Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Protective Effects of Extract of Puerariae radix on Hepatic injury Induced by Carbon Tetrachloride In Rat.

갈근 추출물이 사염화탄소로 유발된 흰쥐의 간 손상에 대한 보호효과

  • Kim, Ok-Kyung (Department of Food Science and Nutrition, Dae Jin University)
  • 김옥경 (대진대학교 과학기술대학 식품영양학과)
  • Received : 2017.07.24
  • Accepted : 2017.08.29
  • Published : 2017.09.30
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Abstract

This study was done to investigate the protective effects of ethanol extract puerariae radix(Pr) on carbon tetrachloride($CCl_4$) intoxicated rats. Male sprague Dawley rats(200~210g)was used. experimental groups were divided into normal group, $CCl_4$-control group, and ethanol extract $CCl_4$-treated group. $CCl_4$-treated groups were injected with $CCl_4$ 0.6mg/kg.b.w(i.p). The activities of Alanine aminotransferase(ALT), Aspartate aminotransferase(AST), Alkaline phosphatase(ALP), Glutamyltranspeptidase(${gamma}$-GT), Lactate dehydrogenase(LDH) in extract pretrated group was significantly decreased(p<0.05) compared to the $CCl_4$-control group. The contents of triglyceride, cholesterol and lipid peroxide were significantly decreased(p<0.05). whereas content of HDL-choresterol was significantly increased.(p<0.05). In addition, activities of hepatic catalase(CAT), glutathione peroxidase(GSH-Px) in the extract pretreated rats were significantly decreased(p<0.05) compared to the $CCl_4$-control group. but the content of glutathione(GSH) was significantly increased(p<0.05). These results suggest that extract of puerariae radix(Pr) has hepatoprotective effect in the $CCl_4$-intoxicated rats.

Dawley 계 숫컷 흰쥐(200~210g)를 정상군, $CCl_4$-대조군, $CCl_4$-실험군으로 나누어 실험군은 갈근 에탄올 추출물을 1,000mg/kg,b.w의 용량으로 1일1회7일간 경구 투여 후 사염화탄소를 0.6mg/kg,b.w의 용량으로 복강내 주사 후 다음날 개복하여 혈청내의 Alanine aminotransferase(ALT), Aspartate aminotransferase(AST), Alkaline phosphatase(ALP), Glutamyltranspeptidase(${\gamma}$-GT), Lactate dehydro-genase(LDH)의 활성도와 중성지방, 콜레스테롤 함량을 측정한 결과 갈근 추출물 투여군에서 $CCl_4$-대조군과 비교하여 유의적인 감소(p<0.05)를 나타내었으며, HDL-콜레스테롤은 유의적인 증가(P0.05)를 나타내었다. 간조직중의 지질과산화 함량과 Catalase(CAT), Glutathione peroxiddase(GSH-Px)의 항산화 효소 활성은 유의적인 감소를 나타내었다. 반면에 간 조직중의 glutathione 함량은 유의적인 증가를 나타내었다. 이 실험 결과 갈근 에탄올 추출물은 사염화탄소 투여에 의한 간 손상을 억제하는 보호물질과 항산화 물질을 함유하고 있음을 알 수 있었다.

Keywords

References

  1. J., Neuzil, J. Gebick, and R. Stocker, Radical induced chain oxidation of proteins and its inhibition by chain breaking antioxidants. Biochem. J. 293: 601-606(1993). https://doi.org/10.1042/bj2930601
  2. D. Steinberg, S. Pathasarathly, T.E. Carew, J.C. Khoo, and J.L Witztum, Beyond cholesterol. Modifications of low density lipoproteins that increase its atherogenicity. N. Engl. J. Med. 320: 915-923(1989). https://doi.org/10.1056/NEJM198904063201407
  3. W. Gary, and D. Cynthia, Leaf The role of oxidative stress in HIV disease. free radical. Biology and Medicine. 19: 523-528(1995).
  4. A. G. Gilman, Carbon tetrachloride. The Pharmacological basis of therapeutics 7: 1635-1636(1985).
  5. J. V. ,Bruckner W. F. Mackenzie, and S. Muralidhara, Oral toxicity of carbon tetrachloride : Acute, subacute and subchronic studies in rats. Fund. Appl. Toxicology 6: 16-34(1986). https://doi.org/10.1016/0272-0590(86)90260-5
  6. T.C. Butler, Reduction of carbon tetrachloride in vivo and reduction of carbon tetrachloride and chloroform in vitro by tissues and constituents. J. Pharmacol. Exp. Ther. 134: 311-319 (1990).
  7. H. M Hassan, Free Radical Biol. Med., 5. 377-385(1988).
  8. T. Byers and G. Perry, Dietary carotenes, vitamin C and vitamin E as protective antioxidants in human cancers. Ann. Rev. Nutr., 12, 135-159(1992).
  9. S. J. Lee, Banchokangmok. Komunsa., Seoul, Vol.3, p537(1980).
  10. J.Her : Donguibokam. Namsandang, Seoul, Vol.3.(1984).
  11. K.Miura, R.Takeda. H. Nakamoto and HSsaito, The chemical and pharmacological study of Puerariae radix. J. Appl. Pharmacol., 5, 247(1971).
  12. C.Y. Zeng, Y.P Zhou, and L.L.Fan, Pharmacological studies on radix parariae Iv. Chunhwa I Hsueh Tsa Chin, 59, 479(1979).
  13. L.L.Fan, G.Y. Zeng, Y.P.Zhou, L.Y. Zang and Y. cheng, Pharmacological studies on radix parariae. Chin. Med. J., 95,145 (1982).
  14. S. Liu, J. Wang, C. Liu, G. Wen, A study on processing of the root of pueraria lobata(willd) ohwiby HPLC, Zhonggno zhong yao za zhi 23: 723(1998).
  15. W.M.Keung, B.B. Vallee, Daidzini, A potent selective inhibtor of human mitochodrial aldehyde dehydrogenase, prot. Natl Acad Sci. USA 90 : 1247(1993). https://doi.org/10.1073/pnas.90.4.1247
  16. T. Sato. A. Kawamoto, Y. Tamura, T. Fujii, Mechanism of antioxidant action of pueraria glycoside(PG)-1(an isoflavonoid) antioxidant chem. pharm. Tokyo Japan 40,721(1993).
  17. W.M. Keung, B.L Vallee, Kudzu, Root an ancient chinese source of modern antidipsotropic agents phytochemistry 47: 499(1978).
  18. T. Kurihara , M. Kikuchi, Studies on the constituents of flowers, V on the components of flower of pueraria thunbergiana benth. (2) isolation of a new isoflavoneglycoside Yakugaku jasshi 75:1283(1975).
  19. N. P. Peter,, K. H. T. Benny, H. T. Chee, The metabolism of hypoglycemic action of the semi-purified fractions of Averrhoa bilimbi in streptozotocin diabetic rats. Life Sciences. 70: 535(2001). https://doi.org/10.1016/S0024-3205(01)01423-0
  20. E. Rabbo and T.C. Terkildsen, On the enzymatic determination of blood glucose. Scandinav J. Lab. Invest. 12: 402(1967).
  21. J. D. Belcher, and J. O. Egan, A microenzymatic method to measure cholesterol and triglyceride in lipoprotein subfractions separated by density gradient ultracentrifugation from 200 microliters of plasma or serum. J. Lipid Res. 32: 359(1991).
  22. J. C. Murat, and A. Serfaty, Simple enzymatic determination of Polysaccharide (Glycogen) content of animal tissue. Clinical Chemistry 20: 1576(1974).
  23. E.S. Baginski, P.P. Foa, and B. Zak, Glucose 6-phosphatase in methods of enzymematic Analysis Vol. 2. Academic Press, New York, 876(1983)
  24. V. C. Rao and H. M. Nehendale, Colchicine Antimiosis Abolishes$ CCl_4$ Autoprotection, Toxicol. Pathol., 19, 597 (1991). https://doi.org/10.1177/019262339101900405
  25. O. K.Kim, The Effects of Sedum sarmentosum Bunge Extract using Super Critical Carbon Dioxide on Lipid Metabolism, Lipid Peroxidation and Antioxidation in Carbon Tetrachloride Induced Hepatotoxicity in Rats. J. of Korean Oil Chemist's Soc. 21, 204-213 (2004).
  26. A. W. Hayes, "Principles and Methods of Toxicology", p.407 Raben Press, New York(1982).
  27. A. F. Hofmann and H. Popper, Ursodeoxycholic Acid for Primary Biliary Cirrhosis, Lancet, 2, 398(1987).
  28. J. B. Whitfield, R. E. Pounder, G. Neale, and D. W. Moss, Serum ${\gamma}$-Glutamyl Transpeptidase Activity in Liver Disease, Gut, 13, 702(1972). https://doi.org/10.1136/gut.13.9.702
  29. E. G. Han and S. Y. Cho, Effect of Codonopsis lanceolata Water Extract on the Activities of Antioxidative Enzymes in Carbon Tetrachloride Treated Rats, J. Korean Soc. Food Sci. Nutr., 26, 1181 (1997).
  30. R. O. Recknagel, E. A. Glende, and A. M. Hruszkewyez, "Chemical Mechanism in Carbon Tetrachloride Toxicity. In Free Radicals in Biology", W. A. Prayor (ed), p. 97, Academic Press, New York (1997).
  31. M. J. Burkitt and J. Duncan, Effects of Trans Resveratrol on Copper Dependent Hydroxyl Radical Formation and DNA Damage : Evidence for Hydroxyl Radical Scavening and a Novel, Glutathione Sparing Mechanism of Action, Archives of Biochem. Stry. and Biophysics 381, 253 (2000). https://doi.org/10.1006/abbi.2000.1973
  32. J. P. Prohaska, and H. E Ganther, Glutathione peroxidase activities of glutathione-s-transferase purified from rat liver. Biochem. Biophys. Res. Comm. 76: 487-492(1977).
  33. J. M. Mates,, C. Perez, and I. Castro, Antioxidant enzymes and human diseases. Clin. Biochem. 32: 595-603(1999). https://doi.org/10.1016/S0009-9120(99)00075-2
  34. A. Deisseroth and A. L. Dounce, Catalase Physical and Chemical Properties, Mechanism of Catalysis and Physiological Role, Physiol. Rev., 50, 3(1970).
  35. G. Aykac, The Effects of Chronic Ethanol Indigestion on Hepatic Lipid Peroxide, Glutathione, Glutathione Peroxidase and Glutathione Transferase in Rats, Toxicol., 35, 71(1985). $H_{2}O_{2}$. Arch. Biochem. Biophys., 210, 505-516(1981).