The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Development of New Strains of Wolfiporia cocos for Sclerotium Formation by 2-Way Cross-Breeding

이원교배에 의해 균핵 형성하는 복령 균주 개발

  • Ka, Kang-Hyeon (Department of Forest Bioresources, National Institute of Forest Science) ;
  • Kim, Suyeon (Department of Forest Bioresources, National Institute of Forest Science) ;
  • Park, Mi-Jeong (Department of Forest Bioresources, National Institute of Forest Science) ;
  • Jeong, Yeun Sug (Department of Forest Bioresources, National Institute of Forest Science) ;
  • Ryoo, Rhim (Department of Forest Bioresources, National Institute of Forest Science) ;
  • Jang, Yeongseon (Department of Forest Bioresources, National Institute of Forest Science) ;
  • Choi, Jong-Woon (Gangwon Forest Research Institute) ;
  • Kim, Seong Hwan (College of Science & Technology, Dankook University)
  • 가강현 (국립산림과학원 산림생명자원연구부) ;
  • 김수연 (국립산림과학원 산림생명자원연구부) ;
  • 박미정 (국립산림과학원 산림생명자원연구부) ;
  • 정연석 (국립산림과학원 산림생명자원연구부) ;
  • 유림 (국립산림과학원 산림생명자원연구부) ;
  • 장영선 (국립산림과학원 산림생명자원연구부) ;
  • 최종운 (강원도산림과학연구원) ;
  • 김성환 (단국대학교 과학기술대학)
  • Received : 2021.07.21
  • Accepted : 2021.09.23
  • Published : 2021.09.30
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Abstract

Wolfiporia cocos is an important medicinal fungus that has been used in regions of Northeast Asia including Korea, Japan, and China. W. cocos is classified in Korea into two types (red bokryeong and white bokryeong) based on the internal colors (yellow orange-pale pink and white) of the sclerotium. Generally, the W. cocos type cultivated on farms produces white sclerotium. In this study, we endeavored to select strains that form sclerotium in sawdust medium using 2-way cross-breeding among two cultivated strains and three wild strains. Monospores were isolated from the fruiting bodies of cultivated and wild strains on potato dextrose agar. Thirty-nine strains of 338 hybrid strains isolated formed sclerotia with white or yellow colors upon culture for 3 months in Pinus densiflora sawdust medium. Selection for sclerotium forming strains using sawdust culture follows a very simple and easy procedure that is presented for the first time in this paper. We plan to test selected strains in the field to aid in developing new varieties for the future.

복령은 한국, 일본, 중국을 포함한 동북아시아 지역에서 사용되어 왔던 중요한 약용버섯이다. 복령은내부색깔을기준으로적복령과백복령으로구분하며, 일반적으로 백복령이 농가에서 재배되고 있다. 본 연구에서는 2가지 재배 복령과 3가지 야생 복령을 이용하여 이원교배를 통해 만들어진 균주로부터 톱밥배지에서 균핵을 형성하는 균주를 선발하려고 하였다. 단포자는 PDA 배지에서 만들어진 복령의 자실체로부터 분리하였다. 분리된 교잡균주 338개 중 39개가 소나무 톱밥배지에서 3개월 동안 배양하는 과정에서 백색 또는 황색의 균핵을 형성하였다. 톱밥배지를 이용한 균핵 형성 균주의 선발은 매우 간단하고 쉬운 방법이며 본 논문에서 처음으로 제시하는 것이다. 그리고 선발된 균주는 향후 새로운 품종을 개발하고자 야외실험을 시도할 계획이다.

Keywords

Acknowledgement

This study was supported by the grants (FP0800-2020-02) from the National Institute of Forest Science, Republic of Korea.

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