Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Development of transgenic cassava plants expressing IbOr gene by somatic embryogenesis

체세포배발생에 의한 IbOr 유전자 형질전환 카사바 개발

  • Kim, Sun Ha (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Myoung Duck (Institute of Genetic Engineering, Hankyong National University (HNU)) ;
  • Park, Sung-Chul (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jeong, Jae Cheol (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Haeng-Soon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwak, Sang-Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 김선하 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김명덕 (국립한경대학교 유전공학연구소) ;
  • 박성철 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 정재철 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 이행순 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 곽상수 (한국생명공학연구원 식물시스템공학연구센터)
  • Received : 2015.03.09
  • Accepted : 2015.03.20
  • Published : 2015.06.30
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Abstract

Cassava (Manihot esculenta Crantz) is a useful root crop for food, animal feed and various industrial materials including biofuel. Despite of its importance as an industrial crop, the genetic engineering approaches to manipulate transgenic plant development in cassava are limited. In this study, to develop new cultivar with high level of carotenoids and enhanced tolerance to environmental stresses, sweetpotato IbOr gene involved in accumulation of carotenoids was introduced into an Indonesian IDB high-yielding cassava cultivar under the control of oxidative stress-inducible SWPA2 promoter through Agrobacterium-mediated transformation of friable embryogenic calli. The 19 transgenic lines were successfully generated on the basis of gDNA-PCR and IbOr transcript levels for further characterization in terms of carotenoid contents and environmental stresses. Therefore, IbOr transgenic cassava plants may be developed for enhanced biomass production with high levels of carotenoids on marginal lands.

카사바는 열대와 아열대지역 뿌리작물로서 중요한 식량자원일 뿐만 아니라 동물 사료, 전분, 바이오에탄올 등 다양한 산업소재로서 이용이 가능하다. 그러나 카사바의 산업적 중요성에 비해 형질전환기술을 이용한 신품종 개발은 아직까지 제한적이다. 본 연구에서는 인도네시아 IDB사가 개발한 다수확 카사바 품종을 이용하여 영양강화 및 환경스트레스에 저항성을 향상시킨 카사바를 개발하기 위하여 체세포배를 이용한 식물체 재분화시스템을 확립하였다. 카로티노이드 축적에 관련된 IbOr 유전자를 체세포배를 이용한 Agrobacterium 매개방법으로 카사바에 형질전환하였다. gDNA PCR과 RT-PCR을 통해 19개의 형질전환식물체를 성공적으로 확보하였다. 향후 카로티노이드 함량분석, 환경스트레스 내성분석 등을 통하여 IbOr 카사바 식물체의 농업적 유용성을 검정할 예정이다.

Keywords

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