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http://dx.doi.org/10.9713/kcer.2016.54.6.812

Selection of Medium Components by Plackett-Burman Design for Cell Growth of a Newly Isolated Methylobacterium sp. WJ4  

Lee, Wangjun (Department of Chemical and Biomolecular Engineering, Sogang University)
Lee, Jinwon (Department of Chemical and Biomolecular Engineering, Sogang University)
Publication Information
Korean Chemical Engineering Research / v.54, no.6, 2016 , pp. 812-816 More about this Journal
Abstract
We isolated a novel methylotrophic bacterium from soil collected in Dongducheon Environment Affairs Agency. The isolate was identified as Methylobacterium sp. WJ4 based on phylogenetic analysis. Plackett-Burman design was employed for screening eight parameters of nitrate mineral salts (NMS) medium for cell growth of a newly isolated Methylobacterium sp. WJ4 with experimental validation. Trace element solution and vitamin stock were found to affect cell growth, which can be further optimized for increased cell growth. This is the first report of screening parameters of NMS medium which affect cell growth of strain belonging to the genus Methylobacterium using Plackett-Burman design.
Keywords
Cell growth; Isolation; Methylobacterium; NMS medium; Plackett-Burman design;
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