Journal of Industrial and Engineering Chemistry

  • Volume 67
  • /
  • Pages.80-91
  • /
  • 2018
  • /
  • 1226-086X(pISSN)
  • /
  • 1876-794X(eISSN)
The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Agglomeration of human dermal fibroblasts with ECM mimicking nano-fragments and their effects on proliferation and cell/ECM interactions

  • Received : 2018.05.01
  • Accepted : 2018.06.11
  • Published : 2018.11.25
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Abstract

Here, we engineered spheroids by using ECM mimicking nano-fragments (NFs) with fibroblasts and investigated their effect on proliferation and cell/ECM interactions. NF incorporation resulted in formation of a stable spheroid, which improved proliferation and viability of cells by assisting oxygen transport confirmed by LOX-1 staining. In addition, hypoxic and apoptotic genes were significantly downregulated in spheroids with PD-NFs. Furthermore, ECM and cell junction proteins were highly expressed. Overall, our findings suggest that incorporation of NFs within spheroids for assembly with various cell types can be an alternative approach for 3D cell culture in many applications.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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