Browse > Article
http://dx.doi.org/10.7783/KJMCS.2019.27.1.24

Quantitative Determination of Marker Compounds in the Extracts of Camellia sinensis L. Sub-branches (Residual Products) by HPLC  

Lee, Min Sung (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Im, Hyeon Jeong (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Jeong, Hea Seok (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Cho, Hae Jin (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Woo, Hyun Sim (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Oh, Yu Jin (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Lee, Soo In (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Kim, Hyun Chul (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Ahn, Kyung Wan (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Kim, Yeong Su (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Kim, Dae Wook (Department of Forest Plant Industry, Baekdudaegan National Arboretum)
Publication Information
Korean Journal of Medicinal Crop Science / v.27, no.1, 2019 , pp. 24-29 More about this Journal
Abstract
Background: Camellia sinensis L.(CS) is a perennial evergreen species of plant whose leaves are used to produce tea. In this plant species, the parts used are the leaves, sub-branch parts are thrown out. Methods and Results: Ethanol extract of sub-branch parts was used for isolation of major compounds by column chromatography. Structures were identified as caffeine (1), (-)-epicatechin (2) and (-)-epicatechin gallate (3) by interpretation of spectroscopic analysis, including $^1H$- and $^{13}C$-NMR. High-performance liquid chromatography (HPLC) method was used to compare the quantitative level of marker compounds in various extraction solvents of sub-branch parts of CS. The content of caffeine, (-)-epicatechin, and (-)-epicatechin gallate in 30% ethanol extract showed higher value with $3.28{\pm}0.57mg/g$, $5.53{\pm}0.88mg/g$, and $1.29{\pm}0.24mg/g$, respectively. Conclusions: These results indicated that not only leaves parts but also sub-branch, could be a good source for the functional material and pharmaceutical industry.
Keywords
Camellia sinensis L.; Sub-branch; Marker Compounds; HPLC; Quantitative Analysis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Choi SH and Rhyu MR. (1992). Analysis of theanine contents in commercial green tea. Korean Journal of Food Science and
2 Chung DH. (2004). Components and efficacy of tea. Hongikjae. Seoul, Korea. p.43-46.
3 Crespy V and Williamson G. (2004). A review of the health effects of green tea catechins in vivo animal models. Journal of Nutrition. 134:3431-3440.   DOI
4 Goto T, Yoshida Y, Kiso M and Nagashima H. (1996). Simultaneous analysis of individual catechins and caffeine in green tea. Journal of Chromatography A. 749:295-299.   DOI
5 Jung E, Lee J, Baek J, Jung K, Lee J, Huh S, Kim S, Koh J and Park D. (2007). Effect of Camellia japonica oil on human type I procollagen production and skin barrier function. Journal of Ethnopharmacology. 112:127-131.   DOI
6 Kasaoka S, Hase K, Morita T and Kiriyama S. (2002). Green tea flavonoids inhibit the LDL oxidation in osteogenic disordered rats fed a marginal ascorbic acid in diet. Journal of Nutritional Biochemistry. 12:96-102.
7 Kerio LC, Wachira FN, Wanyoko JK and Rotich MK. (2013). Total polyphenols, catechin profiles and antioxidant activity of tea products from purple leaf coloured tea cultivars. Food Chemistry. 136:1405-1413.   DOI
8 Khan MI, Ahhmed A, Shin JH, Baek JS, Kim MY and Kim JD. (2018). Green tea seed isolated saponins exerts antibactieral effects against various strains of gram positive and gram negative bacteria, a comprehensive study in vitro and in vivo. Evidence-Based Complementary and Alternative Medicine. 2018:3486106. https://www.hindawi.com/journals/ecam/2018/3486106/ (cited by 2019 Jan 31).   DOI
9 Kim JD, Lee BI, Jeon YH, Bak JP, Jin HL and Kim BO. (2010). Anti-oxidative and anti-inflammatory effects of green tea mixture and dietary fiber on liver of high fat diet-induced obese rats. Korean Journal of Medicinal Crop Science. 18:224-230.
10 Kim JT. (1996). Science and culture of tea. Borim press. Seoul, Korea. p.160.
11 Kim NY, Lee JH and Heo MY. (2006). Protective effects of green tea extracts on oxidative stress. Korean Journal of Medicinal Crop Science. 14:322-328.
12 Kim SH, Han DS and Park JD. (2004). Changes of some chemical compounds of Korean(Posong) green tea according to harvest periods. Korean Journal of Food Science and Technology. 36:542-546.
13 Kwon YM, Lee JH and Lee MW. (2002). Phenolic compounds from barks of Ulmus macrocarpa and Its antioxidative activities. Korean Journal of Pharmacognosy. 33:404-410.
14 Lee LS, Kim SH, Kim YB and Kim YC. (2014). Quantitative analysis of major constituents in green tea with different plucking periods and their antioxidant activity. Molecules. 19:9173-9186.   DOI
15 Mukherjee PK, Maity N, Nema NK and Sarkar BK. (2011). Bioactive compounds from natural resources against skin aging. Phytomedicine. 19:64-73.   DOI
16 Park YG. (2007). Strategy of gene conservation of Camellia sinensis in Korea. Journal of Korean Tea Society. 13:125-140.
17 Oda Y, Hidaka M and Suzuki A. (2017). Caffeine has a synergistic anticancer effect with cisplatin via inhibiting fanconi anemia group D2 protein monoubiquitination in hepatocellular carcinoma cells. Biological and Pharmaceutical Bulletin. 40:2005-2009.   DOI
18 Oh MJ and Hong BH. (1995). Variation of pectin, catechins and caffein contents in Korean green tea(Camellia sinensis L.) by harvesting time and processing recipe. Korean Journal of Crop Science. 40:775-781.
19 Okumura T, Tsukui T, Hosokawa M and Miyashita K. (2012). Effect of caffeine and capsaicin on the blood glucose levels of obese/diabetic $KK-A^y$ mice. Journal of Oleo Science. 61:515-523.   DOI
20 Park JH and Lim KC. (2002). Growth and constituents of tea shoots for powder green tea. Korean Journal of Medicinal Crop Science. 10:379-383.
21 Srtkowski J, Stefaniak L, Nicol L, Martin ML, Martin GJ and Webb GA. (1995). Complete assignments of the $^{1}H$, $^{13}C$ and $^{15}N$ NMR spectra of caffeine. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 51:839-841.   DOI
22 Vermeer MA, Mulder TP and Molhuizen HO. (2008). Theaflavins from black tea, especially theaflavin-3-gallate, reduce the incorporation of cholesterol into mixed micelles. Journal of Agricultural and Food Chemistry. 56:12031-12036.   DOI
23 Watandabe M. (1998). Catechins as antioxidants from Buckwheat (Fagopyrum esculentum Moench) groats. Journal of Agricultural and Food Chemistry. 46:839-845.   DOI
24 Wee JH, Sung HM, Jung KO, Kim SJ, Shin YR, Park JH and Kim JD. (2015). Anti-adipogenic effects of the water extracts of defatted green tea seed cake. Korean Journal of Food Science and Technology. 47:525-533.   DOI
25 Xu J, Wang M, Zhao J, Wang YH, Tang Q and Khan IA. (2018). Yellow tea(Camellia sinensis L.), a promising Chinese tea: Processing, chemical constituents and health benefits. Food Research International. 107:567-577.   DOI
26 Yoon WH, Choi JH, Lee KH and Kim CH. (2005). Antimicrobial and antitumor activities of seed extracts of Camellia sinensis L. Korean Journal of Food Science and Technology. 37:108-112.