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http://dx.doi.org/10.12925/jkocs.2019.36.2.468

Improving Effect to Connitive Ability of Cordyceps militaris Extract in PC12 and BV2 cells  

Choi, Soon-Hee (Department of beauty healthcare, Graduate School, Daejeon University)
Seung, O-Tak (Department of Oriental Health Care, Joongbu University)
Lee, Myung-Sun (Department of beauty healthcare, Graduate School, Daejeon University)
Publication Information
Journal of the Korean Applied Science and Technology / v.36, no.2, 2019 , pp. 468-478 More about this Journal
Abstract
The aim of this study is to evaluate the efficacy of cordyceps militaris extracts for the improvement of cognitive dysfunction in PC12 and BV2 cells. Cordyceps militaris extracts was prepared by extracting with distilled water. Cell viability was assessed by MTT assay using PC12 cells and BV2 cells. Confirmed effects of L-glutamate induced cytotoxicity test, Acetylcoline (ACh) concentration, and Acetylcolinestase (AChE) activity in PC12 cells. Anti-inflammatory activities of cordyceps militaris extracts was measured through changes in the levels of nitric oxide (NO), and prostaglandin E2 ($PGE_2$) on lipopolysaccharide(LPS)-induced BV2 cell. In addition, we measured the expression of $NF-{\kappa}B$, p38, JNK, and caspase-3 in western blot analysis. Cordyceps militaris extracts showed no cytotoxicity at the concentrations of 1, 10, and $100{\mu}g/m{\ell}$ except for the concentration of $200{\mu}g/m{\ell}$. Cordyceps militaris extracts protected the cell and exhibited significant increases in the ACh concentration and a significant decrease in the AChE activity in L-glutamate induced PC12 cells. Moreover, cordyceps militaris extracts inhibited the productions NO, and PGE2 level and the protein expression of $NF-{\kappa}B$, p38, JNK, caspase-3 in LPS-induced BV2 cells. These results indicate that cordyceps militaris extracts possible prevented and improved cognitive dysfuction symptoms. Thus, cordyceps militaris extracts may be a novel natural material option for the improvement of cognitive dysfunction.
Keywords
cordyceps militaris; neurons cells; microglia cells; improvement of cognitive dysfunction; herbal raw material;
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