대한한의학방제학회지 (Herbal Formula Science)

  • 제28권2호
  • /
  • Pages.147-155
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  • 2020
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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천심련(穿心蓮) 메탄올 추출물의 항염증효과

Anti-inflammatory Effects of Andrographis Herba MeOH Extract on LPS-induced Raw 264.7 Cells

  • 정지윤 (대구한의대학교 한의과대학) ;
  • 박정아 (대구한의대학교 한의과대학)
  • Jung, Ji Yun (College of Korean Medicine, Daegu Haany University) ;
  • Park, Chung A (College of Korean Medicine, Daegu Haany University)
  • 투고 : 2020.05.01
  • 심사 : 2020.05.25
  • 발행 : 2020.05.31
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초록

PURPOSE : Andrographis Herba is used as a traditional herbal medicine in the Asian countries for the treatment common cold, fever, diabetes, hypertension, hepatitis, skin infections, snake bite, and other diseases. In this study, we investigated the anti-inflammatory effect of MeOH extract of Andrographis Herba (AHME) on LPS-activated Raw 264.7 cells. METHODS : Cell viability was determined by MTT assay. Nitric oxide (NO) production was determined by Griess reagent. Pro-inflammatory cytokines were detected by enzyme-linked immunosorbent assay. Expression levels of pro-inflammatory proteins were determined by Western blot analysis. RESULTS : Production of NO in LPS activated Raw 264.7 cells, was significantly decreased by pre-treatment with 3-30 ㎍/mL of AHME. Production of pro-inflammatory mediators such as TNF-α and IL were significantly decreased by AHME 30 ㎍/mL pre-treatment. AHME significantly decreased p-IκB and NF-κB expression. CONCLUSION : The results of this study indicate that AHME could inhibit the acute inflammatory response, via modulation of NF-κB activation.

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