Fig. 2. Effect of TKM (Trichosanthes kirilowii Maxim. root) ethanol extract on Oil Red O staining and triglyceride contents in differentiated 3T3-L1 adipocytes. A, captured with a microscope; B, quantified by the lipid accumulation using spectrophotometer. Results were expressed as the mean±SE of three independent experiments. Student’s t-test was performed using GraphPad Prism 5 program. *P<0.05, control vs. TKM. Fenofibrate was a positive control.
Fig. 1. Effect of the Trichosanthes kirilowii Maxim root (TKM) on viability of 3T3-L1 cells. MTT assay. Results were expressed as the mean±SD of three independent experiments. Student’s t-test was performed using GraphPad Prism 5 program. NS, none significance, *P<0.05, control vs. TKM.
Fig. 3. Effect of TKM extract on mRNA (black) and protein (white) expressions in the 3T3-L1 cells. GAPDH and β-actin was used as a loading control in RT-PCR and western blot. Vehicle control (-), Resveratrol as positive control (+) and TKM treated cells. TKM; 100 μg/ml Trichosanthes kirilowii Maxim ethanol extracts.
Table 1. Oligonucleotides sequence used in PCR
References
- OECD. (2018). OECD Health Statistics. http://www.oecd.org
- The Ministry of Health and Welfare(MOHW). Korea Centers for Disease Control & Prevention Korea National Health and Nutrition Examination Survey (2017), https://knhanes.cdc.go.kr/knhanes/sub05/sub05_01_view.do.
- Guidelines for Obesity therapy (2018). Korean Society for the Study of Obesity (KSSO). http://www.kosso.or.kr/file/file180614.pdf
- Y. Huang, P. He, K. P. Bader, A. Radunz & G. H. Schmid. (2000). Seeds of Trichosanthes kirilowii, an energy-rich diet. Zeitschrift fur Naturforschung. C J biosci. 55(3), 189-194. DOI : 10.1515/znc-2000-3-409
- T. Akihisa, W. C. M. C. Kokke, J. A. Krause, T. Tamura, D. S. Eggleston, S. I. Katayama, Y. Kimura & T. Tamura. (1992). 5-Dehydrokarounidiol [ D - C - Friedo - Oleana - 5, 7, 9(11)-Triene-3-Alpha,29-Diol], a novel triterpene from Trichosanthes kirilowii Maxim. Chem Pharm Bull. 40(12), 3280-3283. DOI : 10.1248/cpb.40.3280
- T. Akihisa, W. C. M. C. Kokke, T. Tamura & T. Nambara. (1992). 7-Oxodihydrokarounidiol [7-Oxo-Dc- Friedo-Olean8-Ene-3-Alpha,29-Diol], a novel triterpene from Trichosanthes kirilowii. Chem Pharm Bull. 40(5), 1199-1202. DOI : 10.1248/cpb.40.1199
- X. M. Fan, G. Chen, Y. Sha, X. Lu, M. Shen, H. M. Ma, & Y. H. Pei. (2012). Chemical constituents from the fruits of Trichosanthes kirilowii. J Asian Nat Prod Res. 14(6), 528-532. DOI : 10.1080/10286020.2012.672410
- J. Kitajima & Y. Tanaka. (1989). Studies on the constituents of trichosanthes root. I. Constituents of roots of Trichosanthes kirilowii Maxim. var. japonicum Kitam. Yakugaku zasshi. 109(4), 250-255. DOI : None https://doi.org/10.1248/yakushi1947.109.4_250
- Y. Ozaki, L. Xing & M. Satake. (1996). Antiinflammatory effect of Trichosanthes kirilowii Maxim. and its effective parts. Biol Pharm Bull. 19(8), 1046-1048. DOI : 10.1248/bpb.19.1046
- T. C. Otto, M. D. Lane (2005). Adipose development: from stem cell to adipocyte. Crit Rev Biochem Mol Biol. 40(4), 229-42. DOI : 10.1080/10409230591008189
- S. Ambati, J. Y. Yang, S. Rayalam, H. J. Park, M. A. Della-Fera & C. A. Baile. (2009). Ajoene exerts potent effects in 3T3-L1 adipocytes by inhibiting adipogenesis and inducing apoptosis. Phytother Res. 23(4), 513-518. DOI : 10.1002/ptr.2663
- J. A. Lee, Y. J. Park, W. S. Jeong & S. S. Hong. (2017). Anti-obesity effect of Amomum taso-ko ethanol extract in 3T3-L1 adipocytes. J Appl Bio Chem. 60(1), 23-28. DOI : https://doi.org/10.3839/jabc.2017.005
- B. B. Zhang, G. Zhou & C. Li. (2009). AMPK: an emerging drug target for diabetes and the metabolic syndrome. Cell Metab. 9(5), 407-416. DOI : 10.1016/j.cmet.2009.03.012
- L. Orci, W. S. Cook, M. Ravazzola, M. Y. Wang, B. H. Park, R. Montesano & R. H. Unger (2004). Rapid transformation of white adipocytes into fat-oxidizing machines. Proc. Natl. Acad. Sci. USA 101(7), 2058-2063. DOI: 10.1073/pnas.0308258100
-
M. M. Gonzalez-Barroso, A. Anedda, E. Gallardo-Vara, M. Redondo-Horcajo, L. Rodriguez-Sanchez & E. Rial. (2012). Fatty acids revert the inhibition of respiration caused by the antidiabetic drug metformin to facilitate their mitochondrial
${\beta}$ -oxidation. Biochim Biophys Acta. 1817(10), 1768-1775. DOI : 10.1016/j.bbabio.2012.02.019 - D. G. Hardie. (2004). The AMP-activated protein kinase pathway-new players upstream and downstream. J Cell Sci 117(23), 5479-5487. DOI : 10.1242/jcs.01540