38: 379-387, 2015. https://doi.org/10.1111/jfd.12244
  20. Hwang, J. Y., Lee, U. H., Heo, M. J., Kim, M. S., Jeong, J. M., Kim, S. Y., Kwon, M. G., Jee, B. Y., Kim, K. H., Park, C. I. Park, J. W.: Naturally occurring substitution in one amino acid in VHSV phosphoprotein enhances viral virulence in flounder. PLoS pathogens, 17(1), e1009213.(2021), 2021. https://doi. org/10.1371/journal.ppat.1009213
  21. Jang, M. H., Lee, N.-S., Cho, M. and Song, J.-Y.: Status and characteristics of JEECV (Japanese eel endothelial cell-infecting virus) and AnHV (Anguillid herpesvirus 1) infections in domestic farmed eels Anguilla japonica, Anguilla bicolor and Anguilla marmorata, Korean journal of fisheries and aquatic Sciences. 54: 668-675, 2021. https://doi.org/10.5657/KFAS.2021.0668
  22. Kang, M. J., Noh, C. H., Kim, J. H., Park, J. Y. and Park, D. W.: The Embryonic Development and Hatchability of Two Hybrids with Giant Grouper Female: Giant Grouper♀× Kelp Grouper♂ and Giant Grouper♀× Red-Spotted Grouper♂, Korean journal of fisheries and aquatic sciences. 53: 557-562, 2020. https://doi.org/10.5657/KFAS.2020.0557
  23. Kim, K.-R., Moon, S.-J., Park, J.-Y., Huynh, D. T., Park, J.-Y., Kim, K.-S., Han, S.-B. and Bang, I.-C.: Effects of salinity, water temperature and development stage on the hatching rate and survival of fertilized eggs in hybrid grouper (Epinephelus fuscoguttatus♀× E. lanceolatus♂) for long-distance transport, Ocean and polar research. 40: 161-167, 2018. https://doi.org/10.4217/OPR.2018.40.3.161
  24. Kim, Y. B., Okuda, J., Matsumoto, C., Takahashi, N., Hashimoto, S. and Nishibuchi, M.: Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene, Journal of clinical microbiology. 37: 1173-1177, 1999. https://doi.org/10.1128/jcm.37.4.1173-1177.1999
  25. Kim, Y. H., Park, J. Y., Kim, J. H. and Bang, I.-C.: Molecular Biological Species Identification of Imported Groupers (Epinephelus moara♀× E. lan-ceolatus♂), Korean journal of fisheries and aquatic sciences. 53: 566-571, 2020. https://doi.org/10.5657/KFAS.2020.0566
  26. Lindholm-Lehto, P.: Water quality monitoring in re- circulating aquaculture systems, Aquaculture, fish and fisheries. 3: 113-131, 2023. https://doi.org/10.1002/aff2.102
  27. Ma, H., Wen, W., Su, Y., Feng, J., Xu, L., Peng, C. and Guo, Z.: Epidemiological characterization of VNNV in hatchery-reared and wild marine fish on Hainan Island, China, and experimental infection of golden pompano (Trachinotus ovatus) juveniles, Archives of virology. 160: 2979-2989, 2015. https://doi.org/10.1007/s00705-015-2590-0
  28. Ma, H., Xie, J., Weng, S., Zhou, T. and He, J.: Co-infection of megalocytivirus and viral nervous necrosis virus in a very severe mass mortality of juvenile orange-spotted groupers (Epinephelus coioides), Aquaculture. 358: 170-175, 2012. https://doi.org/10.1016/j.aquaculture.2012.06.032
  29. Madsen, H. C., Buchmann, K. and Mellergaard, S.: Trichodina sp.(Ciliophora: Peritrichida) in eel Anguilla anguilla in recirculation systems in Denmark: host-parasite relations, Diseases of aquatic organisms. 42: 149-152, 2000. https://doi.org/10.3354/dao042149
  30. Martins, C., Eding, E. H., Verdegem, M. C., Heinsbroek, L. T., Schneider, O., Blancheton, J.-P., d'Orbcastel, E. R. and Verreth, J.: New developments in recirculating aquaculture systems in Europe: A perspective on environmental sustainability, Aquacultural engineering. 43: 83-93, 2010. https://doi.org/10.1016/j.aquaeng.2010.09.002
  31. Megarajan, S., Ranjan, R., Xavier, B., Ghosh, S., Sadu, N., Bathina, C. and Gopalakrishnan, A.: Molecular detection of betanodavirus in orange-spotted grouper (Epinephelus coioides) broodstock maintained in recirculating aquaculture systems and sea cages, Aquaculture international. 28: 349-362, 2020. https://doi.org/10.1007/s10499-019-00466-3
  32. Natnan, M. E., Low, C.-F., Chong, C.-M., Rungrassamee, W. and Baharum, S. N.: The Effect of Oleic Acid-Enriched Diet in Hybrid Groupers (Epinephelus fuscoguttatus× Epinephelus lanceolatus) upon Infection with Vibrio vulnificus Using an LC-qTOFMS Approach, Journal of marine science and engineering. 11: 1563, 2023. https://doi.org/10.3390/jmse11081563
  33. Nhung, P. H., Shah, M. M., Ohkusu, K., Noda, M., Hata, H., Sun, X. S., Iihara, H., Goto, K., Masaki, T. and Miyasaka, J.: The dnaJ gene as a novel phylogenetic marker for identification of Vibrio species, Systematic and applied microbiology. 30: 309-315, 2007. https://doi.org/10.1016/j.syapm.2006.11.004
  34. Nishizawa, T., Mori, K-I., Nakail T., Fusawa I., Muroga, K.: Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis virus (SJNNV). Diseases of aquatic organisms, 18, 103-107, 1994.
  35. Panicker, G., Myers, M. L. and Bej, A. K.: Rapid detection of Vibrio vulnificus in shellfish and Gulf of Mexico water by real-time PCR, Applied and environmental microbiology. 70: 498-507, 2004. https://doi.org/10.1128/AEM.70.1.498-507.2004
  36. Park, C.-K.: The Mass Production of Fertilized Eggs for Industrial Aquaculture of the Convict Grouper Hyporthodus septemfasicatus, Korean journal of fisheries and aquatic sciences. 54: 31-37, 2021. https://doi.org/10.5657/KFAS.2021.0031
  37. Park, J. Y., Kim, Y. H. and Bang, I.-C.: Comparison of Morphological Characteristics and Its Parent Species Hybrid Grouper between a Epinephelus moara♀ and E. lanceolatus♂, Korean journal of fisheries and aquatic sciences. 53: 572-576, 2020. https://doi.org/10.5657/KFAS.2020.0572
  38. Ranjan, R., Megarajan, S., Xavier, B., Dash, B., Ghosh, S., Menon, M. and Edward, L. L.: Conditioning, maturation and year-round natural spawning of orangespotted grouper, Epinephelus coioides (Hamilton, 1822), in recirculating aquaculture system, Aquaculture research. 48: 5864-5873, 2017. https://doi.org/10.1111/are.13409
  39. Rimmer, M. A. and Glamuzina, B.: A review of grouper (Family Serranidae: Subfamily Epinephelinae) aquaculture from a sustainability science perspective, Reviews in aquaculture. 11: 58-87, 2019. https://doi.org/10.1111/raq.12226
  40. Roach, J. C. M., Levett, P. N. and Lavoie, M. C.: Identification of Streptococcus iniae by commercial bacterial identification systems, Journal of micro- biological methods. 67: 20-26, 2006. https://doi.org/10.1016/j.mimet.2006.02.012
  41. Sah Putra, B., Hick, P. M., Hall, E., Whittington, R. J., Khairul, R., Evarianti, Nurbariah and Becker, J. A.: Prevalence of infectious spleen and kidney necrosis virus (ISKNV), nervous necrosis virus (NNV) and ectoparasites in juvenile Epinephelus spp. farmed in Aceh, Indonesia, pathogens. 9: 578, 2020. https://doi.org/10.3390/pathogens9070578
  42. Shen, G., Shi, C., Fan, C., Jia, D., Wang, S., Xie, G., Li, G., Mo, Z. and Huang, J.: Isolation, identificationand pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus× Epinephelus lanceolatus, Journal of fish diseases. 40: 1351-1362, 2017. https://doi.org/10.1111/jfd.12609
  43. Verma, D. K., Rathore, G., Pradhan, P. K., Sood, N. and Punia, P.: Isolation and characterization of Flavobacterium columnare from freshwater ornamental goldfish Carassius auratus, Journal of environmental biology. 36: 433, 2015.
  44. Wang, L., Tian, Y., Cheng, M., Li, Z., Li, S., Wu, Y., Zhang, J., Ma, W., Li, W. and Pang, Z.: Transcriptome comparative analysis of immune tissues from asymptomatic and diseased Epinephelus moara naturally infected with nervous necrosis virus, Fish & shellfish immunology. 93: 99-107, 2019. https://doi.org/10.1016/j.fsi.2019.07.020
  45. Won, K. M., Lee, J. T., Cho, M. Y., Kim, M. S., Kim, N. Y., Jung, S. H. and Lee, N. S.: A case study of mortality caused by viral encephalopathy and retinopathy (VER) in cultured sevenband grouper, Epinephelus septemfasciatus during Winter, Korean journal of ichthyology. 29: 157-164, 2017.
  46. Yang, S. G., Ji, S. C., Moon, T. S., Son, M. H., Kim, K. M., Hur, S. P., Lee, C. H. and Lee, Y. D.: Adaptive characteristics of the longtooth grouper Epinephelus bruneus according to stocking density and water temperature, Korean journal of fisheries and aquatic sciences. 47: 847-852, 2014. https://doi.org/10.5657/KFAS.2014.0847
  47. Zorriehzahra, M. J.: Viral nervous necrosis disease, Emerging and reemerging viral pathogens. 673-703, 2020. https://doi.org/10.1016/B978-0-12-819400-3.00030-2