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http://dx.doi.org/10.48022/mbl.2011.11004

The in vitro and in vivo Safety Evaluation of Lactobacillus acidophilus IDCC 3302  

Bang, Won Yeong (School of Food Science and Biotechnology, Kyungpook National University)
Chae, Seung A (Ildong Bioscience)
Ban, O-Hyun (School of Food Science and Biotechnology, Kyungpook National University)
Oh, Sangki (Ildong Bioscience)
Park, Chanmi (Ildong Bioscience)
Lee, Minjee (Ildong Bioscience)
Shin, Minhye (Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Science, Seoul National University)
Yang, Jungwoo (Ildong Bioscience)
Jung, Young Hoon (School of Food Science and Biotechnology, Kyungpook National University)
Publication Information
Microbiology and Biotechnology Letters / v.49, no.1, 2021 , pp. 39-44 More about this Journal
Abstract
As consumption of healthy foods continues to garner remarkable public attention, ensuring probiotic safety has become a priority. In this study, the safety of Lactobacillus acidophilus IDCC 3302 was assessed in vitro and in vivo. L. acidophilus IDCC 3302 showed negative results for hemolytic and β-glucuronidase activities. The whole-genome analysis (WGA) revealed that L. acidophilus IDCC 3302 did not possess antibiotic resistance or virulence genes. The minimal inhibitory concentrations of L. acidophilus IDCC 3302 confirmed its safety concerning antibiotic resistance. Furthermore, L. acidophilus IDCC 3302 was demonstrated to be nontoxic in the oral toxicity test in rats. Therefore, the results suggested that L. acidophilus IDCC 3302 might be safe for human consumption.
Keywords
Antibiotic resistance; Lactobacillus acidophilus; probiotics; safety evaluation;
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