Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Quantitative Determination of Marker Compounds in the Extracts of Camellia sinensis L. Sub-branches (Residual Products) by HPLC

HPLC에 의한 차나무 잔가지(부산물)의 추출물 내 지표 성분의 정량분석

  • Lee, Min Sung (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Im, Hyeon Jeong (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Jeong, Hea Seok (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Cho, Hae Jin (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Woo, Hyun Sim (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Oh, Yu Jin (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Lee, Soo In (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Kim, Hyun Chul (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Ahn, Kyung Wan (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Kim, Yeong Su (Department of Forest Plant Industry, Baekdudaegan National Arboretum) ;
  • Kim, Dae Wook (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
  • 이민성 (국립백두대간수목원 산림식물산업부) ;
  • 임현정 (국립백두대간수목원 산림식물산업부) ;
  • 정회석 (국립백두대간수목원 산림식물산업부) ;
  • 조해진 (국립백두대간수목원 산림식물산업부) ;
  • 우현심 (국립백두대간수목원 산림식물산업부) ;
  • 오유진 (국립백두대간수목원 산림식물산업부) ;
  • 이수인 (국립백두대간수목원 산림식물산업부) ;
  • 김현철 (국립백두대간수목원 산림식물산업부) ;
  • 안경환 (국립백두대간수목원 산림식물산업부) ;
  • 김영수 (국립백두대간수목원 산림식물산업부) ;
  • 김대욱 (국립백두대간수목원 산림식물산업부)
  • Received : 2019.01.31
  • Accepted : 2019.02.25
  • Published : 2019.02.28
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Abstract

Background: Camellia sinensis L.(CS) is a perennial evergreen species of plant whose leaves are used to produce tea. In this plant species, the parts used are the leaves, sub-branch parts are thrown out. Methods and Results: Ethanol extract of sub-branch parts was used for isolation of major compounds by column chromatography. Structures were identified as caffeine (1), (-)-epicatechin (2) and (-)-epicatechin gallate (3) by interpretation of spectroscopic analysis, including $^1H$- and $^{13}C$-NMR. High-performance liquid chromatography (HPLC) method was used to compare the quantitative level of marker compounds in various extraction solvents of sub-branch parts of CS. The content of caffeine, (-)-epicatechin, and (-)-epicatechin gallate in 30% ethanol extract showed higher value with $3.28{\pm}0.57mg/g$, $5.53{\pm}0.88mg/g$, and $1.29{\pm}0.24mg/g$, respectively. Conclusions: These results indicated that not only leaves parts but also sub-branch, could be a good source for the functional material and pharmaceutical industry.

Keywords

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Fig. 1. 1H-NMR spectrum and structure of compounds isolated from C. sinensis L. branches.

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Fig. 2. Analytical HPLC chromatogram of isolated compounds.

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Fig. 3. Calibration curves for caffeine (■), (−)-epicatechin (▲) and (−)-epicatechin gallate (●).

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Fig. 4. HPLC chromatogram (absorbance at 280㎚) from Camellia sinensis L. branches extracted with different solvents.

Table 1. Calibration data for analysis of marker compounds (1 − 3) in of Camellia sinensis L. sub-branches.

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Table 2. Content of marker compounds of extract of Camellia sinensis L. sub-branches at different solvent.

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