Browse > Article
http://dx.doi.org/10.11625/KJOA.2020.28.3.417

Effect of Trichoderma sp. GL02 on alleviating Drought Stress in Pepper Plants  

Kim, Sang Tae (국립농업과학원 농업미생물과)
Yoo, Sung-Je (국립농업과학원 농업미생물과)
Song, Jaekyeong (국립농업과학원 농업미생물과)
Weon, Hang-Yeon (국립농업과학원 농업미생물과)
Sang, Mee Kyung (국립농업과학원 농업미생물과)
Publication Information
Korean Journal of Organic Agriculture / v.28, no.3, 2020 , pp. 417-430 More about this Journal
Abstract
Drought stress is one of major environmental stresses in plants; this leads to reduce plant growth and crop yield. In this study, we selected fungal isolate for mitigating drought stress in pepper plants. To do this, 41 fungi were isolated from rhizosphere or bulk soils of various plants in Jeju, Gangneung, Hampyeong in Korea. Out of 41 isolates, we screened two isolates without phytotoxicity through seed germination of tomato, pepper, and cabbage treated with fungal spores; through following plant assay, we selected GL02 as a candidate for alleviating drought stress in pepper plants. As a result of greenhouse test of pepper plants in drought condition, the stomatal conductance on leaves of pepper plants treated with GL02 was increased, whereras the malondialdehyde (MDA) and electrolyte leakage were decreased compared to that in control plants. When stressed plants were rewatered, stomatal conductance of the plants treated with GL02 was increased; the electrolyte leakage was decreased. Based on internal transcribed spacer (ITS) sequencing analysis, isolate GL02 was belonging to genus Trichoderma. Taken together, drought stress in pepper plants treated with GL02 was alleviated, when it was rewatered after drought-stressed, the plants could be recovered effectively. Therefore, Trichoderma sp. GL02 could be used as a bio-fertilizer to alleviate drought stress in pepper plants.
Keywords
chili pepper; drought; fungi;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Anjum, S. A., L. C. Wang, M. Farooq, M. Hussain, L. L. Xue, and C. M. Zou. 2011. Brassinolide application improves the drought tolerance in maize through modulation of enzymatic antioxidants and leaf gas exchange. J. Afr. Agric. Res. 6: 2026-2032.
2 Assefa, Y., S. A. Staggenborg, and P. V. V. Prasad. 2010. Grain sorghum water requirement and responses to drought stress: A review. Crop Management. doi:10.1094/CM-20101-1109-01-RV.
3 Bang, N. K., W. H. Nam, E. M. Hong, M. J. Hayes, and M. D. Svoboda. 2018. Assessment of the meteorological characteristics and statistical drought frequency for the extreme 2017 spring drought event across South Korea. Journal of the Korean Society of Agricultural Engineers. 60: 37-48 (in Korean).
4 Bao, A. K., S. M. Wang, G. Q. Wu, J. J. Xi, J. L. Zhang, and C. U. Wang. 2009. Overexpression of the Arabidopsis H+-PPase enhanced the salt and drought tolerance in transgenic alfalfa (Medicago sativa L.). Plant Sci. 176: 232-240.   DOI
5 Barrs, H. D. and P. E. Weatherley. 1962. A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aus. J. Biol. Sci. 15: 413-428.   DOI
6 Chen, W., X. Yao, K. Cai, and J. Chen. 2011. Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption. Biol Trace Elem Res. 142: 67-76.   DOI
7 Chomkitichai, W., A. Chumyam, P. Rachtanapun, J. Uthaibutra, and K. Saengnil. 2014. Reduction of reactive oxygen species production and membrane damage during storage of 'Daw' longan fruit by chlorine dioxide. Sci. Hortic. 170: 143-149.   DOI
8 Gusain, Y. S., U. S. Singh, and A. K. Sharma. 2014. Enhance activity of stress related enzymes in rice (Oryza sativa L.) induced by plant growth promoting fungi under drought stress. Afr. J. Agric. Res. 9: 1430-1434.   DOI
9 Guler, N. S., N. Pehlivan, S. A. Karaoglu, S. Guzel, and A. Bozdeveci. 2016. Trichoderma atroviride ID20G inoculation ameliorates drought stress-induced damages by improving antioxidant defence in maize seedlings. Acta Physiol Plant 38: 132.   DOI
10 Hasanuzzaman, M., K. Nahar, S. S. Gill, and M. Fujita. 2014. Drought stress responses in plants, oxidative stress, and antioxidant defense. In: Tuteja, N., Gill, S. S. (Eds.), Climate Change and Plant Abiotic Stress Tolerance, first edition. pp. 209-210. Wiley-VCH Verlag GmbH & Co. KGaA.
11 Hashem, A., E. F. Abd_Allah, A. A. Alqarawi, A. A. A. Huqail, and D. Egamberdieva. 2014. Alleviation of abiotic stress in Ochradenus baccatus (Del.) by Trichoderma hamatum (Bonord.) Bainier. J Plant Interact. 9: 857-868.   DOI
12 Mastouri, F., T. Bjorkman, and G. E. Harman. 2010. Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedling. Phytopathology 100: 1213-1221.   DOI
13 Kim, S. T., S. J. Yoo, J. Song, H. Y. Weon, and M. K. Sang. 2019. Screening of bacterial strains for alleviating drought stress in chili pepper plants. Res. Plant Dis. 25: 1-7.   DOI
14 Kusvuran, S. 2012. Effects of drought and salt stresses on growth, stomatal conductance, leaf water osmotic potentials of melon genotypes (Cucumismelo L.). Afr. J. Agric. Res. 7, 775-781.
15 Lin, Y., D. B. Watte, J. W. Kloepper, Y. Feng, and H. A. Torbert. 2020. Influence of plant growth-promoting rhizobacteria on corn growth under drought stress Commun Soil Sci Plant Anal. 51: 250-264.   DOI
16 Matiu, M., D. P. Ankerst, and A. Menzel. 2017. Interactions between temperature and drought in global and regional crop yield variability during 1961-2014. PLOS ONE. 12: e0178339.   DOI
17 Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405-410.   DOI
18 Miller, G., N. Suzuki, L. Rizhsky, A. Hegie, S. Koussevitzky, and R. Mittler. 2007. Double mutants deficient in cytosolic and thylakoid ascorbate peroxidase reveal a complex mode of interaction between reactive oxygen species, plant development, and response to abiotic stresses. Plant Physiol. 144: 1777-1785.   DOI
19 Mona, S. A., A. Hashem, E. F. Abd_Allah, A. A. Alqarawi, D. W. K. Soliman, S. Wirth, and D. Egamberdieva. 2017. Increased resistance of drought by Trichoderma harzianum fungal treatment correlates with increased secondary metabolites and proline content. J. Integr. Agric. 16: 1751-1757.   DOI
20 Racic, G., I. Vukelic, L. Prokic, N. Curcic, M. Zoric, L. Jovanovic, and D. Pankovic. 2018. The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato. Ann Appl Biol. 173: 213-221.   DOI
21 Salam, E. A., A. Alatar, and M. A. El-Sheikh. 2017. Inoculation with arbuscular mycorrhizal fungi alleviates harmful effects of drought stress on damask rose. Saudi J. Biol. Sci. 25: 1772-1780.   DOI
22 Tiwari, S., C. Lata, P. S. Chauhan, and C. S. Nautiyal. 2016. Pseudomonas putida attunes morphophysiological, biochemical and molecular responses in Cicer arietinum L. during drought stress and recovery. Plant Physiol. Biochem. 99: 108-117.   DOI
23 Xie, Z., Y. Chu, W. Zhang, D. Lang, and X. Zhang. 2019. Bacillus pumilus alleviates drought stress and increases metabolite accumulation in Glycyrrhiza uralensis Fisch. Environ Exp Bot 158: 99-106.   DOI
24 Yang, T., S. Ma, and C. C. Dai. 2014. Drought degree constrains the beneficial effects of a fungal endophyte on Atractylodes lancea. J. Appl. Microbiol. 117: 1435-1449.   DOI
25 Yoo, S. J., D. J. Shin, H. Y. Weon, J. Song, and M. K. Sang. 2018. Aspergillus terreus JF27 promotes the growth of tomato plants and induces resistance against Pseudomonas syringae pv. tomato. Mycobiology 46: 147-153.   DOI