Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The in vitro and in vivo Safety Evaluation of Lactobacillus acidophilus IDCC 3302

  • Bang, Won Yeong (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Chae, Seung A (Ildong Bioscience) ;
  • Ban, O-Hyun (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Oh, Sangki (Ildong Bioscience) ;
  • Park, Chanmi (Ildong Bioscience) ;
  • Lee, Minjee (Ildong Bioscience) ;
  • Shin, Minhye (Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Science, Seoul National University) ;
  • Yang, Jungwoo (Ildong Bioscience) ;
  • Jung, Young Hoon (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2020.11.18
  • Accepted : 2020.12.15
  • Published : 2021.03.28
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Abstract

As consumption of healthy foods continues to garner remarkable public attention, ensuring probiotic safety has become a priority. In this study, the safety of Lactobacillus acidophilus IDCC 3302 was assessed in vitro and in vivo. L. acidophilus IDCC 3302 showed negative results for hemolytic and β-glucuronidase activities. The whole-genome analysis (WGA) revealed that L. acidophilus IDCC 3302 did not possess antibiotic resistance or virulence genes. The minimal inhibitory concentrations of L. acidophilus IDCC 3302 confirmed its safety concerning antibiotic resistance. Furthermore, L. acidophilus IDCC 3302 was demonstrated to be nontoxic in the oral toxicity test in rats. Therefore, the results suggested that L. acidophilus IDCC 3302 might be safe for human consumption.

Keywords

Acknowledgement

This work was supported by Ildong Bioscience, Co., Ltd., and the National Research Foundation of Korea (NRF) grant funded by Korea government (Ministry of Science and ICT, MSIT) [grant number 2020R1C1C1005251].

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