The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Development of Effective Screening Method for Efficacy Test of Trunk Injection Agents Against Pine Wood Nematode, Bersaphelenchus xylophilus in Japanese Black Pine, Pinus thunbergii

곰솔(Pinus thunbergii)에서 소나무재선충(Bersaphelenchus xylophilus) 나무주사 약제의 효율적인 평가법 개발

  • Shin, Won Sik (Department of Applied Biology, School of Environmental Ecology and Tourism, Kyungpook National University) ;
  • Jung, Young Hack (Devision of Applied Life Science (BK21 program), Department of Applied Biology, Gyeongsang National University) ;
  • Lee, Sang Myeong (SM Bio Co.) ;
  • Lee, Chae Min (Korea Forest Research Institute) ;
  • Lee, Chang Joon (Gyeongsangbukdo Forest and Environment Research Institute) ;
  • Kim, Dong Soo (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Mun, Il Sung (Korea Forest Research Institute) ;
  • Lee, Dong Woon (Department of Applied Biology, School of Environmental Ecology and Tourism, Kyungpook National University)
  • 신원식 (경북대학교 생물응용학과, 생태환경관광학부) ;
  • 정영학 (경상대학교 대학원 응용생명과학부(BK21)) ;
  • 이상명 ((주)SM바이오) ;
  • 이채민 (국립산림과학원) ;
  • 이창준 (경상북도 산림환경연구원) ;
  • 김동수 (국립산림과학원 남부산림자원연구소) ;
  • 문일성 (국립산림과학원) ;
  • 이동운 (경북대학교 생물응용학과, 생태환경관광학부)
  • Received : 2015.11.30
  • Accepted : 2015.12.14
  • Published : 2015.12.31
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Abstract

Pine wood nematode (PWN), Bursaphelenchus xylophilus is one of the most serious pests of pine tree. Trunk injection of some nematicides in tree is well known as an effective control method. However there are some limitating factors which hindering the efficacy of trunk injection in field or potted tree. In this study we suggested easy and useful alternative screening methods of nematicides against PWN. Reproduction of PWN was influenced by tree twig moisture (high reproduction in high moisture trig) and paraffin coating was 78.6% reduced moisture loss in tested twig. There were no reproduction different in up and down site from infection site of twig at 1 month after inoculation of PWN and also distance (5 and 10 cm) from inoculation site of PWN did not influence the reproduction of PWN. Numbers of reproduced PWN were higher with decreasing diameter of twig. Numbers of reproduced PWN were similar to P. thunbergii and P. densiflora. However reproduction was increased depending on high inoculation density and longer propagation period. When inoculation of PWN on cut twig injected with emamectin benzoate 2.15% EC and morantel tartrate 8% SL in trunk of Pinus thunbergii in the field, PWN number were significantly reduced than untreated control. We suggest this screening method for PWN control agents.

소나무재선충(Bursaphelenchus xylophilus) 소나무류의 가장 치명적인 해충의 하나로 우리나라를 비롯하여 중국, 일본, 스페인, 포르투갈 등지에서 발생하고 있다. 살선충제 나무주사는 효과적인 방제법의 하나이나 야외나 실내실험의 많은 제약이 있다. 본 연구에서는 이러한 한계점을 극복하기 위한 대체 효과 평가법을 제시하기 위하여 수행하였다. 곰솔의 가지에서 소나무재선충의 증식은 수분 손실이 적은 가지에서 증식이 많이 되었으며 파라핀 도포는 수분손실을 78.6% 감소시켰다. 소나무재선충 접종 한 달 후 자른 가지에서 소나무재선충의 증식은 접종 부위로부터 상하간에 차이가 없었으며 5cm구간과 10cm 구간 사이에도 차이가 없었다. 접종 나무의 굵기가 작을수록 재선충의 증식량이 많았으며 접종 동일 면의 증식량이 많았다. 곰솔과 소나무에서 재선충의 증식은 수종간에는 차이가 없었으며 접종밀도가 높을수록, 증식기간이 길수록 증식이 많이 되었다. Emamectin benzoate 2.15% EC와 morantel tartrate 8% SL을 곰솔에 수간주입 후 가지를 절단하여 소나무재선충을 접종하여 증식량을 조사한 결과 60일 후에 두 약제 모두 재선충의 증식을 유의하게 감소시켰다. 따라서 수간주사 한 소나무재선충의 효과를 검정하기 위하여 수간주사 한 나무의 가지에 소나무재선충을 접종하여 효과를 검정하는 방법을 제시한다.

Keywords

References

  1. Cheng, H. R., M. Lin, W. Li and Z. Fang (1983) The occurrence of a pine wilting disease caused by a nematode found in Nanjing. Forest Pest and Disease 4:1-5.
  2. Cho, I. H. (2006) Statistical consulting and SAS lecture. Youngjin.com. 805pp.
  3. Dwinell, L. D. (1997) The pinewood nematode: regulation and mitigation. Ann. Rev. Phytopathol. 35:153-166. https://doi.org/10.1146/annurev.phyto.35.1.153
  4. Dwinell, L. D. and W. R. Nickle (1989) An overview of the pinewood nematode ban in North America. General Technical Report SE-55, North America Forestry Commission Publication No. 2, Southeastern Forest Experimental Station, Forest Service, United States Department of Agriculture.
  5. Elbadri, G. A. A., D. W. Lee, J. C. Park, H. B. Yu, H. Y. Choo, S. M. Lee and T. H. Lim (2008) Nematicidal screening of essential oils and herbal extracts against Bursaphelenchus xylophilus. Plant Pathol. J. 24:178-182. https://doi.org/10.5423/PPJ.2008.24.2.178
  6. Fukuda, K., T. Hogetsu and K. Suzuki (1992) Phytosynthesis and water status of pine-wood nematode-infected pine seedlings. J. Jpn. For. Soc. 74:1-8.
  7. Futai, K. (2008) Pine wilt in Japan: from first incidence to the present. In Pine wilt disease: Zhao, B. G., Eds; Springer, Japan. pp.5-25.
  8. Ikeda, T. and T. Suzuki (1984) Influence of pine-wood nematodes on hydraulic conductivity and water status in Pinus thunbergii. J. Jap. For. Soc. 66:412-120.
  9. Iwahori, H. and K. Futai (1996) Changes in nematode population and movement in pine seedlings with the development of pine wilt disease: suppressive effects of water extracts from infected pine seedlings on the movement of nematodes. Appl. Entomol. Zool. 31:1-20. https://doi.org/10.1303/aez.31.1
  10. Kamata, N. (2008) Intergrated pest management of pine wilt disease in Japan: tactics and strategies. In Pine wilt disease: Zhao, B. G., Eds; Springer, Japan. pp.304-322.
  11. Kim, D. S., S. M. Lee, C. S. Kim, D. W. Lee and C. G. Park (2011) Movement of Monochamus alternatus Hope (Coleoptera: Cerambycidae) adults among young black pine trees in a screen cage. Korean J. Appl. Entomol. 50:1-6. https://doi.org/10.5656/KSAE.2010.04.0.014
  12. Kim, D. S., S. M. Lee, H. S. Huh, N. C. Park and C. G. Park (2009) Escape of pine wood nematode, Bursaphelenchus xylophilus, through feeding and oviposition behavior of Monochamus alternatus and M. saltuarius (Coleoptera: Cerambycidae) adult. Korean J. Appl. Entomol. 48:527-533. https://doi.org/10.5656/KSAE.2009.48.4.527
  13. Kishi, Y. (1995) The pinewood nematode and Japanese pine sawyer. Thomas, Tokyo, p302.
  14. Kishi, Y. (1999) Influence of tree age on wilt and mortality of pines after inoculation with Bursaphelenchus xylophilus. J. Jpn. For. Soc. 81:330-333.
  15. Kobayashi, F., A. Yamane and T. Ikeda (1984) The Japanese pine sawyer beetle as the vector of pine wilt disease. Ann. Rev. Entomol. 29:115-135. https://doi.org/10.1146/annurev.en.29.010184.000555
  16. Kong, J. O., S. M. Lee, Y. S. Moon, S. G. Lee and Y. J. Ahn (2006) Nematicidal activity of plant essential oils against Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). J. Asia-Pacific Entomol. 9:173-178. https://doi.org/10.1016/S1226-8615(08)60289-7
  17. Kong, J. O., S. M. Lee, Y. S. Moon, S. G. Lee and Y. J. Ahn (2007) Nematicidal activity of cassia and cinnamon oil compounds and related compounds toward Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae). J. Nematol. 39:31-36.
  18. Korea Crop Protection Association (2011) Guide book of pesticides using. Samjung Press. Seoul, Korea.
  19. Korea Forest Research Institute (KFRI) (2006) Technique guide of forest disease and insect pests. KFRI. 300pp.
  20. Korea Forest Service (2005) Practical manuals for effective control of pine wood nematode. 228pp. (In Korean)
  21. Korea Forest Service (2009) Statistical yearbook of forestry 2008. Korea Forest Service. http://www.forest.go.kr
  22. Kuroda, K., T. Yoshihiro and S. Ito (1991) Development of the pine-wilt disease in Pinus densiflora from the stand point of water conduction. J. Jpn. For. Soc. 73:69-72.
  23. Lee, D. W., S. M. Lee, S. H. Lee and H. Y. Choo (2010) Nematicidal activity of some herval extracts against the pine wood nematode, Bursaphelenchus xylophilus. Korean J. Soil Zool. 14(1-2):43-49.
  24. Lee, S. M., D. S. Kim, C. S. Kim, H. Y. Choo and D. W. Lee (2008) Possibility of simultaneous control of pine wilt disease and Thecodiplosis japonensis and or Matsucoccus thunbergianae on black pine (Pinus thunbergii) by abamectin and emamectin benzoate. Korean J. Pesticides Sci. 12:363-367.
  25. Lee, S. M., D. S. Kim, S. G. Lee, N. C. Park and D. W. Lee (2009) Selection of trunk injection pesticides for preventive of pine wilt disease by Bursaphelenchus xylophilus on Japanese black pine (Pinus thunbergii). Korean J. Pesticides Sci. 13:267-274.
  26. Mackeen, M. M., A. M. Ali, M. A. Abdullah, R. M. Nasir, N. B. Mat, A. R. Razak and K. Kawazu (1997) Antinematicidal activity of some Malaysian plant extracts against the pine wood nematode, Bursaphelenchus xylophilus. Pestic. Sci. 51:165-170. https://doi.org/10.1002/(SICI)1096-9063(199710)51:2<165::AID-PS623>3.0.CO;2-2
  27. Mamiya, Y. (1975) The life history of the pine wood nematode, Bursaphelenchus lignicolus. Jpn. J. Nematol. 5:16-25.
  28. Mamiya, Y. (1983) The effect of wood tissues on the molting rate of the dauerlarvae of Bursaphelenchus xylophilus. Japanese J. Nematol. 13:6-13.
  29. Mamiya, Y. (1988) History of pine wilt disease in Japan. J. Nematol. 20: 219-226.
  30. Maehara, N. and K. Futai (1996) Factors affecting both the numbers of pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae), carried by the Japanese pine sawyer, Monochamus alternatus (Coleoptera: Cerambycidae), and the nematode's life history. Appl. Entomol. Zool. 31:443-452. https://doi.org/10.1303/aez.31.443
  31. Maehara, N. and K. Futai (2002) Factors affecting the number of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) carried by several species of beetles. Nematology 4:653-658. https://doi.org/10.1163/15685410260438944
  32. Mota, M., H. Braasch, M. A. Bravo, A. C. Penas, W. Burgermeister, K. Metge and E. Sousa (1999) First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1:727-734. https://doi.org/10.1163/156854199508757
  33. Oku, H., T. Shiraishi and K. Chikamatsu (1989) Active defense as a mechanism of resistance in pine against pine wilt disease. Ann. Phytopath. Soc. Japan. 55:603-608. https://doi.org/10.3186/jjphytopath.55.603
  34. Rutherford, T. A., Y. Mamiya and J. M. Webster (1990) Nematode-induced pine wilt disease: factors influencing its occurrence and distribution. Forest Science 36:145-155.
  35. Shin, S. C. (2008) Pine wilt in Korea. In Pine wilt disease: Zhao, B. G., Eds; Springer, Japan. pp.26-32.
  36. Takai, K., T. Soejima, T. Suzuki and K. Kawazu (2000) Emamectin benzoate as a candidate for a trunk-injection agent against the pine wood nematode, Bursaphelenchus xylophilus. Pest Manag. Sci. 56:937-941. https://doi.org/10.1002/1526-4998(200010)56:10<937::AID-PS213>3.0.CO;2-B
  37. Takai, K., T. Soejima, T. Suzuki and K. Kawazu (2001) Development of a water-soluble preparation of emamectin benzoate and its preventive effect against the wilting of potgrown pine trees inoculated with the pine wood nematode, Bursaphelenchus xylophilus. Pest Manag. Sci. 57:463-466. https://doi.org/10.1002/ps.301
  38. Takai K., T. Suzuki and K. Kawazu (2003a) Development and preventative effect against pine wilt disease of a novel liquid formulation of emamectin benzoate. Pest Manag Sci. 59:365-370. https://doi.org/10.1002/ps.651
  39. Takai K., T. Suzuki and K. Kawazu (2003b) Distribution and persistence of emamectin benzoate at efficacious concentrations in pine tissues after injection of a liquid formulation. Pest Manag. Sci. 60:42-48.
  40. Takemoto, S. (2008) Population ecology of Bursaphelenchus xylophilus. . In Pine wilt disease: Zhao, B. G., Eds; Springer, Japan. pp.105-122.
  41. Takeuchi, Y. (2008) Host fate following infection by the pine wood nematode. In Pine wilt disease: Zhao, B. G., Eds; Springer, Japan. pp.235-249.
  42. Tomlin, C. D. S. (2006) A world compendium the pesticide manual. BCPC.Hampshire, UK.
  43. Tzean, S. and S. Jan (1985) The occurrence of pinewood nematode, Bursaphelenchus xylophilus in Taiwan. pp.38-39, In Proceedings of the 6th ROC symposium of electron microscopy.
  44. Yano, M. (1913) Investigation on the causes of pine mortality in Nagasaki prefecture. Sanrinkoho 4:1-14.
  45. Yi, C. K., B. H. Byun, J. D. Park, S. I. Yang and K. H. Chang (1989) First finding of the pinewood nematode, Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle and its insect vector in Korea. Res. Rep. For. Res. Inst. 38:141-149.

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