Korean Journal of Chemical Engineering

The Korean Institute of Chemical Engineers (한국화학공학회)
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An organofunctionalized MgO∙SiO2 hybrid support and its performance in the immobilization of lipase from Candida rugosa

  • Kolodziejczak-Radzimska, Agnieszka (Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology) ;
  • Zdarta, Jakub (Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology) ;
  • Ciesielczyk, Filip (Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology) ;
  • Jesionowski, Teofil (Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology)
  • Received : 2018.05.07
  • Accepted : 2018.08.28
  • Published : 2018.11.30
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Abstract

Lipase from Candida rugosa was immobilized on $MgO{\cdot}SiO_2$ hybrid grafted with amine, thiol, cyano, phenyl, epoxy and carbonyl groups. The products were analyzed using Fourier transform infrared spectroscopy, nuclear magnetic resonance, low-temperature $N_2$ sorption and elemental analysis. Additionally, the degree of coverage of the oxide material surface with different functional groups and the number of surface functional groups were estimated. The Bradford method was used to determine the quantity of immobilized enzyme. The largest quantity of enzyme (25-28 mg/g) was immobilized on the hybrid functionalized with amine and carbonyl groups. On the basis of hydrolysis reaction of p-nitrophenyl palmitate to p-nitrophenol, it was determined how the catalytic activity of the obtained biocatalysts is affected by pH, temperature, storage time, and repeated reaction cycles. The best results for catalytic activity were obtained for the lipase immobilized on $MgO{\cdot}SiO_2$ hybrids with amine and carbonyl groups. The biocatalytic system demonstrated activity above 40% in the pH range 4-10 and in the temperature range $30-70^{\circ}C$. Lipase immobilized on the $MgO{\cdot}SiO_2$ systems with amine and epoxy groups retains, respectively, around 80% and 60% of its initial activity after 30 days of storage, and approximately 60-70% after 10 reaction cycles.

Keywords

Acknowledgement

Supported by : Poznan University of Technology

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