디지털융복합연구 (Journal of Digital Convergence)

  • 제17권3호
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  • Pages.305-312
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  • 2019
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  • 2713-6434(pISSN)
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  • 2713-6442(eISSN)
한국디지털정책학회 (The Society of Digital Policy and Management)
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성인용 치약의 세포독성 평가의 융합적 연구

A convergence study of cytotoxicity evaluation of adult dentifrices

  • 최유리 (한림성심대학교 치위생과) ;
  • 심연수 (선문대학교 치위생학과) ;
  • 장선옥 (한림성심대학교 치위생과)
  • Choi, Yu-Ri (Department of Dental hygiene, Hallym polytechnic University) ;
  • Shim, Youn-Soo (Department of Dental hygiene, Sunmoon University) ;
  • Jang, Sun-Ok (Department of Dental hygiene, Hallym polytechnic University)
  • 투고 : 2018.12.31
  • 심사 : 2019.03.20
  • 발행 : 2019.03.28
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초록

본 논문은 치약의 성분이 구강 내 세포에 영향을 미치는 정도를 확인하기 위하여 세포활성도, 세포독성을 평가하고자 하였다. 일반치약 6종, 미백치약 3종, 천연치약 2종, 양성대조군으로 SLS(sodium lauryl sulfate)를 사용하였다. Immortalized human gingiva fibroblast cell 을 사용하여, 세포활성도 평가를 위하여 WST test, 세포 독성평가를 위해 Agar diffusion test를 시행하였다. 일반치약그룹, 미백치약그룹, 천연치약그룹 순으로 세포 생존률이 높게 나타났으며, Agar diffusion test는 일반치약그룹과 미백치약그룹은 높은 세포독성을 나타낸 반면 천연치약그룹은 낮은 독성을 나타냈다. 세포핵염색 결과 세포모양과 핵활성도 또한 천연치약이 가장 높은 활성도를 나타냈고, 미백치약, 일반치약 순으로 나타났다. 본 연구 결과, 치약의 사용목적에 따라 나누어지는 종류와 성분별 세포독성을 확인할 수 있었다. 소비자의 올바른 선택을 위해 치약의 자세한 성분표기가 필요할 것으로 사료된다.

This study was conducted to effect the cell activity and cytotoxicity of dentifrice. For the study, 6 kinds of general dentifrice, 3 kind of whitening dentifrice, 2 kinds of natural dentifrice and SLS(sodium lauryl sulfate) of positive control group. Immortalized human gingiva fibroblast cell was used for the study, WST test for cell activity and Agar diffusion test for cytotoxicity. Agar diffusion test showed high cytotoxicity in general dentifrice test group and whitening dentifrice test group, but low cytotoxicity in natural dentifrice test group. As a result of cell nucleus staining, cell shape and nuclear activity showed that the highest activity in natural dentifrice group, followed by whitening dentifrice group and general dentifrice group. As a result of this study, the cytotoxicity of different ingredient and according to the use to dentifrice. As a result of this study, we confirm cytotoxicity of kind and components according to the purpose of using dentifrice. Therefore, it is necessary to indicate the detailed ingredients of dentifrice for the smart choice of consumers.

키워드

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Fig. 1. Cell viability result of adults dentifrice * different alphabet was statistically significant difference

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Fig. 2. Result of cell morphology and nucleus activity (a) G-1 group, (b) G-2 group, (c) G-3 group, (d) G-4 group, (e) G-5 group, (f) G-6 group, (g) W-1 group, (h) W-2 group, (i) W-3 group, (j) N-1 group, (k) N-2 group (l) PC

Table 1. Appliance of dentifrice materials of this study

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Table 2. Decolorization index

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Table 3. Cell activity result of dentifrice

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Table 4. Agar diffusion assay zone grade result

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참고문헌

  1. B. Y. Kang et al. (2014). Preventive Dentistry, 5th edn., Koonja.
  2. B Cvikl & A Lussi,R Gruber. (2015). The in vitro impact of toothpaste extracts on cell viability. European Journal of Oral Science, 123, 179-185. https://doi.org/10.1111/eos.12177
  3. M. Fiume et al. (2010). Final Report on the Safety Assessment of Sodium Cetearyl Sulfate and Related Alkyl Sulfates as Used in Cosmetics. International Journal of Toxicology 29 (Supplement 2) 115S-132S. DOI: 10.1177/1091581810364665.
  4. H. G. Kwon et al. (2006). Primary Preventive Dentistry, 6th edn, Daehannarae, Seoul.
  5. G. C. Ryu, H. J. Park, J. M. Kim & J. B. Lee. (2001). Comparsion of protein removal effects and cytotoxicity in the L-929 cell line by tyloxapaol and tromthamine. The Korean Journal Vision Science, 3(1), 61-68.
  6. N. Evelyn, E. C. Daniela, K. V. Olav & C. J. Anne. (2007). Dual effects of sodium lauryl sulphate on human oral epithelial structure. Experimental Dermatology, 16(7), 574-579. https://doi.org/10.1111/j.1600-0625.2007.00567.x
  7. S. Jenkins, M. Addy & R. Newcome. (1991). Triclosan and sodium lauryl sulphate mouthrinses. (II). Effects of 4-day plaque regrowth. Journal of clinical Periodontol, 18, 145-148. https://doi.org/10.1111/j.1600-051X.1991.tb01704.x
  8. M. Fiume et al. (2010). Final report on the safety assessment of sodium cetearyl sulfate and related alkyl sulfates as used in cosmetics. Internationl Journal of Toxicology, May; 29 (3 Suppl), 115S-32S. DOI : 10.1177/1091581810364665.
  9. S. Marrakchi & H. I. Maibach. (2006). Sodium lauryl sulfate-induced irritation in the human face: regional and age-related differences. Skin Pharmacology and Physiology, 19(3), 177-180. DOI : 10.1159/000093112.
  10. B. B. Helofson & P. Barkwoll. (1996). Oral mucosal desquamation caused by two toothpaste detergents in an experimental model. European Journal of Oral Science, 104, 21-26. https://doi.org/10.1111/j.1600-0722.1996.tb00041.x
  11. A. B. Shaare, G. Rolla & P. Barkvoll (1997). The influence of triclosan, zinc or prophylene glycol on oral mucosa xposed to sodium lauryl sulphate. European Journal of Oral Science, 105, 527-533. https://doi.org/10.1111/j.1600-0722.1997.tb00240.x
  12. M. J. Choi, J. Y. Park, M. Y. Lim, J. Shuai, J. Heo, D. Y. Jung & H. S. Ryu. (2017). Factors affecting usage of toothpaste in infants and preschoolers. Journal Korean Society of Dental Hygiene, 17, 49-62. DOI :10.13065/jksdh.2017.17.01.49
  13. S. M. Levy, T. J. Maurice & J. R. Jakobsen. (1993). Dentifrice us among preschool children. Journal of American Dental Association. 124(9), 57-60. https://doi.org/10.14219/jada.archive.1993.0184
  14. R. P. Illeperuma et al. (2012). Immortalized gingival fibroblast as a cytotoxicity test model for dental materials. J. Mater. Sci. Mater. Med, 23, 753. https://doi.org/10.1007/s10856-011-4473-6
  15. International Standard Organization, ISO 10993-12. (2012). Biological Evaluation of Medical Devices-Part 12: Sample Preparation and Reference Materials.
  16. International Standard Organization, ISO 7405. (2008). Dentistry-Evaluation of Biocompatibility of Medical Devices Used in Dentistry.
  17. International Standard Organization, ISO 10993-5. (2009). Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity.
  18. N. Zhou, H. M. Wong & C. McGrath. (2019). Oral health and associated factors among preschool children with special health care needs. Oral Diseases. Feb 6. DOI : 10.1111/odi.13057. [Epub ahead of print]
  19. O. I. Karadaglioglu, N. Ulusoy, K.H.C. Baser & A. Hanoglu, I. Sik. (2019). Antibacterial Activities of Herbal Toothpaste Combined with Essential Oils against Streptococcus mutans. Pathogens, Feb 1;8(1), pii: E20. DOI : 10.3390/pathogens8010020.
  20. A. Green, S. Crichard, N. Ling-Mountford, M. Milward, N. Hubber, S. Platten, A.K. Gupta & I. L. C. Chapple. (2019). A randomised clinical study comparing the effect of Steareth 30 and SLS containing toothpastes on oral epithelial integrity (desquamation). Journal of Dentistry, Jan;80 Suppl 1, S33-S39. DOI : 10.1016/j.jdent.2018.11.005.
  21. J. Aramaki, I. Effendy, R. Happle, S. Kawana, C. Loffler & H. Loffler. (2001). Which bioengineering assay is appropriate for irritant patch testing with sodium lauryl sulfate? Contact Dermatitis, 45, 286-290. https://doi.org/10.1034/j.1600-0536.2001.450506.x
  22. B. L. Kuehl, K. S. Fyfe & N.H. Shear. (2003). Cutaneous cleansers. Skin therapy Letter, 8.
  23. E. Newburn, Dentifrices. In: Newburn A, ed. cariology. Chicago: Quintessence, 1989, 274-290.
  24. C. M. Healy, A. T. cruchley, M. H. Thornhill & D. M. W. illiams. (2000). the effect of sodium lauryl sulphate, triclosan and zincon the permeability of normal oral mucosa. Oral Disease. Mar, 6(1), 107-111.
  25. M. A. Jeong. (2010) Relationship of sodium lauryl sulfate content to the effects of dentifrice on halitosis. Korea Academy Industrial Cooperation Society, 5, 902-905.
  26. E Neppelberg, D. E. Costea, O. K. Vintermyr & A. C. Johannessen. (2007). Dual effects of sodium lauryl sulphate on human oral epithelial structure. Experimental dermatology, 16, 574-579. https://doi.org/10.1111/j.1600-0625.2007.00567.x
  27. S. O. Jang, Y. S. Shim & Y. R. Choi. (2016). Evaluation of cytotoxicity of child toothpaste. Science Advanced Materials, 8(2), 331-335. https://doi.org/10.1166/sam.2016.2489
  28. G. B. Nam, S. A. Cho, J. C. Cho, C. H. Kim, Y. J. Kim, J. H. Lee & K. H. Shin. (2012). The New in vitro Oral Irritation Test Method for Toothpaste using YD-38 Oral Mucosal Cell Line. Journal of the Society of Cosmetic Scientists of Korea, 38(4), 305-310. https://doi.org/10.15230/SCSK.2012.38.4.305
  29. H. Y. Jeong & Y. S. Kim. (2010). Variations of oral cavity environment according to sodium lauryl sulfate concentration of toothpaste. Journal of the Korea Contents Association, 10(8), 240-248. https://doi.org/10.5392/JKCA.2010.10.8.240
  30. J. L. Jensen & P. Barkvoll. (1998). Clinical implications of the dry mouth. Oral mucosal diseases Annals new york academy of sceince. Apr15, 842, 156-162. https://doi.org/10.1111/j.1749-6632.1998.tb09643.x
  31. J. W. Cho, S. C. Shin, Y. J. Jee, H. Y. Jung, J. H. Park & S. Y. Kang. (2006). Effect of Sodium Lauryl Sulfate Non-containing Dentifrice on Salivary Flow and Oral Malodor. Internal Journal of Clinlical Preventive Dentistry, 2(3), 178-188.
  32. M. H. Cho, R. Kim & S. H. Yu. (2017). A Research on Satisfaction of Toothpaste Added Natural Herbal Extract, The Journal of Korean academy of dental technology, 39(2), 111-118.
  33. S. B. Lee, B. H. Kim, H. R. Jung, S. H. Lee, H. J. Kwon, H. J. Bae & M. H. Yoon. (2016). Monitoring of Antimicrobial and Preservatives in Dentifrice. Journal of Food Hygiene and Safety, 31(4), 272-277. https://doi.org/10.13103/JFHS.2016.31.4.272