한국균학회지 (The Korean Journal of Mycology)

  • 제50권3호
  • /
  • Pages.149-160
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  • 2022
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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해금강과 금강상류 남대천 및 금산천 주변으로부터 야생효모의 분리 및 국내 미기록 효모들의 균학적 특성

Isolation and Determination of Microbiological Characteristics of Unrecorded Wild Yeasts from Waters and Soils of Haegeumgang in the Southern Sea, and from Namdaecheon and Geumsancheon Upstream of Geumgang, Korea

  • 문정수 (배재대학교 의생명과학과) ;
  • 이향범 (전남대학교 농생명화학과) ;
  • 이종수 (배재대학교 의생명과학과)
  • Jeong-Su Moon (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Hyang-Burm Lee (Department of Agricultural Biological Chemistry, Chonnam National University) ;
  • Jong-Soo Lee (Faculty of Biomedicine and Biotechnology, Paichai University)
  • 투고 : 2022.08.25
  • 심사 : 2022.09.29
  • 발행 : 2022.09.30
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초록

남해안과 금강상류 주변환경의 야생효모들의 효모 종 분포특성을 조사하고 국내 미보고된 효모들을 선별하여 이들의 균학적 특성들을 알아보고자 남해안의 해금강과 금강상류인 전북 무주의 남대천 및 금산의 금산천 주변의 물과 토양들로부터 야생효모들을 분리, 동정하였다. 해금강 주변 시료 35점에서 22종 52균주의 야생효모들을 분리, 동정하였고 무주의 남대천 주변 물과 토양 30점에서는 24 종 43균주의 야생효모들을, 금산의 금산천변 시료 60점에서 45종 79균주의 야생효모들을 분리, 동정하였다. 이들 지역에서 분리, 동정한 야생효모들중 Exobasidium rhododendri HHG10-5 (NNIBR2022633FG1), Udeniomyces pyricola NDC29-1 (NNIBR2022633FG2), Pichia scaptomyzae BAC2-3 (NNIBR2022633FG4), Diddensiella caesifluorescens GSC2-2 (NNIBR2022633FG5) 등 4균주들이 국내 미기록 야생효모들로 최종 선별되었다. 이들의 균학적 특성을 조사한 결과Udeniomyces pyricola NDC29-1 과Diddensiella caesifluorescens GSC2-2 균은 구형이었고 Exobasidium rhododendri HGG10-5 와 Pichia scaptomyzae BAC2-2 균들은 난형이었으며 Pichia scaptomyzae BAC2-3 균주만이 포자를 생성하였다. Udeniomyces pyricola NDC29-1 균주 외에는모두 vitamin-free배지에서도생육이아주양호하였고Pichia scaptomyzae BAC2-3, Diddensiella caesifluorescens GSC2-2 균주는 40% glucose 를함유한 YPD배지에서도생육하는내당성균이었다. Udeniomyces pyricola NDC29-1 균주를 제외한 나머지 균들은 Ca 등의 중금속등에 대하여 강한 내성을보였다.

The goal of this study was to investigate the diversity of wild yeasts from the waters and soils of Haegeumgang in Gyungsangnam-do, and Namdaecheon and Geumsancheon in upstream of Geumgang, Korea and to characterize any previously unrecorded wild yeast strains. In total, 52 strains comprising 22 different species of wild yeasts were isolated from 35 samples obtained from Haegeumgang. Forty three and sevent nine wild yeast strains were isolated from 90 samples taken from Namdaecheon and Geumsancheon, respectively. Among the total 174 isolated wild yeast strains, 4 strains, i.e., Exobasidium rhododendri HGG10-5 (NNIBR2022633FG1), Udeniomyces pyricola NDC29-1 (NNIBR2022633FG2), Diddensiella caesifluorescens GSC2-2 (NNIBR2022633FG5) and Pichia scaptomyzae BAC2-3 (NNIBR2022633FG4) were previously unrecorded yeasts were oval or spherical in shape, only Pichia scaptomyzae BAC 2-3 formed ascospores. Three strains with the exception of Udeniomyces pyricola NDC 29-1 grew well in vitamin-free medium and Exobasidium rhododendri HGG 10-5 grew well in YPD medium containing 10% NaCl. All four novel strains assimilated fructose, lactose, raffinose, starch and xylose.

키워드

과제정보

This work was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea.

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