Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Effect of Trichoderma sp. GL02 on alleviating Drought Stress in Pepper Plants

Trichoderma sp. GL02에 의한 고추 식물의 건조 스트레스 완화 효과

  • 김상태 (국립농업과학원 농업미생물과) ;
  • 유성제 (국립농업과학원 농업미생물과) ;
  • 송재경 (국립농업과학원 농업미생물과) ;
  • 원항연 (국립농업과학원 농업미생물과) ;
  • 상미경 (국립농업과학원 농업미생물과)
  • Received : 2020.03.27
  • Accepted : 2020.06.02
  • Published : 2020.08.31
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Abstract

Drought stress is one of major environmental stresses in plants; this leads to reduce plant growth and crop yield. In this study, we selected fungal isolate for mitigating drought stress in pepper plants. To do this, 41 fungi were isolated from rhizosphere or bulk soils of various plants in Jeju, Gangneung, Hampyeong in Korea. Out of 41 isolates, we screened two isolates without phytotoxicity through seed germination of tomato, pepper, and cabbage treated with fungal spores; through following plant assay, we selected GL02 as a candidate for alleviating drought stress in pepper plants. As a result of greenhouse test of pepper plants in drought condition, the stomatal conductance on leaves of pepper plants treated with GL02 was increased, whereras the malondialdehyde (MDA) and electrolyte leakage were decreased compared to that in control plants. When stressed plants were rewatered, stomatal conductance of the plants treated with GL02 was increased; the electrolyte leakage was decreased. Based on internal transcribed spacer (ITS) sequencing analysis, isolate GL02 was belonging to genus Trichoderma. Taken together, drought stress in pepper plants treated with GL02 was alleviated, when it was rewatered after drought-stressed, the plants could be recovered effectively. Therefore, Trichoderma sp. GL02 could be used as a bio-fertilizer to alleviate drought stress in pepper plants.

이상기후로 인한 건조 스트레스에 의해 식물의 기공전도도와 광합성의 감소로 생장과 작물생산량이 감소하는 피해가 발생하고 있다. 이러한 식물의 스트레스를 완화하는 유용 곰팡이를 선발하기 위해 국내 농경지에서 41 균주를 분리하였으며, 발아율 검사로 2종의 곰팡이를 선발하였으며, 식물검정을 통해 최종 Trichoderma sp. GL02를 선발하였다. GL02를 고추 식물체에 처리하였을 때, 대조구에 비해 기공전도도가 증가하고, MDA와 전기전도도는 감소하는 것으로 보아, GL02를 처리한 고추 식물체는 건조 스트레스가 완화되며, 건조 후 재관수하였을 때 회복도 효과적이었다. 이러한 결과는 GL02는 고추 식물체에 건조 스트레스를 완화시키는 유용한 소재로 사용될 수 있음을 시사한다. 또한, 추후 포장 실험을 통하여 Trichoderma sp. GL02의 건조 스트레스 완화 효과가 고추 생산량과 연관이 있는지 확인해 볼 예정이다.

Keywords

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