DOI QR코드

DOI QR Code

번행초 추출물의 항산화 및 항염증 효과

Antioxidant and Anti-inflammatory Activities of Tetragonia tetragonoides Water Extract

  • 공지현 (부산대학교 한의학전문대학원) ;
  • 이기연 (부산대학교 한의학전문대학원) ;
  • 노대현 (부산대학교 한의학전문대학원) ;
  • 이장천 (부산대학교 한의학전문대학원) ;
  • 안원근 (부산대학교 한의학전문대학원) ;
  • 이부균 (부산대학교 한의학전문대학원)
  • Kong, Ji Hyun (School of Korean Medicine, Pusan National University) ;
  • Lee, Ki Yeon (School of Korean Medicine, Pusan National University) ;
  • Rho, Dae Hyun (School of Korean Medicine, Pusan National University) ;
  • Lee, Jang-Cheon (School of Korean Medicine, Pusan National University) ;
  • An, Won Gun (School of Korean Medicine, Pusan National University) ;
  • Lee, Boo-Kyun (School of Korean Medicine, Pusan National University)
  • 투고 : 2020.04.27
  • 심사 : 2020.05.25
  • 발행 : 2020.05.31

초록

Objectives : Tetragonia tetragonioides is one of the traditional herbal medicines that can be used to protect the stomach and treat cancer. However, its mechanism of overcoming gastrointestinal disorders is unclear. In this study, we investigated antioxidant and anti-inflammatory effects of Tetragonia tetragonioides Water Extract (TTWE) on RAW264.7 cells. Methods : The cell viability by TTWE was measured by using MTT assay. The free radical scavenging ability and cytokine production were analyzed by using ELISA Kit. SPSS version 25 was used for statistical analysis. Results : According to the results of this study, the cell viability measurement of TTWE significantly affected the cell viability. The radical scavenging ability of TTWE showed the highest effect compared to the positive control group when the concentration was 3.1-12.5 ㎍/ml, and significantly inhibited NO production induced by LPS. In addition, the inhibitory effect of TTWE on the production of IL-6 and TNF-α induced by LPS was significant at both TTWE concentrations of 12.5 ㎍/ml [p <0.01 (IL-6), p <0.05 (TNF-α)]. Conclusion : In conclusion, it is suggested that the antioxidant function of Tetragonia tetragonioides Water Extract could be used to prevent and treat inflammatory diseases.

키워드

참고문헌

  1. NH Kim, Pro-inflammatory inhibition effect of Ferulic acid isolated from Tetragonia tetragonoides. Kyungpook National University thesis dissertation, 2016.
  2. L Chen, Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2018;9:7204-7218. https://doi.org/10.18632/oncotarget.23208
  3. C Gabay, Interleukin-6 and chronic inflammation. Arthritis Res. Ther. 2006;8:53. https://doi.org/10.1186/ar1914
  4. P Fang, Immune cell subset differentiation and tissue inflammation. Journal of Hematology & Oncology. 2018;11(1):97. https://doi.org/10.1186/s13045-018-0637-x
  5. SL Masters, A Simon, I Aksentijevich, DL Kastner, Horror autoinflammaticus: the molecular pathophysiology of autoinflammatory disease. Ann. Rev. Immunol. 2009;27:621-668. https://doi.org/10.1146/annurev.immunol.25.022106.141627
  6. RP Tracy, The Five Cardinal Signs of Inflammation. The Journals of Gerontology: Series A. 2006;61(10):1051-1052. https://doi.org/10.1093/gerona/61.10.1051
  7. IH Kim, CH Choi, SW Lee, YS Song, Antiinflammatory Effect of Mugi-hwan Water Extract in RAW 264.7 Cells. Journal of Korean Medicine Rehabilitation. 2013;23(3);27-35.
  8. SH Lee, YH Choi, Link between Periodontal Disease and Cancer: A Recent Research Trend, Journal of Life Science. 2013;23(4):602-608. https://doi.org/10.5352/JLS.2013.23.4.602
  9. Rostom A, Dube C, Wells G, Tugwell P, Welch V, Jolicoeur E, McGowan J. Prevention of NSAID-induced gastroduodenal ulcers. Cochrane Database Syst Rev. 2002;4:DC002296.
  10. BJ Kim, Glucocorticoid and Pain Treatment, The Journals of critical pain. 2002;1(2):63-69.
  11. DC Wallace, Science.Mitochondrial genetics: A paradigm for aging and degenerative diseases. Science. 1992;256:628-632. https://doi.org/10.1126/science.1533953
  12. S Loft, A Astrup, B Buemann, HE Poulsen, H.E. Oxidative DNA damage correlates with oxygen consumption in humans. FASEB J. 1994;8:534-537. https://doi.org/10.1096/fasebj.8.8.8181672
  13. S Duffy, BA MacViar, In vitro ischemia promotes calcium influx and intracellular calcium release in hippocampal astrocytes. J Neurosci. 1996;16(1):71-81. https://doi.org/10.1523/JNEUROSCI.16-01-00071.1996
  14. Al Harabin, JC Braisted, ET Flynn, Response of antioxidant enzymes to intermitent and continuous hyperbaric oxygen. J Appl physiol. 1990;69:328-335. https://doi.org/10.1152/jappl.1990.69.1.328
  15. JM Jacobson , JR Michael, MH JR Jafri, GH Gurtner, Antioxidants and antioxidant enzymes protect against pulmonary oxygen toxicity in the rabbit. J Appl Physiol 1990;68: 1252-1259. https://doi.org/10.1152/jappl.1990.68.3.1252
  16. JK J, Healthy native grass. Nexus Books, 2009:267.
  17. T Aoki, K Takagi, T. Hira, Two naturally occurring acyclic diterpene and norditerpene aldehydes from Tetragonia tetragonoides. Phytochemistry. 1982;21(6):1361-1363. https://doi.org/10.1016/0031-9422(82)80142-8
  18. M Kato, T Takeda, Y Ogihara, Studies on the structure of polysaccharide from Tetragonia tetragonoides. I. Chem. Pharm. Bull. 1985;33(9):3675-3680. https://doi.org/10.1248/cpb.33.3675
  19. HY Ryu, KH Bae, EJ Kim, SJ Park, BH Lee, 2007, Evaluation for the Antimicrobial, Antioxidant and Antithrombosis Activity of Natural Spices for Fresh-cut yam. J. Life Sci, 17(5): 652-657. https://doi.org/10.5352/JLS.2007.17.5.652
  20. E Okuyama, M Yamazaki, The principles of Tetragonia tetragonoides having antiulcerogenic activity. II. Isolation and structure of cerebrosides. Isolation and identification of a sterol glucoside mixture (compound A). Yakuguku zasshi 1983;31(7):43-48. https://doi.org/10.1248/yakushi1947.103.1_43
  21. JH Kim, CS Seo, WYJeon, HK Shin, The Compositional Differences of Sipjeondaebotang( Siquandabu-tang), Decoctions Extracted by Different Extraction Method and Extraction Time. The Journal of Oriental Obstetrics & Gynecology. 2012;25(2):108-119.
  22. GY Choi, Chinese Herb. Vol.2:Shanghai Science and Technology Press, 1999:752-3.
  23. JC Lee, Effect of the Solvent Extracts from Tetragonia tetragonioides on the Activity of Tumor and Anti-inflammatory Cells in Mice. Graduate School of WonKwang Univ. 1991.
  24. SI Kodato, M Nakagawa, T Hino, Synthesis of cerebroside B1b with antiulcerogenic activity II. Total synthesis and determination of absolute configuration of cerebroside B1b and its stereoisomers. Tetrahedron. 1989;45(23):7263-7280. https://doi.org/10.1016/S0040-4020(01)89187-0
  25. YG Park, YH Nam, Studies on the Antioxidative effects of Tetragonia tetragonioides (Pall.) O. KUNTZE. 1983;17(2):191-191.
  26. JB Jo, EH Lee and YJ Jo, Beauty Food Activities of Isolated Phenolic Compounds from Tetragonia tetragonioides. The Korean Society of Food Science and Nutrition. 2016:333-341.
  27. EY Ko, SH Cho, KP Kang, GB Kim, JH Lee, YJ Jeon, DK Kim, GA Ahn, KN Kim, Anti-inflammatory activity of hydrosols from Tetragonia tetragonoides in LPS- induced RAW 264.7 cells. EXCLI J. 2017;16:521-530. https://doi.org/10.17179/excli2017-121
  28. HJ Choi, ST Yee and WH Joo, Antidiabetic Activity of Polysaccharide Extract from Tetragonia tetragonoides in Streptozotocininduced Diabetic Mice. Journal of Life Science 2017;27(5:)579-583. https://doi.org/10.5352/JLS.2017.27.5.579
  29. OS Aslanturk, Genotoxicity: A Predictable Risk to Our Actual World. BoD - Books on Demand. 2018:4.
  30. V Bondet, W Brand-Williams, C Berset. Kinetics and Mechanisms of Antioxidant Activity using the DPPH Free Radical Method. Lebensm.-Wiss. u.-Technol. 1997;30:609-615. https://doi.org/10.1006/fstl.1997.0240
  31. P Molyneux. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Schi. Technol., 2004;26:211-219.
  32. JN Sharma, A Al-Omran, SS Pavathy. Role of nitric oxide in inflammatory diesease. 2007;15(6):252-259. https://doi.org/10.1007/s10787-007-0013-x
  33. HJ Koo. Inhibition of iNOS mRNA expression and NO production by LED light irradiation. Nambu University. School of Health and Welfare. 2010.
  34. B Beutler, A Cerami. The biology of cachectin/$TNF-{\alpha}$ primary mediator of the host response. Annu. Rev. Immunol. 1989;7:625-655. https://doi.org/10.1146/annurev.iy.07.040189.003205
  35. M Guslandi. Nitric oxide and inflammatory bowel diseases. Eur. J. Clin. Invest. 1998; 28:904-907. https://doi.org/10.1046/j.1365-2362.1998.00377.x
  36. AV Delgado, AT McManus, JP Chambers. Production of Tumor Necrosis Factor-alpha, Interleukin 1-beta, Interleukin 2, and Interleukin 6 by rat leukocyte sub- populations after exposure to Substance P. Neuropeptides. 2003;37:355-361. https://doi.org/10.1016/j.npep.2003.09.005
  37. SJ Kim, HJ Jeong, PD Moon, KM Lee, HB Lee, HJ Jung, SK Jung, HK Rhee, DC Yang, SH Hong, HM Kim. Anti-inflammatoryactivity of Gumiganghwaltang through the inhibition of nuclear factor-kap- pa B activation in peritoneal macrophages. Biol. Pharm. Bull. 2005;28:233-237. https://doi.org/10.1248/bpb.28.233
  38. K. Sheeja, P. K. Shihab, G. Kuttan. Antioxidant and anti-inflammatory activities of the plant Andrographis Paniculata Nees. Immunopharmacology and immunotoxicology, 2006; 28:129-140. https://doi.org/10.1080/08923970600626007