Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

First Report of Peach Fruit Rot Caused by Fusarium avenaceum in Korea

Fusarium avenaceum에 의한 복숭아 신규 과실 썩음병 발생 보고

  • Heo, A Yeong (Department of Plant Medicals, Andong National University) ;
  • Koo, Young Mo (Department of Plant Medicals, Andong National University) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University) ;
  • Kim, Sang Hee (Division of Applied Life Science (BK 21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Chung, Gyu Young (Division of Horticulture & Medicinal Plant, Andong National University) ;
  • Choi, Hyong Woo (Department of Plant Medicals, Andong National University)
  • 허아영 (국립안동대학교 생명과학대학 식물의학과) ;
  • 구영모 (국립안동대학교 생명과학대학 식물의학과) ;
  • 최영준 (국립군산대학교 자연과학대학 생물학과) ;
  • 김상희 (경상대학교 생명과학부) ;
  • 정규영 (국립안동대학교 생명과학대학 원예.생약융합학부) ;
  • 최형우 (국립안동대학교 생명과학대학 식물의학과)
  • Received : 2020.02.18
  • Accepted : 2020.03.28
  • Published : 2020.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

In July 2019, typical rot symptom was observed on peach fruits harvested from the fields at Andong, Korea. As the disease progressed, white and purple colored mycelial mat developed on the surface of the infected fruits. A causal pathogen was isolated from the infected fruit and cultured on potato dextrose agar media for identification. Fungal colonies on potato dextrose agar produced 3 pigments, including purple, yellow, and white colors. The isolate incited fruit rot symptoms on artificially inoculated peach fruits, from which the same fungus was isolated, fulfilling Koch's postulates. Based on the morphological characteristics and sequence analysis of rDNA internal transcribed spacer, translation elongation factor 1-alpha, and β-tubulin, the causal agent of the disease was identified as Fusarium avenaceum. This study is the first report of fruit rot of peach fruits caused by Fusarium avenaceum in Korea.

2019년 7월 안동지역 노지 재배지에서 복숭아에 과실 썩음병 피해가 발생하였다. 피해 과실에서는 병이 진전됨에 따라, 썩음증상과 함께, 흰색과 자주색을 띄는 균사 및 포자가 관찰되었다. 병원균을 순수 분리한 뒤, 건전한 복숭아 과일에 접종하였을 때 동일한 과실 썩음 증상을 유도하였다. 분리된 병원균의 internal transcribed spacer (ITS), translation elongation factor 1-alpha (TEF) 그리고 β-tubulin (β-TUB) sequence 분석을 통해 Fusarium avenaceum으로 동정되었다. 따라서, 이 증상을 Fusarium avenaceum에 의한 "복숭아 과실 썩음병"으로 명명하고자 한다. 분리된 균주는 농촌진흥청 국립농업과학원 미생물 은행[Korean Agricultural Culture Collection(KACC)]에 기탁되었다(KACC accession number 48936).

Keywords

References

  1. Aktaruzzaman, M., Afroz, T., Lee, Y.-G. and Kim, B.-S. 2018. Morpho-logical and molecular characterization of Fusarium tricinctum causing postharvest fruit rot of pumpkin in Korea. J. Gen. Plant Pathol. 84: 407-413. https://doi.org/10.1007/s10327-018-0803-6
  2. Arrebola, E., Sivakumar, D., Bacigalupo, R. and Korsten, L. 2010. Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases. Crop Prot. 29: 369-377. https://doi.org/10.1016/j.cropro.2009.08.001
  3. Bak, S., Seo, E., Kim, S. Y., Park, W. H. and Lee, S.-H. 2017. First report of Apricot pseudo-chlorotic leaf spot virus infecting peach trees in South Korea. Res. Plant Dis. 23: 75-81. (In Korean) https://doi.org/10.5423/RPD.2017.23.1.75
  4. Choi, J.-H., Nah, J.-Y., Jin, H.-S., Lim, S.-B., Paek, J.-S., Lee, M.-J. et al. 2019. Identification and chemotype profiling of Fusarium species in Korean oat. Res. Plant Dis. 25: 157-163. (In Korean) https://doi.org/10.5423/RPD.2019.25.4.157
  5. Choi, I.-Y., Kim, J., Ju, H.-J., Park. J.-H. and Shin H.-D. 2015. First report of Fusarium wilt caused by Fusarium oxysporum on Kohlrabi in Korea. Res. Plant Dis. 21: 27-31. (In Korean) https://doi.org/10.5423/RPD.2015.21.1.027
  6. Kim, J.-H., Kim, J., Choi, I.-Y., Cheong, S.-S., Uhm, M.-J. and Lee, W. H. 2015. First report of Anthracnose on bitter gourd caused by Colletotrichum gloeosporioides in Korea. Res. Plant Dis. 21: 32-35. (In Korean) https://doi.org/10.5423/RPD.2015.21.1.032
  7. Kim, Y. S., Yun, Y. J. and Jeon. Y. 2018. First report of black rot caused by Diplodia seriata on apple. Res. Plant Dis. 24: 321-327. (In Korean) https://doi.org/10.5423/RPD.2018.24.4.321
  8. Korean Statistical Information Service. 2019. Area of cultivated field fruit trees. URL http://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1ET0014 [30 February 2019].
  9. Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35: 1547-1549. https://doi.org/10.1093/molbev/msy096
  10. Lee, D., Hassan, O., Kim, C. and Chang, T. 2018. First report of peach (Prunus persica) anthracnose caused by Colletotrichum fioriniae in Korea. Plant Dis. 102: 2650.
  11. Lee, H. W., Nguyen, T. T. T. and Lee, H. B. 2016. First report of postharvest fruit rot of Aronia melanocarpa caused by Fusarium tricinctum in Korea. Korean J. Mycol. 44: 68-71. https://doi.org/10.4489/KJM.2016.44.1.68
  12. O'Donnell, K., Kistler, H. C., Cigelnik, E. and Ploetz, R. C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc. Natl. Acad. Sci. U. S. A. 95: 2044-2049. https://doi.org/10.1073/pnas.95.5.2044
  13. O'Donnell, K. and Cigelnik, E. 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol. Phylogenet. Evol. 7: 103-116. https://doi.org/10.1006/mpev.1996.0376
  14. The Korean Society of Plant Pathology. 2009. List of Plant Disease in Korea. 5th ed. The Korean Society of Plant Pathology, Suwon, Korea. 859 pp. (In Korean)
  15. White, T. J., Bruns, T. D., Lee, S. B. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, New York, NY, USA.