Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effects of Ethanol Extract from Aster Yomena on Acute Pancreatitis

쑥부쟁이 에탄올 추출물의 급성췌장염 보호 효과

  • 서상완 (호남대학교 바이오융합학과)
  • Received : 2019.02.25
  • Accepted : 2019.04.24
  • Published : 2019.04.25
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Abstract

Aster Yomena (AY) has been used in traditional medicine to treat diseases such as obesity, hyperlipidemia, atherosclerosis, diabetes and osteoarthritis. However, protective effect of AY on acute pancreatitis (AP) has not been reported. The present study examined the anti-inflammatory effects of an ethanol extract of AY on cerulein-induced AP. AP was induced in mice by intraperitoneally injecting cerulein ($50{\mu}g/kg$) hourly for 6 times. 70% ethanol extract of AY (0.1, 0.2, and 0.5 g/kg) was orally administered for 1 week before acute pancreatitis induction. The mouse was killed at 6 hours after the final cerulein injection. The pancreas and lung were rapidly removed for histological examination and myeloperoxidase (MPO) assay. Blood samples were taken to determine serum amylase and lipase activity. In addition real-time reverse transcription-polymerase chain reaction (RT-PCR) was also performed to investigate mRNA expression of proinflammatory cytokines such as $TNF-{\alpha}$. $IL-1{\beta}$, and IL-6. Administration of AY significantly ameliorated pancreatic weight to body weight ratio, histological damages and MPO activity during AP. In addition, AY inhibited the serum amylase and lipase activity during AP. Also, mRNA expression of $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6 were inhibited by AY against AP. Our results revealed that pre-treatment of AY reduces the severity of cerulein-induced AP. Therefore, AY may have a protective effect drug against AP.

Keywords

References

  1. Zhou MT, Chen CS, Chen BC, Zhang QY, Andersson R. Acute lung injury and ARDS in acute pancreatitis: mechanisms and potential intervention. World J Gastroenterol. 2010;16(17):2094-9. https://doi.org/10.3748/wjg.v16.i17.2094
  2. Andersson R, Andersson B, Haraldsen P, Drewsen G, Eckerwall G. Incidence, management and recurrence rate of acute pancreatitis. Scand J Gastroenterol. 2004;39(9):891-4. https://doi.org/10.1080/00365520410007061
  3. Kingsnorth A, O'Reilly D. Acute pancreatitis. Br Med J. 2006;332:1072-6. https://doi.org/10.1136/bmj.332.7549.1072
  4. Steinberg W, Tenner S. Acute pancreatitis. N Engl J Med. 1994;330:1198-210. https://doi.org/10.1056/NEJM199404283301706
  5. Pandol SJ, Saluja AK, Imrie CW, Banks PA. Acute pancreatitis: bench to the bedside. Gastroenterology. 2007;132(3):1127-51. https://doi.org/10.1053/j.gastro.2007.01.055
  6. Beger HG, Rau B, Isenmann R. Natural history of necrotizing pancreatitis. Pancreatology. 2003;3(2):93-101. https://doi.org/10.1159/000070076
  7. Yoon YK, Ji JH, Mun BS. Hypertriglyceridemiainduced pancreatitis. Korean J Gastroenterol. 2008;51:309-13.
  8. Wang GJ, Gao CF, Wei D, Wang C, Ding SQ. Acute pancreatitis: Etiology and common pathogenesis. World J Gastroenterol. 2009;15:1427-30. https://doi.org/10.3748/wjg.15.1427
  9. Baron TH, Morgan DE. Acute necrotizing pancreatitis. N Engl J Med. 1999;340:1412-7. https://doi.org/10.1056/NEJM199905063401807
  10. Ho HS, Frey CF. Gastrointestinal and pancreatic complications associated with severe pancreatitis. Arch Surg. 1995;130:817-22. https://doi.org/10.1001/archsurg.1995.01430080019002
  11. Kapoor LD. CRC handbook of ayurvedic medicinal plants. Boca Raton: CRC Press; 1990.
  12. Yoon HR, Yook CS. Studies on the constituents of Angelica gigias. Bull KH pharma Sci. 1995;23:55-71.
  13. Jung BM, Lim SS, Park YJ, Bae SJ. Inhibitory effects on cell survival and quinone reductase induced activity of Aster yomena fractions onhuman cancer cells. J Korean Soc Food Sci Nutri. 2005;34:8-12. https://doi.org/10.3746/jkfn.2005.34.1.008
  14. Chon SU, Kim DI, Choi YS. Assessments on insecticidal and fungicidal activities by aerial part extracts from several compositae plants. Kor J Weed Sci. 2003;23:81-91.
  15. Kim MJ, Kim JH, Lee SH, Cho EJ, Kim HY. Determination of radical scavenging activity of aster yomena. Journal of the Korea Academia-Industrial Cooperation Society. 2018;19(9):402-7. https://doi.org/10.5762/KAIS.2018.19.9.402
  16. Bae JS, Kim TH. Acetylcholinesterase inhibitory and antioxidant properties of aster yomena extract. Kor J Herbology. 2009;24(4):121-6.
  17. Lee HJ, Kim HS, Seo SW. Anti-obesity effect of aster yomena ethanol extract in high fat diet-induced obese mice. J Physiol & Pathol Korean Med 2017;31(6):348-55. https://doi.org/10.15188/kjopp.2017.12.31.6.348
  18. Nissen SE, Wolski K, Topol EJ. Effect of muraglitazar on death and major adverse cardiovascular events in patients with type 2 diabetes mellitus. JAMA. 2005;294:2581-6. https://doi.org/10.1001/jama.294.20.joc50147
  19. Bhatia M, Wong FL, Cao Y, et al. Pathophysiology of acute pancreatitis. Pancreatology. 2005;5(2-3):132-44. https://doi.org/10.1159/000085265
  20. Regner S, Manjer J, Appelros S, Hjalmarsson C, Sadic J, Borgstrom A. Protease activation, pancreatic leakage, and inflammation in acute pancreatitis: differences between mild and severe cases and changes over the first three days. Pancreatology. 2008;8(6):600-7. https://doi.org/10.1159/000161011
  21. Buchler MW, Gloor B, Muller CA, Friess H, Seiler CA, Uhl W. Acute necrotizing pancreatitis: treatment strategy according to the status of infection. Ann Surg. 2000;232(5):619-26. https://doi.org/10.1097/00000658-200011000-00001
  22. Rau BM, Krüger CM, Schilling MK. Anti-cytokine strategies in acute pancreatitis: pathophysiological insights and clinical implications. Rocz Akad Med Bialymst. 2005;50:106-15.
  23. Kim SC, Yang HR. Clinical efficacy of gabexate mesilate for acute pancreatitis in children. Eur J Pediatr. 2013;172(11):1483-90. https://doi.org/10.1007/s00431-013-2068-6
  24. Jenkins DJ, Kendall CW, Augustin LS, et al. Glycemic index: Overview of implications in health and disease. Am J clin Nutr. 2002;76(1):266S-73S. https://doi.org/10.1093/ajcn/76.1.266S
  25. Kang HJ, Jeong JS, Park NJ, et al. An ethanol extract of Aster yomena (Kitam.) Honda inhibits lipopolysaccharide-induced inflammatory responses in murine RAW 264.7 macrophages. Biosci Trends. 2017;11(1):85-94. https://doi.org/10.5582/bst.2016.01217
  26. Hwang KA, Hwang YJ, Song J. Aster yomena extract ameliorates pro-inflammatory immune response by suppressing $NF-{\kappa}B$ activation in RAW 264.7 cells. J Chin Med Assoc. 2018;81(2):102-10. https://doi.org/10.1016/j.jcma.2017.06.017
  27. Kim TH, Bae Gs, Oh HJ, et al. 2',4',6'-Tris (methoxymethoxy) chalcone (TMMC) attenuates the severity of cerulein-induced acute pancreatitis and associated lung injury. Am J Physiol Gastrointest Liver Physiol. 2001;301(4):694-706.
  28. Bae Gs, Kim MS, Park KC, et al. Effect of biologically active fraction of Nardostachys jatamansi on cerulein-induced acute pancreatitis. World J Gastroenterol. 2012;18(25):3223-34. https://doi.org/10.3748/wjg.v18.i25.3223
  29. Cope O, Culver PJ, Mixter CG Jr, Nardi GL. Pancreatitis, a diagnostic clue to hyperparathyroidism. Ann Surg. 1957;145(6):857-63. https://doi.org/10.1097/00000658-195706000-00007
  30. Lee HS. Experimental animal models of pancreatic disease. Kor J Gastroenterol seminar. 2002:144-51.
  31. Granger J, Remick D. Acute pancreatitis: models, markers, nad mediators. Shock. 2005;24(1):45-51. https://doi.org/10.1097/01.shk.0000191413.94461.b0
  32. Panteghini M, Pagani F, bonora R. Clinical and analytical evaluation of a continuous enzymatic methods for measuring pancreatic lipase activity. Clin chem. 1993;39(2):304-8. https://doi.org/10.1093/clinchem/39.2.304
  33. Jung WS, Chae YS, Kim DY, et al. Gardenia jasminoides protects against cerulein-induced acute pancreatitis. World J Gastroenterol. 2008;14(40):6188-94. https://doi.org/10.3748/wjg.14.6188
  34. Heath DI, Cruickshank A, Gudgeon AM, Jehanli A, Shenkin A, Imrie CW. The relationship between pancreatic enzyme release and activation and the acute-phase protein response in patients with acute pancreatitis. Pancreas. 1995;10(4):347-53. https://doi.org/10.1097/00006676-199505000-00005
  35. Jaworek J, Szklarczyk J, Kot M, et al. Chemerin alleviates acute pancreatitis in the rat thorough modulation of $NF-{\kappa}B$ signal. Pancreatology. 2019;19(3):401-8. https://doi.org/10.1016/j.pan.2019.02.005
  36. Colgan SP, Ehrentraut SF, Glover LE, Kominsky DJ, Campbell EL. Contributions of neutrophils to resolution of mucosal inflammation. Immunol Res. 2013;55(1-3):75-82. https://doi.org/10.1007/s12026-012-8350-2
  37. Interiano B, Stuard ID, Hyde RW. Acute respiratory distress syndrome in pancreatitis. Ann Int Med. 1972;77:923-6. https://doi.org/10.7326/0003-4819-77-6-923
  38. Guice KS, Oldham KT, Johnson KJ, Kunkel RG, Morganroth ML, Ward PA. Pancreatitits-induced acute lung injury, an ARDS model. Ann Surg. 1988;208(1):71-7. https://doi.org/10.1097/00000658-198807000-00010