The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

First Report of Macrophomina phaseolina Causing Charcoal Rot in Bottle Gourd in Korea

  • Kim, Sang Gyu (Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Tae Bok (Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Lee, Oak Jin (Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • Received : 2021.07.02
  • Accepted : 2021.09.17
  • Published : 2021.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Stem blight symptoms were observed in greenhouse-grown bottle gourds (Lagenaria siceraria) in Wanju, Korea. Infected plants showed leaf chlorosis and wilting; the plants eventually died with the infected leaves remaining attached, and black sclerotia formed on the diseased stem. Based on the morphological characteristics and pathogenicity results and sequence analyses of the internal transcribed spacer, translation elongation factor 1-α, β-tubulin, and calmodulin regions, the isolated fungus was Macrophomina phaseolina. This is the first report of charcoal rot in Lagenaria siceraria caused by Macrophomina phaseolina in Korea.

Keywords

Acknowledgement

This study was supported by a grant (Project No. PJ01260801) funded by the National Institute of Horticultural and Herbal Science, Rural Development Administration.

References

  1. Davis AR, Perkins-Veazie P, Hassell R, Levi A, King SR, Zhang X. Grafting effects on vegetable quality. HortScience 2008;43:1670-2. https://doi.org/10.21273/hortsci.43.6.1670
  2. King SR, Davis AR, Liu W, Levi A. Grafting for disease resistance. HortScience 2008;43:1673-6. https://doi.org/10.21273/hortsci.43.6.1673
  3. Lee JM, Kubota C, Tsao SJ, Bie Z, Echevarria PH, Morra L, Oda M. Current status of vegetable grafting: Diffusion, grafting techniques, automation. Sci Hortic 2010;127:93-105. https://doi.org/10.1016/j.scienta.2010.08.003
  4. Turhan A, Ozmen N, Kuscu H, Serbeci MS, Seniz V. Influence of rootstocks on yield and fruit characteristics and quality of watermelon. Hortic Environ Biotechnol 2012;53:336-41. https://doi.org/10.1007/s13580-012-0034-2
  5. Karaca F, Yetisir H, Solmaz I, Candir E, Kurt S, Sari N, Guler Z. Rootstock potential of Turkish Lagenaria siceraria germplasm for watermelon: Plant growth, yield and quality. Turk J Agric For 2012;36:167-77.
  6. Farr DF, Rossman AY. Fungal databases [Internet]. Washington, D.C.: U.S. National Fungus Collections, ARS, USDA; 2021. [cited 2021 Jan 7]. Available from https://nt.ars-grin.gov/fungaldatabases/
  7. Mertely J, Seijo T, Peres N. First report of Macrophomina phaseolina causing a crown rot of strawberry in Florida. Plant Dis 2005;89:434. https://doi.org/10.1094/PD-89-0434A
  8. Qamar MI, Ghanzafar MU, Abbas MF, Zainab R, Andleeb S, Shafiq M, Mushtaq S. First report of Macrophomina phaseolina causing crown and root rot on strawberry in Sargodha, Pakistan. Plant Dis 2019;103:1420.
  9. White TJ, Bruns TD, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications. In: Innis MA, Gelfand DH, editors. London: Academic Press; 1990. p. 315-22.
  10. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999;91:553-6. https://doi.org/10.2307/3761358
  11. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microb 1995;61:1323-30. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  12. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547-9. https://doi.org/10.1093/molbev/msy096
  13. Ko YM, Choi J, Lee YH, Kim HT. First report of charcoal rot caused by Macrophomina phaseolina on Glycine max in Korea. Res Plant Dis 2020;26:29-37. https://doi.org/10.5423/RPD.2020.26.1.29