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http://dx.doi.org/10.14249/eia.2020.29.3.230

Ecological Studies of Wild boars(Sus scrofa) in Yeongwol Hanbando Wetland Inferred through DNA Analysis of Non-invasive Samples  

Kim, Minkyung (School of Biological Sciences, Seoul National University)
Lee, Sang-im (Department of New Biology, DGIST)
Park, Hyomin (Department of Environmental Science and Engineering, Ewha Womans University)
Lee, Sangdon (Department of Environmental Science and Engineering, Ewha Womans University)
Publication Information
Journal of Environmental Impact Assessment / v.29, no.3, 2020 , pp. 230-238 More about this Journal
Abstract
This study inferred the ecology of habitat use of the wild boars (Sus scrofa) in Yeongwol Hanbando wetland through DNA analysis using non-invasive samples of hairs. From November 2018 to May 2019, hair samples were collected from rubbing trees and hairtraps within the Hanbando wetland (2.772 ㎢). We extracted DNA from the hair samples and conducted PCR to verify the species and identify sex of the individuals. In addition we analysed 6 microsatellite markers to identify individuals and genetic relationship among the pairs of individuals. A total of 16 boar hairs were sampled, which turned out to be from 10 individual (7 females and 3 males) boars. We found that 9 pairs, out of 45 possible pairs, were most likely to be relatives. The result from kinship data and the location of the sampled hairs suggest that wild boars in this area live as family groups that consist of a mother and her offspring, which is consistent with known habits of wild boars. It is needed to include more samples and microsatellite markers for better precise estimation of kinship among the boar individuals.
Keywords
wild boar; hanbando wetland; non-invasive sample; hair; genetic relationship;
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