Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidant and Anti-amyloid Activities of Fermented Kalopanax pictus

엄나무 발효물의 항산화 및 항아밀로이드 활성

  • 강정훈 (청주대학교 바이오메디컬학과)
  • Received : 2018.05.29
  • Accepted : 2018.06.27
  • Published : 2018.06.30
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Abstract

This study was to investigate the antioxidant and anti-amyloid activities of the extract (KP-HE) from Kalopanax pictus (KP) fermented with Hericium erinaceum (HE) mycelium. Antioxidant activity was evaluated based on 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical(ABTS) scavenging assays. In all assays, the extracts from KP, HE and KP-HE had the potential for antioxidant activities. However, antioxidant activity of KP-HE significantly scavenged DPPH radical as compared to the KP and HE. The result suggested that the antioxidant component was increased in the process of KP fermented with HE. KP-HE was shown to significantly inhibite peroxyl radical-mediated DNA strand breakage whereas KP and HE did not inhibit DNA strand breakage. The aggregation of the amyloid-${\beta}$ ($A{\beta}$) peptide is involved in the pathological process of Alzheimer's disease(AD). In this study, the effects of KP, HE and KP-HE on the aggregation of $A{\beta}_{1-42}$ were investigated. KP and HE had little effect on $A{\beta}$ aggregation and KP-HE effectively inhibited $A{\beta}$ aggregation. KP-HE effectively inhibited $A{\beta}$ induced cell death and significantly increased of the 20.3% cell survival at $300{\mu}g/mL$ concentration. KP-HE also decreased intracellular reactive oxygen specie levels in $A{\beta}$-treated cells. The results suggested that KP-HE had antioxidant and anti-amyloid activities. Therefore, KP-HE could potentially be used as a valuable functional food ingredient to prevent neurodegenerative disorders such as AD.

본 연구는 노루궁뎅이버섯 균사체로 발효시킨 엄나무 추출물의 항산화 및 항아밀로이드 활성을 알아보고자 하였다. 항산화 활성은 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radical 소거 측정법을 사용하여 관찰하였다. 엄나무추출물(KP), 노루궁뎅이버섯 균사체 추출물(HE), 엄나무 발효물(KP-HE)에서 모두 라디칼 소거활성이 관찰되었다. 그러나 KP-HE가 KP와 HE에 비해서 더 높은 소거 활성을 갖는 것으로 관찰되었다. KP-HE는 peroxyl radical에 의한 DNA의 산화적 손상을 억제하였다. 알츠하이머병 (Alzheimer's disease: AD)과 관련 있는 $A{\beta}_{1-42}$의 응집에 KP, HE, KP-HE가 어떤 영향을 미치는 지를 알아보았다. KP와 HE는 $A{\beta}_{1-42}$의 응집에 거의 영향을 미치지 않았고 KP-HE는 $A{\beta}_{1-42}$의 응집을 효과적으로 억제하였다. 또한 $A{\beta}_{1-42}$에 의한 신경세포 사멸에 엄나무 발효물을 $300{\mu}g/mL$ 농도로 전 처리한 세포생존율은 20.3% 높게 증가되었다. 또한 엄나무 발효물을 $50{\mu}g/mL$ 농도로 처리했을 경우 세포 내 ROS의 축적이 유의적으로 감소되었다. 결론적으로 본 연구에서 관찰된 결과들을 통해 엄나무 발효물은 항산화 및 항아밀로이드 활성을 가지는 것으로 확인되었다. 따라서 엄나무 발효물은 알츠하이머병과 같은 퇴행성 뇌질환을 예방할 수 있는 식품소재로 이용될 수 있을 것으로 사료된다.

Keywords

References

  1. A. Lorenzo, B. A. Yankner, "Amyloid fibril toxicity in Alzheimer's disease and diabetes", Ann. N Y Acad. Sci., Vol.17, No.1 pp. 89-95, (1996).
  2. C. Glabe, "Intracellular mechanisms of amyloid accumulation and pathogenesis in Alzheimer's disease", J. Mol. Neurosci., Vol.17, No.2 pp. 137-45, (2001). https://doi.org/10.1385/JMN:17:2:137
  3. P. Domenico, "Alzheimer's disease and oxygen radicals: new insights", Biochemica.l Pharmacology, Vol.63, No.6 pp. 563-567, (2002). https://doi.org/10.1016/S0006-2952(01)00919-4
  4. C. Behl, "Oxidative stress in Alzheimer's disease: implications for prevention and therapy", Subcell. Biochem., Vol.38, pp. 65-78, (2005).
  5. H. Kim, S. J. Yoon, K. H. Lee, H. J. Kwon, S. S. Chun, T. W. Kim, Y. J. Cho, "Screening of biological activitis of the extracts fro Basil (Ocimun basilicum L.)", J. Korean Soc. Appl. Biol. Chem., Vol.48, No.2 pp. 173-177, (2005).
  6. H. J. Im, H. L. Jang, Y. J. Jeong, K. Y. Yoon, "Chemical properties and antioxidant activities of the sprouts of Kalopanax pictus, Cedrela sinensis, Acanthopanax cortex at different plucking times", Korean J. Food Preserv., Vol.20, No.3 pp. 356-364, (2013). https://doi.org/10.11002/kjfp.2013.20.3.356
  7. J. D. Hwang, J. S. Choi, J. B. Kim, S. L. Yang, "Antioxidant activities of bark extracts from Kalopanax picuts", J. Invest. Cosmet., Vol.7, No.4 pp. 329-337, (2011). https://doi.org/10.15810/jic.2011.7.4.001
  8. H. Kawagishi, A. Shimada, K. Shizuki, H. Mori, H. Sakamoto, S. Furukawa, E. F. "Erinacines, Stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum", Tetrahedron Lett., Vol.41, No.2 pp. 7399-7402, (1996).
  9. S. H. Park, J. S. Chang, K. R. Lee, "Effect of Hericium erinaceius extract on cancer cell growth and expression of cell cycle associated proteins", J. Korean Soc. Food Sci. Nutr., Vol.32, No.6, pp. 931-936, (2003). https://doi.org/10.3746/jkfn.2003.32.6.931
  10. Y. S. Kim, M. Y. Joung, B. S. Ryu, P. J. Park, J. H. Jeong, "Anti-inflammatory activities of extracts from fermented Taraxacum platycarpum D. leaves using Hericium erinaceum mycelia", J. Korean Soc. Food Sci. Nutr., Vol.45, No.1 pp. 20-26, (2016). https://doi.org/10.3746/jkfn.2016.45.1.020
  11. H. Kim, J. Y. Shin, A. R. Lee, J. H. Hwang, K. W. Yu, "Physiological avtivity of thye fermented small atate culture of the beared tooth mushroom (Hericium erinaceum) mycelia" Korean J. Food Nutr., Vol.30, No.6 pp. 1348-1356, (2017). https://doi.org/10.9799/KSFAN.2017.30.6.1348
  12. W. Brand-Williams, M. E. Cuvelier, C. Berset, "Use of a free radical method to evaluate antioxidant activity", Food Sci. Tech.., Vol.28, No.1 pp. 25-30, (1995).
  13. J. H. Kang, S. M. Kim, "DNA cleavage by hydroxyl radicals generated in the Cu, Zn-superoxide dismutase and hydrogen peroxide system", Mol. Cells, Vol.7, No.6 pp. 777-782, (1997).
  14. K. S. Kim, S. Y. Choi, H. Y. Kwon, M. H. Won, T. C. Kang, J. H. Kang, "Aggregation of alpha-synuclein induced by the Cu,Zn-superoxide dismutase and hydrogen peroxide system", Free Radic. Biol. Med., Vol.15, No.6 pp. 544-550, (2002).
  15. E. H. Ahn, D. W. Kim, M. J. Shin, Y. N. Kim, H. R. Kim, S. J. Woo, S. M. Kim, D. S. Kim, J. Kim, J. Park, W. S. Eum, H. S. Hwang, S. Y. Choi, "PEP-1-ribosomal protein S3 protects dopaminergic neurons in an MPTP-induced Parkinson's disease mouse model", Free Radic. Biol. Med., Vol.55, pp. 36-45, (2013). https://doi.org/10.1016/j.freeradbiomed.2012.11.008
  16. S. H. Kim, Y. Park, Y. S. Jang, J. Han, H. G. Chung, "Oxidative stress in the cell and antioxidant activity of Kalopanax pictus extracts", J. Korean Wood Sci. Tech., Vol.35, No.6 pp. 26-143, (2007).
  17. K. Hermann, "Occurrence and content of hydroxycinnamic and hydroxylbenzoic acid compounds in foods", Critical Rev. Food Sci. Nut., Vol.28, No.4 pp. 315-347, (1989). https://doi.org/10.1080/10408398909527504
  18. S. I. Heo, H. J. Jung, M. K. Kim, M. H. Wang, "Antioxidative activities and tyrosinase inhibitory effects of Korean medicianl plants", J. Appl. Biol. Chem., Vol.50, No.3 pp. 115-119, (2007).
  19. M. H. Kim, S. Y. Park, Y. J. Jeong, K. Y. Yoon, "Sensory properties of Kalopanax pictus and Cedrela sinensis shoots under different blanching conditions and with different thawing methods", Korean J. Food Preserv., Vol.19, No.2 pp. 201-208, (2012). https://doi.org/10.11002/kjfp.2012.19.2.201
  20. Y. M. Lee, J. H. Bae, H. Y. Jung, J. H. Kim, D. S. Park, "Antioxidant activity in water and methanol extracts from Korean edible wild plants", J. Korean Soc. Food Sci. Nutr., Vol.40, No.1 pp. 29-36, (2011). https://doi.org/10.3746/jkfn.2011.40.1.029
  21. D. H.Jun, J. T. Lee, S. J. Cheon, C. E. Lee, T. H. Kim, D. H. Lee, J. Han, S. Y. Kim, "Polyphenol and anti-oxidant effects of Kalopanax septemlobus Koidz. leaf extracts", Korean J. Plant Res., Vol.22, No.4 pp. 343-348, (2009).
  22. B. Halliwell, J. M. Gutterridge, "Roles of free radicals and catalytic metal ions in human disease: An overreview", Methods Enzymol., Vol.186, pp. 1-85, (1990).
  23. D. J. Selkoe, "Translating cell biology into therapeutic advances in Alzheimer's disease", Nature, Vol.399, pp. A23-A31, (1999). https://doi.org/10.1038/399a023
  24. Y. Gong, L. Chang, K. L. Viola, P. N. Lacor, M. P. Lambert, C. E. Finch, G. A. Krafft, W. L. Klein, "Alzheimer's disease-affected brain: presence of oligomeric A-beta ligands(ADDLs) suggests a molecular basis for reversible memory loss", Proc. Natl. Acad. Sci. USA., Vol.100, No.18 pp. 10417-10422, (2003). https://doi.org/10.1073/pnas.1834302100
  25. C. Behl, "Alzheimer's disease and oxidative stress: implications for novel therapeutic approaches", Prog. Neurobiol., Vol.57, No.3 pp. 301-429, (1999). https://doi.org/10.1016/S0301-0082(98)00055-0
  26. C. B. Pocernich, D. A. Butterfield, "Elevation of glutathione as a therapeutic strategy in Alzheimer disease", Biochem. Biophys. Acta, Vol.1822, No.5 pp. 625-630, (2012).
  27. D. A. Butterfield, C. M. Lauderback, "Lipid peroxidation and protein oxidation in Alzheimer's disease brain: potential causes and consequences involving amyloid beta-peptide-associated free radical oxidative stress", Free Radic. Biol. Med., Vol.32, No.11 pp. 1050-1060, (2002). https://doi.org/10.1016/S0891-5849(02)00794-3
  28. O. Vergun, A. I. Sobolevsky, M. V. Yelshansky, J. Keelan, B. I. Khodorov, M. R. Duchen, "Exploration of the role of reactive oxygen species in glutamate neurotoxicity in rat hippocampal neurones in culture", J. Physiol., Vol.531, No.1 pp. 147-163, (2001). https://doi.org/10.1111/j.1469-7793.2001.0147j.x