환경영향평가 (Journal of Environmental Impact Assessment)

한국환경영향평가학회 (Korean Society of Environmental Impact Assessment)
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비침습 샘플 DNA 분석으로 유추한 영월 한반도습지 내 멧돼지(Sus scrofa)의 생태 연구

Ecological Studies of Wild boars(Sus scrofa) in Yeongwol Hanbando Wetland Inferred through DNA Analysis of Non-invasive Samples

  • 김민경 (서울대학교 생명과학부) ;
  • 이상임 (대구경북과학기술원 뉴바이올로지전공) ;
  • 박효민 (이화여자대학교 환경공학과) ;
  • 이상돈 (이화여자대학교 환경공학과)
  • Kim, Minkyung (School of Biological Sciences, Seoul National University) ;
  • Lee, Sang-im (Department of New Biology, DGIST) ;
  • Park, Hyomin (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Lee, Sangdon (Department of Environmental Science and Engineering, Ewha Womans University)
  • 투고 : 2020.04.24
  • 심사 : 2020.05.27
  • 발행 : 2020.06.30
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초록

본 연구는 비침습 샘플인 털을 이용하여 영월 한반도습지 내 서식하는 멧돼지(Sus scrofa)의 유전분석을 통하여 그들의 서식 생태를 유추하였다. 털 시료는 2018년 11월부터 2019년 5월까지 한반도습지(2.772㎢) 내에서 비빔목 및 헤어트랩을 이용하여 수집하였다. 털 시료로부터 DNA를 추출하여 개체의 종과 성을 PCR을 통해 파악하였으며 6개의 마이크로새틀라이트 마커를 이용하여 개체 구분과 개체 간 유전적 근연관계를 유추하였다. 수집된 털 시료 중 총 16개의 털이 멧돼지의 시료였으며, 이는 암컷 7마리, 수컷 3마리의 10개체로부터 수집된 것임이 판명되었다. 이 10개체의 유전적 관계를 추정해 본 결과, 이들이 만들어내는 45쌍 중에 9쌍의 개체가 친족관계일 가능성이 높게 나타났다. 개체 쌍의 친족관계와 털 시료가 채집된 위치를 함께 고려하여 본 결과, 한반도습지 일대에서 서식하는 멧돼지는 암컷과 그 자손으로써 모계 가족단위로 생활하는 것으로 추정되며, 이는 기존에 알려진 멧돼지의 습성과도 일치하는 결과이다. 그러나 본 연구에 사용된 샘플과 마이크로새틀라이트 마커의 수가 제한적이므로 향후 추가적인 분석이 필요하다.

This study inferred the ecology of habitat use of the wild boars (Sus scrofa) in Yeongwol Hanbando wetland through DNA analysis using non-invasive samples of hairs. From November 2018 to May 2019, hair samples were collected from rubbing trees and hairtraps within the Hanbando wetland (2.772 ㎢). We extracted DNA from the hair samples and conducted PCR to verify the species and identify sex of the individuals. In addition we analysed 6 microsatellite markers to identify individuals and genetic relationship among the pairs of individuals. A total of 16 boar hairs were sampled, which turned out to be from 10 individual (7 females and 3 males) boars. We found that 9 pairs, out of 45 possible pairs, were most likely to be relatives. The result from kinship data and the location of the sampled hairs suggest that wild boars in this area live as family groups that consist of a mother and her offspring, which is consistent with known habits of wild boars. It is needed to include more samples and microsatellite markers for better precise estimation of kinship among the boar individuals.

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