Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Selection of Medium Components by Plackett-Burman Design for Cell Growth of a Newly Isolated Methylobacterium sp. WJ4

  • Lee, Wangjun (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Jinwon (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Received : 2016.08.26
  • Accepted : 2016.09.09
  • Published : 2016.12.01
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Abstract

We isolated a novel methylotrophic bacterium from soil collected in Dongducheon Environment Affairs Agency. The isolate was identified as Methylobacterium sp. WJ4 based on phylogenetic analysis. Plackett-Burman design was employed for screening eight parameters of nitrate mineral salts (NMS) medium for cell growth of a newly isolated Methylobacterium sp. WJ4 with experimental validation. Trace element solution and vitamin stock were found to affect cell growth, which can be further optimized for increased cell growth. This is the first report of screening parameters of NMS medium which affect cell growth of strain belonging to the genus Methylobacterium using Plackett-Burman design.

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