대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)

  • 제35권1호
  • /
  • Pages.40-47
  • /
  • 2021
  • /
  • 1226-8038(pISSN)
  • /
  • 2287-9463(eISSN)
대한한방소아과학회 (The Association of Korean Oriental Pediatrics)
권호 표지
DOI QR코드

DOI QR Code

황금추출물의 지방장벽 생성을 통한 염증완화 효과

Effect of Anti-Inflammation through Creation of Skin Fat Barrier on Scutellaria baicalensis extract

  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 김기봉 (부산대학교 한의학전문대학원 한방소아과학교실)
  • Ahn, Sang Hyun (Dept. of anatomy, college of Korean Medicine, Semyung University) ;
  • Kim, Ki Bong (School of Korean Medicine, Pusan National University)
  • 투고 : 2020.12.21
  • 심사 : 2021.02.22
  • 발행 : 2021.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives The purpose of this study is to confirm the effect of alleviating inflammation through creating skin fat barrier from Scutellaria baicalensis extract. Methods Four-week-old Balb/C mice were divided into four groups: control group (Ctrl), lipid barrier eliminated group (LBEG), dexamethasone (DM) treated group after lipid barrier elimination (DMTG), and Scutellaria baicalensis (SB) treated group after lipid barrier elimination (SBTG). Scutellaria baicalensis extract were administered for 5 days after removal of the fat barrier. Changes in skin condition, improvement of the fat barrier, and relief of inflammation were observed in each group. Results Compared to LBEG and DMTG, pathological skin damage and tissue changes were less in SBTG, and transepidermal water loss (TEWL) and pH were also significantly reduced. Filaggrin was also significantly increased in SBTG. KLK7, PAR-2, and TSLP in SBTG also showed significant reduction compared to the LBEG and DMTG. Conclusions Scutellaria baicalensis extract restores skin barrier and relieves inflammation through the creation of skin fat barrier. This means that the Scutellaria baicalensis extract can regulate Th2 differentiation through the creation of the epithelial fat barrier.

키워드

참고문헌

  1. Elias PM, Wakefield JS. Skin barrier function. Curr Allergy Asthma Rep. 2008;8(4):299-305. https://doi.org/10.1007/s11882-008-0048-0
  2. Matsui T, Amagai M. Dissecting the formation, structure and barrier function of the stratum corneum. Int Immuno. 2015;27(6):269-280. https://doi.org/10.1093/intimm/dxv013
  3. Egawa G, Kabashima K. Barrier dysfunction in the skin allergy. Allegology International. 2018;67(1):3-11. https://doi.org/10.1016/j.alit.2017.10.002
  4. Yosipovitch G, Misery L, Proksch E, Metz M, Stander S, Schmelz M. Skin Barrier Damage and Itch: Review of Mechanisms, Topical Management and Future Directions. Acta Dermato-Venereologica. 2019;99(13):1201-9.
  5. Takei K, Mitoma C, Hashimoto-Hachiya A, Takahara M, Tsuji G, Nakahara T, Furue M. Galactomyces fermentation filtrate prevents T helper2-mediated reduction of filaggrin in an aryl hydrocarbon receptor-dependent manner. Clin Exp Dermatol. 2015;40(7):786-93. https://doi.org/10.1111/ced.12635
  6. Takei K, Mitoma C, Hashimoto-Hachiya A, Uchi H, Takahara M, Tsuji G, Kido Nakahara M, Nakahara T, Furue M. Antioxidant soybean tar Glyteer rescues T-helper-mediated downregulation of filaggrin expression via aryl hydrocarbon receptor. J Dermatol. 2015;42(2):171-180. https://doi.org/10.1111/1346-8138.12717
  7. Tenn MW, Ellis AK. The clinical relevance of filaggrin mutations: Effect on allergic disease. Ann Allergy Asthma Immunol. 2016 Nov;117(5):483-489. https://doi.org/10.1016/j.anai.2016.03.027
  8. The textbook compilation committee of Korean herbology. Bonchohak. Seoul. Younglimsa. 2010:218-20.
  9. Park WS. Effect of Scutellariae Radix Water Extract on Hydrogen Peroxide Production in RAW 264.7 mouse macrophages. Kor. J. Herbology. 2011;26(1):53-8. https://doi.org/10.6116/KJH.2011.26.1.053
  10. Yang BD, Kim SR, Choi CH, Jeoung HW, Kim HW. Effects of Scutellaria baicalensis Extract on Skin Lesion of Contact Dermatitis induced by DNFB in Mice. J Physiol & Pathol Korean Med. 2017;31(1):59-64. https://doi.org/10.15188/kjopp.2017.02.31.1.59
  11. Kim YH, Park YS. Effect of Scutellaria baicalensis Water Extract on Antioxidative Activity and Epidermal Thickness in DNCB-induced Allergic Contact Dermatitis Animal Model. Korean Soc Food Sci Nutr. 2006;35(5):543-8. https://doi.org/10.3746/jkfn.2006.35.5.543
  12. Kim BA, Kim MS, Kang BM, Byeon SH, Park IH, Park JH, Jung JW, Ahn EM, Jung HA, Jang JH, Bae W, Lee HY, Choi PN, Park CI. Inhibitory Studies of Hwangryunhaedok-tang on Development of Atopic Dermatitis in NC/Nga Mice. Kor. J. Herbology. 2008;23(2):59-65.
  13. Jun JH, Kang YH. The Effects of Scutellaria baicalensis GEORGI on Allergic contact dermatitis induced by DNCB in Mice. D.J.I.O.M. 1998;7(1):119-33.
  14. Smeden J, Bouwstra JA. Stratum corneum lipids: their role for the skin barrier function in healthy subjects and atopic dermatitis patients. Curr Probl Dermatol. 2016;49:8-26. https://doi.org/10.1159/000441540
  15. Kubo A, Nagao K, Amagai M. Epidermal barrier dysfunction and cutaneous sensitization in atopic diseases. J Clin Invest 2012;122:440-7. https://doi.org/10.1172/JCI57416
  16. Sandilands A, Sutherland C, Irvine AD, McLean WHI. Filaggrin in the frontline: role in skin barrier function and disease. J Cell Sci. 2009 May 1; 122(9):1285-1294. https://doi.org/10.1242/jcs.033969
  17. Brown SJ, McLean WH. One remarkable molecule: Filaggrin. J Invest Dermatol. 2012;132(3):751-62. https://doi.org/10.1038/jid.2011.393
  18. Choi EH, Yoon NY. Pathogenesis of atopic dermatitis. J Korean Med Assoc. 2014;57(3):218-225. https://doi.org/10.5124/jkma.2014.57.3.218
  19. Lee SE, Jeong SK, Lee SH. Protease and protease-activated receptor-2 signaling in the pathogenesis of atopic dermatitis. Yonsei Med J. 2010;51(6):808-22. https://doi.org/10.3349/ymj.2010.51.6.808
  20. Yamamoto M, Miyai M, Matsumoto Y, Tsuboi R, Hibino T. Kallikrein-related peptidase-7 regulates caspase-14 maturation during keratinocyte terminal differentiation by generating an intermediate form. J Biol Chem. 2012;287(39):32825-34. https://doi.org/10.1074/jbc.M112.357467
  21. Akdis M, Akdis CA. IgE class switching and cellular memory. Nat Immunol. 2012;13(4):312-4. https://doi.org/10.1038/ni.2266
  22. Amin K. The role of mast cells in allergic inflammation. Respir Med. 2012;106(1):9-14. https://doi.org/10.1016/j.rmed.2011.09.007
  23. Metcalfe DD, Baram D, Mekori YA. Mast cells. Physiol Rev. 1997;77(4):1033-79. https://doi.org/10.1152/physrev.1997.77.4.1033
  24. Cha HY, Ahn SH, Cheon JH, Park IS, Kim JT, Kim K. Hataedock Treatment Has Preventive Therapeutic Effects in Atopic Dermatitis-Induced NC/Nga Mice under High-Fat Diet Conditions. Evid Based Complement Alternat Med. 2016;1739760.
  25. Klemm S, Ruland J. Inflammatory signal transduction from the Fc epsilon RI to NF-κB. Immunobiology. 2006;211(10):815-20. https://doi.org/10.1016/j.imbio.2006.07.001