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http://dx.doi.org/10.14374/HFS.2020.28.2.147

Anti-inflammatory Effects of Andrographis Herba MeOH Extract on LPS-induced Raw 264.7 Cells  

Jung, Ji Yun (College of Korean Medicine, Daegu Haany University)
Park, Chung A (College of Korean Medicine, Daegu Haany University)
Publication Information
Herbal Formula Science / v.28, no.2, 2020 , pp. 147-155 More about this Journal
Abstract
PURPOSE : Andrographis Herba is used as a traditional herbal medicine in the Asian countries for the treatment common cold, fever, diabetes, hypertension, hepatitis, skin infections, snake bite, and other diseases. In this study, we investigated the anti-inflammatory effect of MeOH extract of Andrographis Herba (AHME) on LPS-activated Raw 264.7 cells. METHODS : Cell viability was determined by MTT assay. Nitric oxide (NO) production was determined by Griess reagent. Pro-inflammatory cytokines were detected by enzyme-linked immunosorbent assay. Expression levels of pro-inflammatory proteins were determined by Western blot analysis. RESULTS : Production of NO in LPS activated Raw 264.7 cells, was significantly decreased by pre-treatment with 3-30 ㎍/mL of AHME. Production of pro-inflammatory mediators such as TNF-α and IL were significantly decreased by AHME 30 ㎍/mL pre-treatment. AHME significantly decreased p-IκB and NF-κB expression. CONCLUSION : The results of this study indicate that AHME could inhibit the acute inflammatory response, via modulation of NF-κB activation.
Keywords
Andrographis Herba; inflammation; $NF-{\kappa}B$;
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