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http://dx.doi.org/10.15681/KSWE.2021.37.6.449

Photosynthesis and Respiration (P&R) Analyzer Analysis Optimization for Microalgal Activity Evaluation  

Huh, Jae-Hee (Department of Applied Environmental Science, Kyung Hee University)
Sim, Tae-Suk (Department of Applied Environmental Science, Kyung Hee University)
Hwang, Sun-Jin (Department of Applied Environmental Science, Kyung Hee University)
Publication Information
Journal of Korean Society on Water Environment / v.37, no.6, 2021 , pp. 449-457 More about this Journal
Abstract
Photosynthesis and respiration rate of microalgae are important factors during advanced wastewater treatment research using microalgae, There are several equipments and measurement methods for measuring photosynthesis and respiration, with different challenges that occur during pretreatment and stabilization of the analysis process. Therefore, in this study, for accurate Photosynthesis and Respiration (P&R) analyzer measurement, the analysis process was divided into pre-processing, DO stabilization, and analysis stages and each was optimized to enable accurate evaluation. For this purpose, the effect of DO saturation of the sample on P&R analysis, DO stabilization according to the degassing flow rate, and photoinhibition of the OD level on photosynthesis was investigated. Based on our study results, when DO was supersaturated, photosynthetic efficiency decreased due to photorespiration, making it inappropriate as a P&R sample. In addition, 0.5 L-N2/min level was the optimal nitrogen degassing flow rate for DO desaturation. The inhibition of photosynthetic efficiency by self-shading caused by the increase in OD was observed from OD 2.0, and it was found that P& R analysis is preferably performed on samples with OD less than 2.0. In addition, based on the above three optimization results, an optimized P&R Analyzer instruction for accurate P&R analysis was also presented.
Keywords
Microalgae; Photosynthesis rate; Photorespiration; P&R analyzer; Respiration rate;
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