DOI QR코드

DOI QR Code

찔레꽃(Rosa multiflora) 추출물과 Ascorbic Acid 의 시너지 효과를 통한 항산화 활성과 총 페놀함량 및 플라보노이드 함량 분석

Analysis of Antioxidant Activity and Total Phenol Content and Flavonoid Content Through the Synergistic Effect of Rosa multiflora Extracts and Ascorbic Acid

  • 범석현 (대구한의대학교 화장품약리학과) ;
  • 권현지 (대구한의대학교 화장품약리학과) ;
  • 현진아 (대구한의대학교 화장품약리학과) ;
  • 강은빈 (대구한의대학교 화장품약리학과) ;
  • 박하은 (대구한의대학교 화장품약리학과) ;
  • 한동근 (대구한의대학교 화장품약리학과) ;
  • 김현정 ((주)허니스트) ;
  • 최은영 (대구한의대학교 화장품약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Beom, Seok-Hyun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Kwon, Hyun-Ji (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Hyun, Jin-A (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Kang, Eun-Bin (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Park, Ha-Eun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Han, Dong-Geun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Kim, Hyun-Jeong (R&D Center, HONEST.Co.,Ltd.) ;
  • Choi, Eun-Young (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • An, Bong-Jeon (Department of Cosmeceutical Science, Daegu Hanny University)
  • 투고 : 2021.12.01
  • 심사 : 2021.12.30
  • 발행 : 2021.12.30

초록

본 연구에서 찔레꽃을 추출, 농축 및 동결 등을 통하여 시료화 하였다. 찔레꽃추출물과 ascorbic acid의 복합물과 단일 ascorbic acid의 항산화 효능을 비교하였다. 본 연구의 목적은 찔레꽃추출물과 ascorbic acid의 시너지 효과를 통한 항산화 효능을 분석하는 것이었다. 실험은 전자공여능, ABTS+ radical 소거능, SOD 유사활성, xanthine oxidase 저해 활성, 환원력, FRAP, 폴리페놀 함량 및 플라보노이드 함량을 측정하였다. 그 결과, 전자공여능 실험과 xanthine oxidase 저해 활성 실험에서 복합물이 대조군(ascorbic acid)보다 높은 항산화 활성을 보였다. 플라보노이드 함량 분석 실험의 경우, 100 ㎍/mL의 농도에서 RMW+A (272.1 mg QE/g), RME+A (90.6 mg QE/g), RMW+A (79.4 mg QE/g)이 대조군(19.0 mg QE/g)보다 함량이 더 높았다. 따라서 찔레꽃추출물과 ascorbic acid 복합물은 단독 사용한 경우에 비하여 시너지 효과를 발휘했다. 따라서 이 복합물은 항산화 화장품에 사용할 경우 제품의 안정화 및 피부노화 억제에 기여할 것으로 사료된다.

In this study, Rosa multiflora was sampled through extraction, concentration, and freezing. The antioxidant efficacy of a mixture of R. multiflora extract and ascorbic acid was compared with a single ascorbic acid. The purpose of this study was to analyze the antioxidant efficacy through the synergistic effect of R. multiflora extract and ascorbic acid. The experiment measured electron donating ability, ABTS+ radical scavenging, SOD-like activity, xanthine oxidase inhibition activity, reducing power, FRAP, polyphenolic content measurement, and flavnoid content measurement. As a result, the complex showed higher antioxidant activity than the control group (ascorbic acid) in the electron donating ability test and the xanthine oxidase inhibitory activity test. In the case of the flavonoid content experiment, it was confirmed that RMW+A (272.1 mg QE/g), RME+A (90.6 mg QE/g), and RMW+A (79.4 mg QE/g) at a concentration of 100 ㎍/mL had a higher flavonoid content than the control group (19.0 mg QE/g). Therefore, the extract and ascorbic acid complex exhibited a synergistic effect compared to the single use. Therefore, it is thought that this complex will contribute to product stabilization and skin aging inhibition when used in antioxidant cosmetics.

키워드

과제정보

본 연구는 "대구한의대학교 2021학년도 교내 연구비 지원"에 의해 수행되었으며 이에 감사드립니다.

참고문헌

  1. M. Aritomi, On the components of the flower petals of Rosa multiflora Thunb. and Rubus hirsutus Thunb, J. P. Soc. Japan, 82, 771 (1962).
  2. J. S. Yeo, S. S. Chun, and J. H. Choi, Antioxidant activites of solvent extracts from Rosa multiflora, J. Life. Sic, 24(11), 1217 (2014). https://doi.org/10.5352/JLS.2014.24.11.1217
  3. R. Tsao, Chemistry and biochemistry of dietary polyphenols, Nutrients 2(12), 1231 (2010). https://doi.org/10.3390/nu2121231
  4. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radical. Biol. Med, 26(9-10), 1231 (1999). https://doi.org/10.1016/S0891-5849(98)00315-3
  5. M. S. Blois, Antioxidant determination by the use of stable free radical, Nature, 181, 1199 (1958). https://doi.org/10.1038/1811199a0
  6. S. Marklund and G. Marklund, Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase, Eur. J. Biochem, 47(3), 468 (1974).
  7. M. Oyaizu, Studies on product of browning reaction : Antioxidative activities of products of browning reaction prepared from glucosamine, Japanese. J. Nutrition, 44(6), 307 (1986). https://doi.org/10.5264/eiyogakuzashi.44.307
  8. J. Carmichael, W. G. Degraff, A. F. Gazdar, J. Minna, and J. B. Michell, Evaluation of a tetrazolium-based semiautomated colorimetric assay, assessment of chemosensitivity testing, Cancer. Res, 47(4), 936 (1987).
  9. K. Slinkard and V. L. Singletion, Total phenol analysis : automation and comparison with manual methods, Am. J. Enal. Vitic, 28(1), 49 (1977).
  10. X. P. Zhuang, Y. Y. Lu, and G. S. Yang, Extraction and determination of flavonoid in ginkgo, Chinese. Herbe. Med, 23, 122 (1992).
  11. F. Stirpe and E. D. Corte, The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O), J. Biol. Chem,, 244(14), 3855 (1969). https://doi.org/10.1016/S0021-9258(17)36428-1