한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제36권2호
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  • Pages.600-608
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  • 2019
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  • 1225-9098(pISSN)
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  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
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울금(Curcuma longa L.)의 생리활성 및 지질과산화 저해능에 미치는 영향

Effects of Turmeric (Curcuma longa L.) Bioactivity Compounds and Lipid Peroxidation Inhibitory Action

  • Oh, Da-Young (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • 투고 : 2019.05.01
  • 심사 : 2019.06.26
  • 발행 : 2019.06.30
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초록

울금(Curcuma longa L.)의 생리활성 및 지질과산화 저해능에 미치는 영향을 확인하고 기능성 식품 소재로 활용 가치를 검토하기 위하여 연구를 수행하였다. 총 카로티노이드(total carotenoid) 함량은 $1.581{\pm}0.005mg$ ${\beta}$-carotene equivalents (BCE)/g dry weight으로 관찰되었다. 70% 메탄올, chloroform:methanol (CM, 2:1, v/v), 에틸 아세테이트(ethyl acetate, EA) 3가지 용매를 사용한 추출 수율은 70% 메탄올(16.54%), CM (5.64%), EA (4.14%) 순으로 나타났다. 총 페놀 함량은 EA, CM 및 70% 메탄올에서 각각 106.287, 90.614 및 18.527 mg gallic acid equivalents (GAE)/g의 함량으로 나타났으며, 추출 용매 별 항산화능은 0.2, 0.4, 0.6, 0.8 mg/mL의 농도로 측정하였고, 양성대조구로 사용된 BHA (butylated hydroxyanisole) 및 trolox 보다는 낮은 항산화능을 보였다. Nitric oxide (NO) 라디칼 소거능은 70% 메탄올에서 28.65~48.43%, CM 18.86~55.10%, EA에서 15.68~56.25%로 관찰되었다. Nitrite ($NO_2$) 소거능은 70% 메탄올, CM 및 EA 순으로 나타나 EA 추출물에서 유의적인 차이를 보이며 강한 $NO_2$ 소거능을 나타내었다(p<0.05). ${\beta}$-carotene 탈색 저해능은 70% 메탄올, CM 및 EA에서 각각 1.64~23.79%, 6.99~41.16% 및 10.20~48.52%로 관찰되었다. 한편, 지질과산화 저해능은 70% 메탄올, CM 및 EA 추출물에서 높게 측정되었다.

The aim was to determine the physiological activity and antioxidant activity by lipid peroxidation inhibitory action of turmeric (Curcuma longa L.). Bioactive compound of total carotenoid $1.581{\pm}0.005mg$ ${\beta}$-carotene equivalents (BCE)/g dry weight. Total phenol content was the highest in the ethyl acetate (EA) extract, followed by chloroform:methanol (CM, 2:1, v/v) and 70% methanol extracts. Antioxidant effects (nitrogen oxide radical scavenging activity, nitrite scavenging activity, ${\beta}$-carotene bleaching assay, and lipid peroxidation inhibition action) of 70% methanol, CM, and EA extract of turmeric. Turmeric extracts yield were 70% methanol 16.54%, CM 5.64%, and EA 4.14%, respectively. Antioxidant activity of the samples exhibited a dose-dependent increase. However, in the current study, none of the samples evaluated showed activity as strong as the BHA (butylated hydroxyanisole) and trolox. Further, nitrite scavenging activity was the highest for the EA extract. As a result of this experiment, indicating their commercial value and potential applications in food and nutraceuticals.

키워드

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Fig. 1. Nitric oxide (NO) radical scavenging activity of various solvent extracts from turmeric (Curcuma longa L.).

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Fig. 2. Nitrite (NO2) scavenging activity of various solvent extracts from turmeric (Curcuma longa L.).

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Fig. 3. Antioxidant activity by β-carotene (BC) bleaching assay of various solvent extracts from turmeric (Curcuma longa L.).

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Fig. 4. Lipid peroxidation inhibition (LPI) action of various solvent extracts from turmeric (Curcuma longa L.).

Table 1. Contents of total carotenoid and total phenol in the bioactivity evaluation assays from turmeric (Curcuma longa L.)

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Table 2. IC50 values of NO radical scavenging activity, NO2 scavenging activity, antioxidant activity by BC bleaching assay and LPI action by turmeric (Curcuma longa L.)

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