한국물환경학회지 (Journal of Korean Society on Water Environment)

  • 제37권6호
  • /
  • Pages.449-457
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  • 2021
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  • 2289-0971(pISSN)
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  • 2289-098X(eISSN)
한국물환경학회 (Korean Society on Water Environment)
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미세조류 활성도 평가를 위한 Photosynthesis and Respiration (P&R) Analyzer 분석조건 최적화

Photosynthesis and Respiration (P&R) Analyzer Analysis Optimization for Microalgal Activity Evaluation

  • 허재희 (경희대학교 일반대학원 환경응용과학과) ;
  • 심태석 (경희대학교 일반대학원 환경응용과학과) ;
  • 황선진 (경희대학교 일반대학원 환경응용과학과)
  • Huh, Jae-Hee (Department of Applied Environmental Science, Kyung Hee University) ;
  • Sim, Tae-Suk (Department of Applied Environmental Science, Kyung Hee University) ;
  • Hwang, Sun-Jin (Department of Applied Environmental Science, Kyung Hee University)
  • 투고 : 2021.10.05
  • 심사 : 2021.11.18
  • 발행 : 2021.11.30
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초록

Photosynthesis and respiration rate of microalgae are important factors during advanced wastewater treatment research using microalgae, There are several equipments and measurement methods for measuring photosynthesis and respiration, with different challenges that occur during pretreatment and stabilization of the analysis process. Therefore, in this study, for accurate Photosynthesis and Respiration (P&R) analyzer measurement, the analysis process was divided into pre-processing, DO stabilization, and analysis stages and each was optimized to enable accurate evaluation. For this purpose, the effect of DO saturation of the sample on P&R analysis, DO stabilization according to the degassing flow rate, and photoinhibition of the OD level on photosynthesis was investigated. Based on our study results, when DO was supersaturated, photosynthetic efficiency decreased due to photorespiration, making it inappropriate as a P&R sample. In addition, 0.5 L-N2/min level was the optimal nitrogen degassing flow rate for DO desaturation. The inhibition of photosynthetic efficiency by self-shading caused by the increase in OD was observed from OD 2.0, and it was found that P& R analysis is preferably performed on samples with OD less than 2.0. In addition, based on the above three optimization results, an optimized P&R Analyzer instruction for accurate P&R analysis was also presented.

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과제정보

본 연구는 이 논문은 2018년도 정부(미래창조과학부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구이며(No. NRF-2019R1A2C1084155) 이에 감사드립니다.

참고문헌

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