The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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The Anti-inflammatory Effects of Heat-treated Broccoli Extract

열처리된 브로콜리 추출물의 항염증 효과

  • 김현경 (서원대학교 식품공학과)
  • Received : 2019.02.16
  • Accepted : 2019.04.16
  • Published : 2019.05.31
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Abstract

This study was carried out to investigate the heat-treated broccoli extraction which have a beneficial effect on the human body and which can be used safely for a long period of time without adverse side effects and also have excellent effects of protecting liver and improving liver function. The heat-treated broccoli extract does not show cytotoxicity, and thus can be used safely. In an experiment performed on an animal model with liver injury induced by a drug (APAP), it could be seen that the heat-treated cabbage extract exhibited the effects of protecting liver and improving liver function by effectively reducing AST and ALT which are liver injury markers, indicating that the heat-treated brocoli extract is effective as a pharmaceutical extraction for preventing or treating liver disease. In particular, the heat-treated broccoli extract was effective in treating inflammation of the liver by reducing the expression of the inflammatory mediators iNOS and COX-2 and the proinflammatory cytokine $IL-1{\beta}$, which are involved in acute inflammatory reactions accompanying liver injury.

본 연구는 인체에 유익한 효능을 지니고 부작용없이 장기간 안전하게 사용할 수 있으며 간보호 및 간기능 개선에 탁월한 효능을 갖는 가열 브로콜리 추출물을 연구하기 위해 수행되었다. 열처리된 브로콜리 추출물은 세포 독성을 나타내지 않으므로 안전하게 사용할 수 있었다. 약제에 의한 간손상 동물모델 (APAP)에서 수행 한 실험에서, 열처리된 양배추 추출물은 간손상 마커인 AST 및 ALT를 효과적으로 감소시킴으로써 간보호 및 간기능 개선 효과를 나타냄을 알 수 있었고, 이는 열처리된 브로콜리 추출물이 간질환을 예방하거나 치료하기 위한 약학적 추출물로서 효과적이라는 것을 시사한다. 특히, 열처리된 브로콜리 추출물은 간손상을 동반한 급성염증 반응에 관여하는 염증 매개체 iNOS 및 COX-2 및 염증 유발성 사이토카인 $IL-1{\beta}$의 발현을 감소시킴으로써 간염 염증을 치료하는데 효과적이라고 할 수 있다.

Keywords

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그림 1. 브로콜리 추출물의 세포독성 (cell Viability) 평가 Figure 1. Cell viability assay in RAW 264.7 cells treated with broccoli extracts.

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그림 2. 마우스의 혈청에서 (A) Superoxide Dismutase (SOD), (B) 글루타티온 퍼 옥시 다제 (GPx) 및 (C) 카탈라아제 활성 (CAT) 측정 Figure 2. (A) Superoxide dismutase (SOD), (B) glutathione peroxidase (GPx) and (C) catalase activity (CAT) in the serum of mice. € indicates p < 0.05 as compared to the control group, *** indicates p < 0.001 compared to acetaminophen, and # indicates p < 0.01 as compared to the positive control, NAC.

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그림 3. 마우스의 혈청에서 8-Oxo-2'-dexoxyguanosine (8-OHdG) 수준 Figure 3. 8-Oxo-2’-dexoxyguanosine (8-OHdG) levels in the serum of mice. #indicates p < 0.05 as compared to the control group, *** indicates p < 0.001 compared to acetaminophen, and # indicates p < 0.01 as compared to the positive control, NAC.

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그림 4. 혈청에서 (A) Alanine aminotransferase (ALT) and (B) aspartate transaminase (AST) 레벨 측정 Figure 4. Figure. 1. (A) Alanine aminotransferase (ALT) and (B) aspartate transaminase (AST) levels in the serum. $ indicates p < 0.05 as compared to the control group, *** indicates p < 0.001 compared to acetaminophen, and # indicates p < 0.01 as compared to the positive control, NAC.

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그림 5. 혈청에서 (A) Alanine aminotransferase (ALT) and (B) aspartate transaminase (AST) 레벨 측정 Figure 5. Figure. 1. (A) Alanine aminotransferase (ALT) and (B) aspartate transaminase (AST) levels in the serum. $ indicates p < 0.05 as compared to the control group, *** indicates p < 0.001 compared to acetaminophen, and # indicates p < 0.01 as compared to the positive control, NAC.

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그림 6. 간 조직의 Hematoxylin & Eosin 염색 Figure 6.. Hematoxylin & Eosin (H&E) staining of sections of the liver of mice in group (A) control, (B) acetaminophen, (C) NAC, (D) cabbage. Images were taken at a magnification of 400x under a microscope.

표 1. RT_PCR에 의한 사이토카인 발현 측정 Table 1. Measurement of cytokine expression by RT-PCR

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