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http://dx.doi.org/10.15188/kjopp.2019.04.33.2.109

Protective Effects of Ethanol Extract from Aster Yomena on Acute Pancreatitis  

Seo, Sang Wan (Department of Bio-Technology, Honam University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.33, no.2, 2019 , pp. 109-115 More about this Journal
Abstract
Aster Yomena (AY) has been used in traditional medicine to treat diseases such as obesity, hyperlipidemia, atherosclerosis, diabetes and osteoarthritis. However, protective effect of AY on acute pancreatitis (AP) has not been reported. The present study examined the anti-inflammatory effects of an ethanol extract of AY on cerulein-induced AP. AP was induced in mice by intraperitoneally injecting cerulein ($50{\mu}g/kg$) hourly for 6 times. 70% ethanol extract of AY (0.1, 0.2, and 0.5 g/kg) was orally administered for 1 week before acute pancreatitis induction. The mouse was killed at 6 hours after the final cerulein injection. The pancreas and lung were rapidly removed for histological examination and myeloperoxidase (MPO) assay. Blood samples were taken to determine serum amylase and lipase activity. In addition real-time reverse transcription-polymerase chain reaction (RT-PCR) was also performed to investigate mRNA expression of proinflammatory cytokines such as $TNF-{\alpha}$. $IL-1{\beta}$, and IL-6. Administration of AY significantly ameliorated pancreatic weight to body weight ratio, histological damages and MPO activity during AP. In addition, AY inhibited the serum amylase and lipase activity during AP. Also, mRNA expression of $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6 were inhibited by AY against AP. Our results revealed that pre-treatment of AY reduces the severity of cerulein-induced AP. Therefore, AY may have a protective effect drug against AP.
Keywords
Acute pancreatitis; Amylase; Aster Yomena; Cytokines; Lipase;
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1 Jenkins DJ, Kendall CW, Augustin LS, et al. Glycemic index: Overview of implications in health and disease. Am J clin Nutr. 2002;76(1):266S-73S.   DOI
2 Kang HJ, Jeong JS, Park NJ, et al. An ethanol extract of Aster yomena (Kitam.) Honda inhibits lipopolysaccharide-induced inflammatory responses in murine RAW 264.7 macrophages. Biosci Trends. 2017;11(1):85-94.   DOI
3 Hwang KA, Hwang YJ, Song J. Aster yomena extract ameliorates pro-inflammatory immune response by suppressing $NF-{\kappa}B$ activation in RAW 264.7 cells. J Chin Med Assoc. 2018;81(2):102-10.   DOI
4 Kim TH, Bae Gs, Oh HJ, et al. 2',4',6'-Tris (methoxymethoxy) chalcone (TMMC) attenuates the severity of cerulein-induced acute pancreatitis and associated lung injury. Am J Physiol Gastrointest Liver Physiol. 2001;301(4):694-706.
5 Bae Gs, Kim MS, Park KC, et al. Effect of biologically active fraction of Nardostachys jatamansi on cerulein-induced acute pancreatitis. World J Gastroenterol. 2012;18(25):3223-34.   DOI
6 Cope O, Culver PJ, Mixter CG Jr, Nardi GL. Pancreatitis, a diagnostic clue to hyperparathyroidism. Ann Surg. 1957;145(6):857-63.   DOI
7 Lee HS. Experimental animal models of pancreatic disease. Kor J Gastroenterol seminar. 2002:144-51.
8 Granger J, Remick D. Acute pancreatitis: models, markers, nad mediators. Shock. 2005;24(1):45-51.   DOI
9 Panteghini M, Pagani F, bonora R. Clinical and analytical evaluation of a continuous enzymatic methods for measuring pancreatic lipase activity. Clin chem. 1993;39(2):304-8.   DOI
10 Jung WS, Chae YS, Kim DY, et al. Gardenia jasminoides protects against cerulein-induced acute pancreatitis. World J Gastroenterol. 2008;14(40):6188-94.   DOI
11 Heath DI, Cruickshank A, Gudgeon AM, Jehanli A, Shenkin A, Imrie CW. The relationship between pancreatic enzyme release and activation and the acute-phase protein response in patients with acute pancreatitis. Pancreas. 1995;10(4):347-53.   DOI
12 Jaworek J, Szklarczyk J, Kot M, et al. Chemerin alleviates acute pancreatitis in the rat thorough modulation of $NF-{\kappa}B$ signal. Pancreatology. 2019;19(3):401-8.   DOI
13 Colgan SP, Ehrentraut SF, Glover LE, Kominsky DJ, Campbell EL. Contributions of neutrophils to resolution of mucosal inflammation. Immunol Res. 2013;55(1-3):75-82.   DOI
14 Interiano B, Stuard ID, Hyde RW. Acute respiratory distress syndrome in pancreatitis. Ann Int Med. 1972;77:923-6.   DOI
15 Guice KS, Oldham KT, Johnson KJ, Kunkel RG, Morganroth ML, Ward PA. Pancreatitits-induced acute lung injury, an ARDS model. Ann Surg. 1988;208(1):71-7.   DOI
16 Lee HJ, Kim HS, Seo SW. Anti-obesity effect of aster yomena ethanol extract in high fat diet-induced obese mice. J Physiol & Pathol Korean Med 2017;31(6):348-55.   DOI
17 Chon SU, Kim DI, Choi YS. Assessments on insecticidal and fungicidal activities by aerial part extracts from several compositae plants. Kor J Weed Sci. 2003;23:81-91.
18 Kim MJ, Kim JH, Lee SH, Cho EJ, Kim HY. Determination of radical scavenging activity of aster yomena. Journal of the Korea Academia-Industrial Cooperation Society. 2018;19(9):402-7.   DOI
19 Bae JS, Kim TH. Acetylcholinesterase inhibitory and antioxidant properties of aster yomena extract. Kor J Herbology. 2009;24(4):121-6.
20 Nissen SE, Wolski K, Topol EJ. Effect of muraglitazar on death and major adverse cardiovascular events in patients with type 2 diabetes mellitus. JAMA. 2005;294:2581-6.   DOI
21 Bhatia M, Wong FL, Cao Y, et al. Pathophysiology of acute pancreatitis. Pancreatology. 2005;5(2-3):132-44.   DOI
22 Regner S, Manjer J, Appelros S, Hjalmarsson C, Sadic J, Borgstrom A. Protease activation, pancreatic leakage, and inflammation in acute pancreatitis: differences between mild and severe cases and changes over the first three days. Pancreatology. 2008;8(6):600-7.   DOI
23 Buchler MW, Gloor B, Muller CA, Friess H, Seiler CA, Uhl W. Acute necrotizing pancreatitis: treatment strategy according to the status of infection. Ann Surg. 2000;232(5):619-26.   DOI
24 Rau BM, Krüger CM, Schilling MK. Anti-cytokine strategies in acute pancreatitis: pathophysiological insights and clinical implications. Rocz Akad Med Bialymst. 2005;50:106-15.
25 Pandol SJ, Saluja AK, Imrie CW, Banks PA. Acute pancreatitis: bench to the bedside. Gastroenterology. 2007;132(3):1127-51.   DOI
26 Kim SC, Yang HR. Clinical efficacy of gabexate mesilate for acute pancreatitis in children. Eur J Pediatr. 2013;172(11):1483-90.   DOI
27 Zhou MT, Chen CS, Chen BC, Zhang QY, Andersson R. Acute lung injury and ARDS in acute pancreatitis: mechanisms and potential intervention. World J Gastroenterol. 2010;16(17):2094-9.   DOI
28 Andersson R, Andersson B, Haraldsen P, Drewsen G, Eckerwall G. Incidence, management and recurrence rate of acute pancreatitis. Scand J Gastroenterol. 2004;39(9):891-4.   DOI
29 Kingsnorth A, O'Reilly D. Acute pancreatitis. Br Med J. 2006;332:1072-6.   DOI
30 Steinberg W, Tenner S. Acute pancreatitis. N Engl J Med. 1994;330:1198-210.   DOI
31 Beger HG, Rau B, Isenmann R. Natural history of necrotizing pancreatitis. Pancreatology. 2003;3(2):93-101.   DOI
32 Yoon YK, Ji JH, Mun BS. Hypertriglyceridemiainduced pancreatitis. Korean J Gastroenterol. 2008;51:309-13.
33 Wang GJ, Gao CF, Wei D, Wang C, Ding SQ. Acute pancreatitis: Etiology and common pathogenesis. World J Gastroenterol. 2009;15:1427-30.   DOI
34 Baron TH, Morgan DE. Acute necrotizing pancreatitis. N Engl J Med. 1999;340:1412-7.   DOI
35 Ho HS, Frey CF. Gastrointestinal and pancreatic complications associated with severe pancreatitis. Arch Surg. 1995;130:817-22.   DOI
36 Kapoor LD. CRC handbook of ayurvedic medicinal plants. Boca Raton: CRC Press; 1990.
37 Yoon HR, Yook CS. Studies on the constituents of Angelica gigias. Bull KH pharma Sci. 1995;23:55-71.
38 Jung BM, Lim SS, Park YJ, Bae SJ. Inhibitory effects on cell survival and quinone reductase induced activity of Aster yomena fractions onhuman cancer cells. J Korean Soc Food Sci Nutri. 2005;34:8-12.   DOI