Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Improving Effect to Connitive Ability of Cordyceps militaris Extract in PC12 and BV2 cells

PC12와 BV2 세포에서 동충하초 추출물의 인지능력 개선 효과

  • Choi, Soon-Hee (Department of beauty healthcare, Graduate School, Daejeon University) ;
  • Seung, O-Tak (Department of Oriental Health Care, Joongbu University) ;
  • Lee, Myung-Sun (Department of beauty healthcare, Graduate School, Daejeon University)
  • 최순희 (대전대학교 자연과학대학 뷰티건강관리학과 대학원) ;
  • 승오탁 (중부대학교 한방건강관리학과) ;
  • 이명선 (대전대학교 자연과학대학 뷰티건강관리학과 대학원)
  • Received : 2019.06.07
  • Accepted : 2019.06.18
  • Published : 2019.06.30
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Abstract

The aim of this study is to evaluate the efficacy of cordyceps militaris extracts for the improvement of cognitive dysfunction in PC12 and BV2 cells. Cordyceps militaris extracts was prepared by extracting with distilled water. Cell viability was assessed by MTT assay using PC12 cells and BV2 cells. Confirmed effects of L-glutamate induced cytotoxicity test, Acetylcoline (ACh) concentration, and Acetylcolinestase (AChE) activity in PC12 cells. Anti-inflammatory activities of cordyceps militaris extracts was measured through changes in the levels of nitric oxide (NO), and prostaglandin E2 ($PGE_2$) on lipopolysaccharide(LPS)-induced BV2 cell. In addition, we measured the expression of $NF-{\kappa}B$, p38, JNK, and caspase-3 in western blot analysis. Cordyceps militaris extracts showed no cytotoxicity at the concentrations of 1, 10, and $100{\mu}g/m{\ell}$ except for the concentration of $200{\mu}g/m{\ell}$. Cordyceps militaris extracts protected the cell and exhibited significant increases in the ACh concentration and a significant decrease in the AChE activity in L-glutamate induced PC12 cells. Moreover, cordyceps militaris extracts inhibited the productions NO, and PGE2 level and the protein expression of $NF-{\kappa}B$, p38, JNK, caspase-3 in LPS-induced BV2 cells. These results indicate that cordyceps militaris extracts possible prevented and improved cognitive dysfuction symptoms. Thus, cordyceps militaris extracts may be a novel natural material option for the improvement of cognitive dysfunction.

본 연구는 동충하초 추출물을 PC12 및 BV2 세포에서 인지능력 개선에 대한 효능을 평가하고자 하였다. 동충하초 추출물을 증류수로 추출하여 PC12 및 BV2 세포로 MTT 분석을 통해 세포 생존율을 확인하고 L-glutamate로 유도한 PC12 세포를 통해 세포 보호 효능과 아세틸콜린 함량 및 아세틸콜린에스테아제 활성을 평가하였다. 또한, LPS로 유도한 BV2 세포를 통해 nitric oxide (NO) 및 prostaglandin E2 (PGE2) 생성량 등의 항염증 효능을 측정하고 western blot 분석을 통해 $NK-{\kappa}B$, p38, JNK, caspase-3 등의 단백질 발현량을 확인하였다. 동충하초 추출물은 $200{\mu}g/m{\ell}$ 농도를 제외하고 1, 10, $100({\mu}g/m{\ell})$ 농도에서 세포 독성이 나타나지 않았다. L-glutamate로 유도한 PC12 세포에서 유의성있게 세포 보호 효능과 아세틸콜린 함량의 증가, 아세틸콜린에스테아제 활성 감소가 나타났다. 또한, 동충하초 추출물은 NO 및 PGE2 생성량과 $NK-{\kappa}B$, p38, JNK, caspase-3 등의 단백질 발현을 억제하였다. 이와 같은 결과는 동충하초 추출물이 인지능력에 대한 예방 및 개선 효능이 있음을 나타낸다. 따라서 동충하초 추출물은 인지능력 개선을 위한 새로운 천연 소재로 활용될 수 있다.

Keywords

HGOHBI_2019_v36n2_468_f0001.png 이미지

Fig. 1. Effects of cell viability on CM extracts in PC12 (A) and BV2 (B) cells.

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Fig. 2. Effects of L-glutamate-induced cell viability on CM extracts in PC12 cells.

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Fig. 3. Effects of L-glutamate-induced acetylcholine concentration(A), and Acetylcholinesterase activity(B) on CM extracts in PC12 cells.

HGOHBI_2019_v36n2_468_f0004.png 이미지

Fig. 4. Effects of LPS-induced nitric oxide (A), and prostaglandin E2 (B) level on CM extracts in BV2 cells.

HGOHBI_2019_v36n2_468_f0005.png 이미지

Fig. 5. Effects of LPS-induced NK-ĸB, p38, JNK, and caspase-3 protein expression on CM extracts in BV2 cells.

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