Fig. 1. Method of preparing extracts for transfer ratio surveys.
Fig. 2. Standard curve of benzo(a)pyrene for the analysis by HPLC.
Fig. 3. Chromatograms of Benzo(a)pyrene: A. Benzo(a)pyrene standard of 5 ng/mL: B. Benzo(a)pyrene negative sample; C. Benzo (a)pyrene 50 ng/mL-spiked negative sample.
Fig. 4. Benzo(a)pyrene concentration of 10 samples, from each which three specimen of 5 g were randomly collected, out of each species of medicinal herbs in this study.
Table 1. Medicinal herbs used in this study
Table 2. Chemical structure and properties of Benzo(a)pyrene and 3-Methylcholanthrene
Table 3. Results of LOD, LOQ, Linearity and Recovery by HPLC-FLD
Table 4. Benzo(a)pyrene concentration in medicinal herbs in this study
Table 5. Transferred amounts of benzopyrene in decoction, soft extract, remnant after boiling for the samples of the highest and 2nd highest benzo(a)pyrene concentration, in triplicates
Table 6. Transfer ratiosa of benzopyrene in Decoction, Soft Extract, Remnant after Boiling
References
- Park, H. M., Choi, K. H., Jung, J. Y. and Lee, S. D., "Metal Exposure Through Consumption of Herbal Medicine, and Estimation of Health Risk Among Korean Population," Kor. J. Env. Health, 32(2), 186-191(2006).
- Park, M. K., Kim, S. Y. and Hwang, H. U., "A Study on the Heavy Metal Contents in Herbal Medicines: Cultivated Herbal Medicines at North Gyeongbuk Area," J. Environ. Sci., 13(2), 1117-1122(2004).
- Jo, H. Y., Jeon, J. S., Kim, B. H., Cho, S. H., Park, S. H., Kim, Y. S. and Yoon, M. H., "Safety Assessment of Liquid-Type Herbal Preparations," J. Food Hyg. Saf., 26(3), 266-272(2011).
- http://www.kpta.or.kr/information/import01.asp.
- Korean pharmacopoeia, 11th edition, Notification No. 2017-63, Ministry of Food and Drug Safety, Korea(2017).
- Kim, J. S., Lee, N. H., Lee, Y. S. and Yun, S., "Pre-planning study for the Risk Assessment of Herbal Medicines," Korea Institute for Health and Social Affairs, Korea(2014).
- Mumtaz, M. and George, J., "Toxicological Profile for Polycyclic Aromatic Hydrocarbons (PAHs)," Agency for Toxic Substances and Disease Registry, U. S. Department of health and human services (1995).
- Arey, J. and Beland, F. A. in J. Mitchell(Ed.). Some Non-heterocyclic polycyclic aromatic hydrocarbons and some related exposures, IARC Monographs on the evaluation of carcinogenic risks to humans, 92, International Agency for Research on Cancer, France(2010).
- http://www.epa.gov/ttn/atw/hlthef/polycycl.html.
- Guillen, M. D., "Polycyclic aromatic compounds: extraction and determination in food," Food Additives & Contaminants, 11(6), 669-684(1994). https://doi.org/10.1080/02652039409374268
- Djinovic, J., Popovic, A. and Jira, W., "Polycyclic Aromatic Hydrocarbons (PAHs) in Different Types of Smoked Meat Products from Serbia," Meat Science, 80(2), 449-456(2008). https://doi.org/10.1016/j.meatsci.2008.01.008
- Guideline on the use of the CTD format in the preparation of a registration application for traditional herbal medicinal product, Biopharmaceuticals and Herbal Medicine Bureau of Ministry of Food and Drug Safety, Korea(2016).
- Herbal medicine benzo(a)pyrene criteria and its analysis method, Notification No. 2009-13, Ministry of Food and Drug Safety, Korea(2009).
- Herbal medicine benzo(a)pyrene criteria and its analysis method, Notification No. 2009-302, Ministry of Food and Drug Safety, Korea(2009).
- Wilsom, R. L., Chromatography, Wiley Press, New York(2011).
- Jung, I. K., Cho, D. Y. and Row, K. H., "Separation of Whey Proteins by Anion-exchange Membranes," Korean J. Chem. Eng., 20(3), 538-541(2003). https://doi.org/10.1007/BF02705562
- Park, S. M., Ko, K. Y. and Kim, I. H., "Optimization of d-lim-Onene Extraction from Tangerine Peel in Various Solvents by Using Soxhlet Extractor," Korean J. Chem. Eng., 53(6), 717-722 (2015). https://doi.org/10.9713/kcer.2015.53.6.717
- Yoon, C. W., Bok, H. S., Choi, D. K. and Row, K. H., "Optimization Condition of Astaxanthin Extract from Shrimp Waste Using Response Surface Methodology," Korean J. Chem. Eng., 50(3), 545-550(2012). https://doi.org/10.9713/kcer.2012.50.3.545
- Lee, K. J., Cho, D. Y. and Row, K. H., "Extraction and Purification of Isoflavones from Korean Soybean and Soybean Paste," Korean Chem. Eng. Res., 41(5), 612-616(2003).
- Cho, Y. H., Song, K. Y., Baek, M. K., Lee, J. W. and Lee, G. W., "Study on Extraction Condition and Analysis Methods of Benzopyrene in Black Ginseng," Yakhak Hoeji, 56(3), 145-151(2012).
- Hu, S. J., Jin, S. H. and Choi, D. M., "Analysis of Benzo(a) Pyrene in Red Ginseng Beverage," J. Food Hyg. Saf., 23(1), 26-30(2008).
- Kim, Y. K., Park, W. H., Kim, E. H., Lee, C. Y., Jeong, H. J., Han, E. J., Kim, J. G., Jeong, Y. K. and Kim, J. H., "Analysis and Risk Assessment of Benzo[a]pyrene in Herbal Medicinal Pills," Report of S.I.H.E., 51, 127-135(2015).
- Kim, T., Nho, H. J., Jun, S. H., Kim, K. B. and Kim, D., "Antioxidation and Anti-atopic Dermatitis Effect of Herbal Wood Vinegar," Korean Chem. Eng. Res., 48(6), 690-694(2010).
- Park, C.-E. and Park, C.-H., "Ursolic Acid Isolated from Mume Fructus Inhibits Urease Activity of Helicobacter pylori," Korean Chem. Eng. Res., 51(5), 591-596(2013). https://doi.org/10.9713/KCER.2013.51.5.591
- Baek, W. S., Kim, K. H., Shin, S. H. and Lee, J., "The Monitoring of Benzo(a)pyrene's Content on the Herbal Medicines," Report 1475004098, Ministry of Food and Drug Safety, Korea(2009).
- Lee, S. D., Lee, Y. K., Kim, M. S., Park, S. K., Kim, Y. S. and Chae, Y. Z., "The Content and Risk Assessment of Heavy Metals in Herbal Pills," J. Food Hyg. Saf., 27(4), 375-387(2012). https://doi.org/10.13103/JFHS.2012.27.4.375
- Lee, S. D., Park, H. M., Lee, J. C. and Kook, Y. B., "Concentration of Metal in Herbal Drugs: In crude, Remnant After Boiling and Decoction of Herbal Drugs," J. Korean Oriental Med., 24(2), 59-65(2003).
- Kim, N. J., Sim. S. B., Ryu, J. W., Kim, J. W. and Hong, N. D., "Studies on Contents and Elution of Metals from Crude Drugs," Journal of Kyung Hee University, 12(2), 158-166(1996).
- Choi, J. G., "Research of Benzo(a)pyrene Analysis Method Verification and Improvement by Herbal Medicine," Report 1475005600, Ministry of Food and Drug Safety, Korea(2010).
- Kim, D. H., Park, J. Y., Kim, E. K., Cho, C. H., Kim, J. Y., Lee, J. H., Hyeon, S. Y., Kim, S. H., Lee, D. J. and Lee, Y. J. in D. S. Kim(Ed.), Handbook of analysis of benzo(a)pyrene in herbal medicine, National Institute of Food and Drug Safety Evaluation, Korea(2010).
- National standard of traditional medicinal(herbal and botanical) materials, Notification No. 2009-85, Ministry of Food and Drug Safety, Korea(2017).
- Kang, S. J., Lee, J. P., Kang, I. H., Kim, Y. H., Yoon, C, Y., Baek, J. H., Oh, S. W., Lee, H. Y., Kim, J. S., Lee, P. H., Ki, H. A. and Lee, E. P. in S. Y. Jang(Ed.), How to describe details of the preparation method of herbal medicine, Ministry of Food and Drug Safety, Korea(2009).
- Therapeutic products programme guideline (Q2B), "Validation of analytical procedures: Methodology," International conference on harmonisation of technical requirements for the registration of pharmaceuticals for human use(ICH), Ottawa, Ontario, Canada (1999).
- Fismes, J., Perrin-Ganier, C., Empereur-Biosoonnet, P. and Morel, J. L., "Soil-to-root Transfer and Translocation of Polycyclic Aromatic Hydrocarbons by Vegetables Grown on Industrial Contaminated Soils," J. Environ. Qual., 31(5), 1649-1656(2002). https://doi.org/10.2134/jeq2002.1649
- Tao, S., Cui, Y. H., Xu, F. L., Cao, J., Liu, W. X., Schmitt, G., Wang, X. J., Shen, W. R., Quing, B. P. and Sun, R., "Polycyclic Aromatic Hydrocarbons(PAHs) in Agricultural Soil and Vegetables from Tianjin," Sci. Total Environ., 320(1), 11-24(2004). https://doi.org/10.1016/S0048-9697(03)00453-4
- Khan, S., Aijun, L., Zhang, S., Hu, Q. and Zhu, Y. G., "Accumulation of Polycyclic Aromatic Hydrocarbons and Heavy Metals in Lettuce Grown in the Soils Contaminated with Long-term Wastewater Irrigation," J. Hazard. Mater., 152(2), 506-515(2008). https://doi.org/10.1016/j.jhazmat.2007.07.014
- Jo, S. A., Kim, S. J., Kim, N. H., Jung, S. J., Kim, H. S., Kim, K. S., Han, K. Y. and Chae, Y. J., "Analysis of Benzo(a)pyrene Content in Medicinal Herbs," Report of S. I. H. E., 47, 94-102(2011).
- Lee, S. R., Kim, A. K., Kim, S. D., Lee, H. K., Lee, H. J., Ryu, H. J., Lee, J. M., Yu, I. S. and Jung, K., "Monitoring and Risk Assessment of Benzo(a)pyrene Content in Medicinal Herbs," Kor. J. Pharmacogn., 48(3), 237-242(2017).
- Lee, M. Y., Jung, S. M. and Lee, G. W., "Monitoring on Benzo (a)pyrene Content in Oriental Medicine," Journal of Digital Convergence, 10(7), 201-206(2012). https://doi.org/10.14400/JDPM.2012.10.7.201
- Kim, G. Y., "A Research of the Manufacturing Process of Herbal Medicine," Report 1475007560, Ministry of Food and Drug Safety, Korea(2014).
- Ahn, T. J., Ahn, Y. S., Hur, M., Park, C. G. and Kim, Y. G., "Study on the New Process for the Control of Benzopyrene Formation in Herbal Products," Report PJ007594, Rural Development Administration, Korea(2013).