Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Effect of Malonic Acid-Catalyzed Pretreatment on the Hydrolysis of Gracilaria verrucosa

Malonic acid를 이용한 전처리가 꼬시레기의 가수분해에 미치는 영향

  • Park, Mi-Ra (Department of Biotechnology, Pukyong National University) ;
  • Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University)
  • 박미라 (부경대학교 생물공학과) ;
  • 정귀택 (부경대학교 생물공학과)
  • Received : 2018.05.25
  • Accepted : 2018.06.29
  • Published : 2018.08.01
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Abstract

In this study, the effects of malonic acid-catalyzed pretreatment on the subsequent enzymatic hydrolysis of red macro-algae Gracilaria verrucosa for production of biosugar (total reducing sugar) were investigated. In the hydrothermal pretreatment condition of 300 mM malonic acid, 1:20 solid-to-liquid ratio at $130^{\circ}C$ for 60 min, a 49.2% biosugar yield was achieved. Moreover, by subsequent enzymatic hydrolysis after pretreatment, maximum yield of 64.5% was achieved.

본 연구에서는 해양 거대조류 중 홍조류에 속하는 꼬시레기(Gracilaria verrucosa)로부터 malonic acid를 사용하여 열수 전처리 조건(전처리 온도, 촉매 농도, 고액비, 전처리 시간)에 따른 전처리와 효소가수분해를 통한 바이오슈거(환원당) 생산 가능성을 조사하였다. 300 mM malonic acid, 1:20 고액비 조건에서 $130^{\circ}C$에서 60분간 전처리를 수행하여 49.2%의 환원당 수율을 얻었다. 전처리 반응 후 이어진 효소 가수분해를 통하여 64.5%의 수율을 확보하였다.

Keywords

References

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