Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Streptomycin Resistant Isolates of Pseudomonas syringae pv. actinidiae in Korea

국내에서 분리된 Pseudomonas syringae pv. actinidiae 균주들의 스트렙토마이신 저항성

  • Lee, Young Sun (Department of Biology, Sunchon National University) ;
  • Kim, Gyoung Hee (Department of Plant Medicine, Sunchon National University) ;
  • Song, Yu-Rim (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Koh, Young Jin (Department of Plant Medicine, Sunchon National University) ;
  • Jung, Jae Sung (Department of Biology, Sunchon National University)
  • Received : 2020.03.02
  • Accepted : 2020.03.17
  • Published : 2020.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Streptomycin resistant isolates of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker in kiwifruit, were found in Korea. A total of 734 isolates of P. syringae pv. actinidiae collected between 2008 and 2017 from bacterial canker infections in 111 kiwifruit orchards were assessed for streptomycin resistance. The survival of each isolate was screened against 100 ㎍/ml of streptomycin. Among 734 isolates, 38 streptomycin resistant P. syringae pv. actinidiae isolates originated from nine orchards were found. Streptomycin resistant isolates belonging to biovar 2 were found in several individual years, but ones belonging to biovar 3 were found in Korea only since 2016. Therefore, to use streptomycin for control of bacterial canker in kiwifruit orchards should be very careful, and it is necessary to check the streptomycin susceptibility of the pathogen before use in kiwifruit orchards.

국내에서 분리된 키위 궤양병 원인균인 Pseudomonas syringae pv. actinidiae 균주들에서 스트렙토마이신 저항성 균주가 확인되었다. 2008년부터 2017년 사이에 키위 궤양병이 발병한 111개 과수원에서 분리된 734개 균주의 스트렙토마이신 저항성을 조사하였다. 각 균주의 생존 여부는 100 ㎍/ml의 스트렙토마이신 배지에서 검정하였다. 734개 균주들 중에서 9개 과수원에서 분리된 총 38개 P. syringae pv. actinidiae 균주가 저항성을 나타내었다. Biovar 2에 속하는 저항성 균주들의 경우 몇 개의 특정년도에 발견된 반면, biovar 3에 속하는 저항성 균주들은 2016년 이후에만 발견되었다. 따라서, 키위 궤양병 방제용으로 스트렙토마이신 사용 시 주의해야 하며, 만일 사용하려면 사용 전에 항생제 감수성 테스트가 필요하다.

Keywords

References

  1. Cameron, A. and Sarojini, V. 2014. Pseudomonas syringae pv. actinidiae: chemical control, resistance mechanisms and possible alternatives. Plant Pathol. 63: 1-11. https://doi.org/10.1111/ppa.12066
  2. Chapman, J. R., Taylor, R. K., Weir, B. S., Romberg, M. K., Vanneste, J. L., Luck, J. et al. 2012. Phylogenetic relationships among global populations of Pseudomonas syringae pv. actinidiae. Phytopathology 102: 1034-1044. https://doi.org/10.1094/PHYTO-03-12-0064-R
  3. Chiou, C.-S. and Jones, A. L. 1995a. Expression and identification of the strA-strB gene pair from streptomycin-resistant Erwinia amylovora. Gene 152: 47-51. https://doi.org/10.1016/0378-1119(94)00721-4
  4. Chiou, C.-S. and Jones, A. L. 1995b. Molecular analysis of hight-level streptomycin resistance in Erwinia amylovora. Phytopathology 85: 324-328. https://doi.org/10.1094/Phyto-85-324
  5. Forster, H., McGhee, G. C., Sundin, G. W. and Adaskaveg, J. E. 2015. Characterization of streptomycin resistance in isolates of Erwinia amylovora in California. Phytopathology 105: 1302-1310. https://doi.org/10.1094/PHYTO-03-15-0078-R
  6. Han, H. S., Nam, H. Y., Koh, Y. J., Hur, J.-S. and Jung, J. S. 2003. Molecular bases of high-level streptomycin resistance in Pseudomonas marginalis and Pseudomonas syringae pv. actinidiae. J. Microbiol. 41: 16-21.
  7. Kim, G. H., Choi, E. D., Lee, Y. S., Jung, J. S. and Koh, Y. J. 2016a. Spread of bacterial canker of kiwifruit by secondary infection of Pseudomonas syringae pv. actinidiae biovar 3 in Gyeongnam in 2016. Res. Plant Dis. 22: 276-283. (In Korean) https://doi.org/10.5423/RPD.2016.22.4.276
  8. Kim, G. H., Kim, K.-H., Son, K. I., Choi, E. D., Lee, Y. S., Jung, J. S. et al. 2016b. Outbreak and spread of bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae biovar 3 in Korea. Plant Pathol. J. 32: 542-551.
  9. Koh, Y. J., Cha, B. J., Chung, H. J. and Lee, D. H. 1994. Outbreak and spread of bacterial canker in kiwifruit. Korean J. Plant Pathol. 10: 68-72.
  10. Koh, Y. J., Kim, G. H. and Jung, J. S. 2017. A proposed manual for the efficient management of kiwifruit bacterial canker in Korea. Res. Plant Dis. 23: 1-18. (In Korean) https://doi.org/10.5423/RPD.2017.23.1.1
  11. Koh, Y. J., Kim, G. H., Koh, H. S., Lee, Y. S., Kim, S.-C. and Jung, J. S. 2012. Occurrence of a new type of Pseudomonas syringae pv. actinidiae strain of bacterial canker on kiwifruit in Korea. Plant Pathol. J. 28: 423-427. https://doi.org/10.5423/PPJ.NT.05.2012.0061
  12. Lee, J. H., Kim, J. H., Kim, G. H., Jung, J. S., Hur, J.-S. and Koh, Y. J. 2005. Comparative analysis of Korean and Japanese strains of Pseudomonas syringae pv. actinidiae causing bacterial canker of kiwifruit. Plant Pathol. J. 21: 119-126. https://doi.org/10.5423/PPJ.2005.21.2.119
  13. Lee, Y. S., Kim, J., Kim, G. H., Choi, E. D., Koh, Y. J. and Jung, J. S. 2017. Biovars of Pseudomonas syringae pv. actinidiae strains, the causal agent of bacterial canker of kiwifruit, isolated in Korea. Res. Plant Dis. 23: 35-41. (In Korean) https://doi.org/10.5423/RPD.2017.23.1.35
  14. McCann, H. C., Li, L., Liu, Y., Li, D., Pan, H., Zhong, C. et al. 2017. Origin and evolution of the kiwifruit canker pandemic. Genome Biol. Evol. 9: 932-944. https://doi.org/10.1093/gbe/evx055
  15. Nakajima, M., Yamashita, S., Takikawa, Y., Tsuyumu, S., Hibi, T. and Goto, M. 1995. Similarity of streptomycin resistance gene(s) in Pseudomonas syringae pv. actinidiae with strA and strB of plasmid RSF 1010. Ann. Phytopathol. Soc. Jpn. 61: 489-492. https://doi.org/10.3186/jjphytopath.61.489
  16. Sawada, H., Kondo, K. and Nakaune, R. 2016. Novel biovar (biovar 6) of Pseudomonas syringae pv. actinidiae causing bacterial canker of kiwifruit (Actinidia deliciosa) in Japan. Jpn. J. Phytopathol. 82: 101-115. https://doi.org/10.3186/jjphytopath.82.101
  17. Sawada, H., Miyoshi, T. and Ide, Y. 2014. Novel MLSA group (Psa5) of Pseudomonas syringae pv. actinidiae causing bacterial canker of kiwifruit (Actinidia chinensis) in Japan. Jpn. J. Phytopathol. 80: 171-184. https://doi.org/10.3186/jjphytopath.80.171
  18. Scortichini, M. 1994. Occurrence of Pseudomonas syringae pv. actinidiae on kiwifruit in Italy. Plant Pathol. 43: 1035-1038. https://doi.org/10.1111/j.1365-3059.1994.tb01654.x
  19. Scotichini, M., Marcelletti, S., Ferrante, P., Petriccione, M. and Firrao, G. 2012. Pseudomonas syringae pv. actinidiae: a re-emerging, multi-faceted, pandemic pathogen. Mol. Plant Pathol. 13: 631-640. https://doi.org/10.1111/j.1364-3703.2012.00788.x
  20. Takikawa, Y., Serizawa, S., Ichikawa, T., Tsuyumu, S. and Goto, M. 1989. Pseudomonas syringae pv. actinidiae pv. nov.: the causal bacterium of kiwifruit canker in Japan. Ann. Phytopathol. Soc. Jpn. 55: 437-444. https://doi.org/10.3186/jjphytopath.55.437