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The Effects of Korea Ginger Essential oil on Chronic Mild Stress-induced Rats Model of Sympathetic Hyperactivity

국내 생강 에센셜오일이 만성 스트레스로 교감신경이 항진된 동물 모델에 미치는 영향

  • Ji, Joong-Gu (Division of Integrated Biotechnology, Joongbu University)
  • 지중구 (중부대학교 바이오융합학부)
  • Received : 2019.12.03
  • Accepted : 2019.12.26
  • Published : 2019.12.30

Abstract

Exposure to stress can lead to sympathetic hyperactivity, which include a systemic disease with lesions in a broad spectrum of organs and a psychiatric condition. The purpose aimed to evaluate the effect of the korea ginger essential oil on stress hormone and brain tissue response in chronic mild stress-induced rats model of sympathetic hyperactivity. Evaluation method carried out cytotoxicity assessment and component analysis, what observe the effects based on serum biomarker and histopathological analysis of brain tissue. As this time, the korea ginger essential oil was treatment at doses of 100 nl/㎖ for 2 weeks after make a chronic mild stress-induced rats model of sympathetic hyperactivity. As a result, the korea ginger essential oil was not toxic at less than 100 nl/㎖, contents of 6-gingerol was 345 ppm. Compared to the control rats, the korea ginger essential oil treatments significantly reduced the serum concentrations of stress hormone (i.e., ACTH, corticosterone, melatonin). Furthermore, The korea ginger essential oil treatments effectively preserved the TH-immune response was occasionally interrupted in the ventral tegmental area (VTA) and the substantia nigra pars compacta (SNpc). The results indicate that the korea ginger essential oil improved sympathetic hyperactivity. Thus, the korea ginger may be a novel material of aroma oils for the management of sympathetic hyperactivity.

스트레스는 인체의 다양한 기관과 정신적 상태에 영향을 미치는 교감신경 항진을 야기시킨다. 본 연구의 목적은 만성 스트레스로 교감신경을 항진시킨 동물 모델에서 국내산 생강 에센셜오일이 스트레스 호르몬 및 뇌 조직 반응에 미치는 효과를 평가하였다. 평가 방법은 세포독성 평가 및 성분 분석을 수행하였으며, 혈청 바이오 마커와 뇌 조직의 병리학적 분석이 기초한 효과를 관찰하였다. 동물 실험에서 국산 생강 에센셜오일의 처리는 만성 스트레스로 교감신경을 항진시킨 동물 모델 제작 후 2주간 100 nl/㎖로 처리하였다. 그 결과, 국산 생강 에센셜오일은 100 nl/㎖ 농도 이하에서 독성이 없었으며, 6-진저롤 함량이 345 ppm으로 확인되었다. 국산 생강 에센셜오일의 처리는 대조군과 비교하여 혈청에서 부신피질호르몬, 코르티코스테론, 멜라토닌과 같은 스트레스 호르몬의 농도를 크게 줄였으며, 복측피개부(VTA) 및 흑색질 치밀부(SNpc) 부분에서 TH-면역 반응이 때때로 중단되는 것을 효과적으로 보존하였다. 이와 같은 결과는 국산 생강 에센셜오일이 교감신경 항진을 개선했음을 나타내고 있다. 따라서 국산 생강은 교감신경 항진에 대한 아로마오일의 새로운 원료로 활용될 수 있다.

Keywords

References

  1. D. H. Kim, "Effect of Aromatherapy on the autonomic nervous system", Journal of the Korea Academia-Industrial cooperation Society, Vol.20, No.6, pp. 261-271, (2019). https://doi.org/10.5762/KAIS.2019.20.6.261
  2. S. W Lee, "A Study on the Effects of the hospitalization stress on the sleep pattern", Journal of Korean Academy of Nursing, Vol.16, No.2, pp.36-43, (1986).
  3. S. H. Park, Y. J. Lee, "Analysis of antipsychotic induced concomitant prescribing of antiparkinson agents in Korea", Korean Journal of Clinical Pharmacy, Vol.22, No.2, pp. 137-142, (2012).
  4. Grand view research. Essential oils market size, share & trends analysis report by application (cleaning & home, medical, food & beverages, spa & relaxation), by product, by sales channel, and segment forecasts, 2019-2025. p.3. Market research report, (2019).
  5. I. R. Bae, J. A. Song, M. Lee, M. Hur, "Effects of aromatherapy essential oil inhalation on the stress response after exposure to noise and arithmetic subtraction stressor: randomized controlled trial", International Journal of Clinical and Experimental Medicine, Vol.11, No.1, pp. 275-284. (2018).
  6. S. J. Ryu, M. S. Lee, "Effect of applying the negative.positive essential oil back massage on the autonomic nervous system : for 30-year old housewives", Journal of the Korean Society of Cosmetology, Vol.23, No.5, pp. 903-909, (2017).
  7. J. Y. Kwak, H. J. Choi, J. M. Park, J. H. Park, Y. M. Kho, T. S. Jang, T. Ahn, "Anti-atopic effect of Zizyphus jujuba var. spinosa composite oil (ZjJ-C_oil) and Aralia cordata var. continentalis composite oil (ARC-C_oil) in DNCB-induced atopic dermatitis-like skin lesions NC/Nga Mice", Journal of Sasang Constitutional Medicine, Vol.29, No.4, pp. 347-368, (2017). https://doi.org/10.7730/JSCM.2017.29.4.347
  8. S. H. Han, Y. J. Ro, M. H. Hur, "Effects of aromatherapy on menstrual cramps and dysmenorrhea in college student woman : a blind randomized clinical trial", Korean Journal of Adult Nursing, Vol.13, No.3, pp. 420-430, (2001).
  9. S. Battaglia, The complete guide to aromatherapy, pp. 303-308, The International Centre of Holistic Aromatherapy, (2003).
  10. T. S. Lim, O. J. Kwon, J. H. Kwon, H. K. Kim, "Monitoring of extraction yields and functional properties of ginger (Zingiber officinale) extracts using response surface methodology", Journal of the Korean Society of Food Science and Nutrition, Vol.36, No.3, pp. 348-354, (2007). https://doi.org/10.3746/jkfn.2007.36.3.348
  11. M. H. Jo, I. K. Ham, G. H. Lee, J. K. Lee, G. S. Lee, S. K. Park, T. I. Kim, E. M. Lee, "Comparison of active ingredients between field grown and in vitro cultured rhizome of Korean native ginger (Zingiber officinale Roscoe)", Korean Journal of Plant Resources, Vol.24, No.4, pp. 404-412. (2011). https://doi.org/10.7732/kjpr.2011.24.4.404
  12. J. P. Bartley, A. L. Jacobs, "Effects of drying flavour compounds in Australiangrowth ginger (Zingiber officinale)", Journal of the Science of Food and Agriculture, Vol.80, No.2, pp. 209-215, (2000). https://doi.org/10.1002/(SICI)1097-0010(20000115)80:2<209::AID-JSFA516>3.0.CO;2-8
  13. T. A. V. Beek, G. P. Lelyveld, "Isolation and identification of the five major sesquiterpene hydrocarbons of ginger", Phytochemical Analysis, Vol.2, No.1, pp. 26-34, (1991). https://doi.org/10.1002/pca.2800020106
  14. M. H. Park. "Study on the drying processes and extraction methods of ginger and the application of anti-oxidative substance", Sungshin women's University, (2014).
  15. C. K. Park, B. S. Jeon, J. W. Yang, "The chemical compounds of Korean ginseng", Food Industry and Nutrition, Vol.8, No.2, pp. 10-23, (2003).
  16. S. H. Choi, O. T. Seung, M. S. Lee, "Improving effect to connitive ability of Cordyceps militaris extract in PC12 and BV2 cells", Journal of the Korean Applied Science and Technology, Vol.36, No.2, pp. 468-478, (2019). https://doi.org/10.12925/JKOCS.2019.36.2.468
  17. H. C. Kim, Chinese Pharmacology, pp. 86-88, Jipmoon, (2001).
  18. H. J. Kim, "The effects of different types of exercise on ACTH, cortisol and catecholamine : with focus on the male elders", Hanyang University, (1998).
  19. N. E. Bae, "The effects of the sound pressure in background music on the fatigue factors secretion during aerobic exercise", Journal of Korean Physical Education Association for Girls and Women, Vol.22. No.5. pp. 27-36, (2008).
  20. G. C. An, "The effect of aroma-fragrance necklace on stress, anxiety and physiological response in hemodialysis patients", Gongju University. (2012).
  21. W. K. Choi, K. S. Lee, H. Y. Joung, Y. C. Lee, J. H. Sohn, B. H. Lee, K. H. Pyun, I. S. Shim, "Low frequency noise induces stress responses in the rat", Korean Journal of the science of Emotion and sensibility, Vol.10, No.3, pp. 411-418, (2007).
  22. S. Y. Lee, J. H. Choi, H. S. Jeong, Y. G. Kim, S. I. Cho, "Piperis Nigri Fructus Extract Ameliorates Psychological Stress in Mice", Journal of Haehwa Medicine, Vol.21, No.2, pp. 95-104, (2013).
  23. Y. J. Ko, M. S. Jung, K. S. Park, "Effects of aroma inhalation method on test anxiety, stress response and serum cortisol in nursing students", Journal of Korean Academy of Fundamentals of Nursing, Vol.20, No.4, pp. 410-418, (2013). https://doi.org/10.7739/jkafn.2013.20.4.410
  24. S. J. Hong, E. H. Kim, "Effects of aroma head and neck massage on sleep disturbance and problematic behaviors, depression, blood serotonin, blood cortisol, and the vital signs on elders with dementia", Crisisonomy, Vol.10, No.9, pp. 75-92, (2014).
  25. I. S. Kim, S. J. Kang, J. O. Kim, "Effects of the aroma inhalation method with a roll-on on life stress, salivary cortisol and fatigue in nursing student", Journal of the Korea Academia-Industrial cooperation Society, Vol.15.No.12, pp. 7214-7223, (2014). https://doi.org/10.5762/KAIS.2014.15.12.7214
  26. G. F. KOOB, "Corticotropin-releasing factor, norepinephrine, and stress", Biological psychiatry, Vol.46. No.9, pp. 1167-1180, (1999). https://doi.org/10.1016/S0006-3223(99)00164-X
  27. A. G. LEVENTHAL, Y. Wang, M. Pu, Y. Zhou, Y. Ma, "GABA and its agonists improved visual cortical function in senescent monkeys". Science, Vol.300, No.5620, pp. 812-815, (2003). https://doi.org/10.1126/science.1082874
  28. J. S. Lee, J. Y. Lee, W. G. Cho, Y. C. Yang, B. P. Cho, "Relationship between Microglial Activation and Dopaminergic Neuronal Loss in 6-OHDA-induced Parkinsonian Animal Model". Korean Journal of Physical Anthropology, Vol.26, No.1, pp. 13-23, (2013). https://doi.org/10.11637/kjpa.2013.26.1.13