Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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A convergence study of cytotoxicity evaluation of adult dentifrices

성인용 치약의 세포독성 평가의 융합적 연구

  • Choi, Yu-Ri (Department of Dental hygiene, Hallym polytechnic University) ;
  • Shim, Youn-Soo (Department of Dental hygiene, Sunmoon University) ;
  • Jang, Sun-Ok (Department of Dental hygiene, Hallym polytechnic University)
  • 최유리 (한림성심대학교 치위생과) ;
  • 심연수 (선문대학교 치위생학과) ;
  • 장선옥 (한림성심대학교 치위생과)
  • Received : 2018.12.31
  • Accepted : 2019.03.20
  • Published : 2019.03.28
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Abstract

This study was conducted to effect the cell activity and cytotoxicity of dentifrice. For the study, 6 kinds of general dentifrice, 3 kind of whitening dentifrice, 2 kinds of natural dentifrice and SLS(sodium lauryl sulfate) of positive control group. Immortalized human gingiva fibroblast cell was used for the study, WST test for cell activity and Agar diffusion test for cytotoxicity. Agar diffusion test showed high cytotoxicity in general dentifrice test group and whitening dentifrice test group, but low cytotoxicity in natural dentifrice test group. As a result of cell nucleus staining, cell shape and nuclear activity showed that the highest activity in natural dentifrice group, followed by whitening dentifrice group and general dentifrice group. As a result of this study, the cytotoxicity of different ingredient and according to the use to dentifrice. As a result of this study, we confirm cytotoxicity of kind and components according to the purpose of using dentifrice. Therefore, it is necessary to indicate the detailed ingredients of dentifrice for the smart choice of consumers.

본 논문은 치약의 성분이 구강 내 세포에 영향을 미치는 정도를 확인하기 위하여 세포활성도, 세포독성을 평가하고자 하였다. 일반치약 6종, 미백치약 3종, 천연치약 2종, 양성대조군으로 SLS(sodium lauryl sulfate)를 사용하였다. Immortalized human gingiva fibroblast cell 을 사용하여, 세포활성도 평가를 위하여 WST test, 세포 독성평가를 위해 Agar diffusion test를 시행하였다. 일반치약그룹, 미백치약그룹, 천연치약그룹 순으로 세포 생존률이 높게 나타났으며, Agar diffusion test는 일반치약그룹과 미백치약그룹은 높은 세포독성을 나타낸 반면 천연치약그룹은 낮은 독성을 나타냈다. 세포핵염색 결과 세포모양과 핵활성도 또한 천연치약이 가장 높은 활성도를 나타냈고, 미백치약, 일반치약 순으로 나타났다. 본 연구 결과, 치약의 사용목적에 따라 나누어지는 종류와 성분별 세포독성을 확인할 수 있었다. 소비자의 올바른 선택을 위해 치약의 자세한 성분표기가 필요할 것으로 사료된다.

Keywords

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Fig. 1. Cell viability result of adults dentifrice * different alphabet was statistically significant difference

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Fig. 2. Result of cell morphology and nucleus activity (a) G-1 group, (b) G-2 group, (c) G-3 group, (d) G-4 group, (e) G-5 group, (f) G-6 group, (g) W-1 group, (h) W-2 group, (i) W-3 group, (j) N-1 group, (k) N-2 group (l) PC

Table 1. Appliance of dentifrice materials of this study

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Table 2. Decolorization index

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Table 3. Cell activity result of dentifrice

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Table 4. Agar diffusion assay zone grade result

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