Figure 1. Effect of sulforaphane, PF-3758309 or sulforaphane+ PF-3758309 on cell viability in RAW 264.7 cells. RAW 264.7 cells (1 × 104) were seeded into 96-well plates and cultured for 24 h. Cells were then treated with increasing concentrations of sulforaphane (SFN) (A), PF -3758309 (PF) (B) or the indicated concentrations of sulforaphane+PF-3758309 (C) for 24 h. Cell viability was measured by the MTT assay.
Figure 3. Effect of sulforaphane, PF-3758309 or sulforaphane+ PF-3758309 on melanin synthesis in B16F10 cells. B16F10 melanoma cells were seeded at 1 × 104 cells/well and cultured for 48 h. Cells were treated with SFN (A) or SFN+ PF (B) in the absence or presence of α-MSH for 24 h. Melanin contents were measured at 490 nm. Arbutin was used as a melanogenesis control. The results were expressed as the mean ± SD from the three independent experiments (*p < 0.05).
Figure 2. Effect of sulforaphane, PF-3758309 or sulforaphane+ PF-3758309 on NO production. RAW 264.7 cells (1 × 104) were seeded into 24-well plates and pre-treated with SFN, PF, SFN+PF, ASA for 30 min and stimulated with or without LPS (1μg/ml) for 18 h. Cells were then treated with SFN (A), PF (B) or SFN+PF (C). The culture media was collected and subjected to Griess assay for measurement of NO generated. The results were expressed as the mean ± SD from the three independent experiments (*p < 0.05).
Table 1. EPA Dermal Classification System
Table 2. Skin Safety Evaluation Using Patch Test
Table 3. Statistical Results of Skin Brightness (V)
Table 4. The MI of Skin Pigmentation
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