• Title/Summary/Keyword: 살선충제

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Effect of Nematicide-dipping Methods for the Control of Aphelenchoides fragariae in Strawberry (살선충제 침지처리에 의한 딸기잎선충 방제)

  • Kim, Dong-Geun;Kang, Myeong-Won;Lee, Joong-Hwan
    • Korean journal of applied entomology
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    • v.47 no.1
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    • pp.101-105
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    • 2008
  • Effects of different application of nematicides (fosthiazate 5% G, ethoprophos 5% G, and diazinon 34% EC) for the control of Aphelenchoides fragariae in strawberry were evaluated in a greenhouse experiments. Mother strawberry (Fragaria grandiflora) cv. Yeohong were dipped in solution of nematicides (fosthiazate or ethoprophos at 2.5 g a.i./liter in $20^{\circ}C\;or\;46^{\circ}C$) for 10 min. and planted in a greenhouse for dipping treatment. For the compare, mother strawberry were dipped in hot water for 10 min. without chemicals. For soil treatment, fosthiazate or ethoprophos at 3 kg a.i./ha were mixed into soil. For foliar spray, diazinon at 3.4 g a.i./liter was sprayed at foliage until runoff. At 40, 80, and 100 days after planting, runners were harvested from each treatment and the rate of nematode infestation and the number of nematodes per plant were examined. After 100 days of planting, mother strawberry plants dipped in fosthiazate solution (2.5 g a.i./liter, $20^{\circ}C$) for 10 min. produced more number of healthy runners and reduced % of infected runner as much as 90% and also had fewer nematodes per runner. Fosthiazate was more effective than ethoprophos. Foliar application of diazinon was reduced A. fragariae populations only in early season. Hot water treatment and nematicide soil treatment were less effective.

Effects of Treatment Time of Cadusafos and Fosthiazate for the Control of Meloidogyne arenaria on Oriental Melon (카두사포스와 포스치아제이트 처리시기에 따른 땅콩뿌리혹선충 방제효과)

  • 김동근;김진배;이재국;최성국;윤재탁
    • Korean journal of applied entomology
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    • v.41 no.4
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    • pp.293-298
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    • 2002
  • Cadusafos and fosthiazate were applied at the rate of 1.8 g a.i./6$\m^2$ as pre-plant or pre-plant+post-plant treatments on winter-grown oriental melon in a greenhouse soil infested with Meloidogyne arenaria. Nematicides reduced root-knot nematode population from 35 to 90% compared with control; fosthiazate was better than cadusafos (P = 0.003) and fosthiazate pre-plant+post-plant application reduced nematode population densities as much as 90%. Nematicides increased yield in an average of 23% (11-38%) in May, 39% (2-65%) in June, and 31% (12-46%) for the total (P = 0.085). The residue in the fruit of oriental melon by post-plant treatment of fosthiazate exceeded maximum residue limit of 0.2ppm, while the Cadusafos residue was below the limit. It is concluded that fosthiazate cannot be used as a post-plant treatment in winer-grown oriental melon with overall consideration, i. e., danger of residue, value of melon, costs of nematicide, consumer demand on safer agricultural products, and effects of fallow.

Effects of Incorporation Method of Nematicides on Reproduction of Meloidogyne arenaria (살선충제의 처리방법에 따른 뿌리혹선충 방제효과)

  • 김동근;최성국
    • Korean journal of applied entomology
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    • v.40 no.1
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    • pp.89-95
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    • 2001
  • Effects of application method of eight nematicides on reproduction of iweloidogyne arenaria were tested in pot. In pre-treatment, cadusafos , dazomet, fosthiazate reduced over 90% in number of egg masses of M. arenaria at recommended rate . Carbofuran showed similar reduction only at three times of recommended rate. Benfuracarb was the least effective. Post-treatments, in general, were less effective than pre-treatment except fostiazate which reduced root egg mass of iW. arenaria as much as pre-treatment at recommended rate . Systematic nematicide, fosthiazate has potential as post-treatment nematicide.

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Evaluation of Two Nematicides in the Initial Population Changes of the Soybean Cyst Nematode (콩시스트 선충의 초기밀도 변화에 있어서 두가지 살선충제의 효과검정)

  • ;Robert D. Riggs
    • Korean journal of applied entomology
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    • v.27 no.1
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    • pp.35-40
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    • 1988
  • The control efficiency of the nematicides, aldicarb and carbofuran, in the population dynamics of the soybean cyst nematode(Heterodera glycines)was evaluated. Nematode viability, penetration and reproduction were examined in greenhouse experiments. Nematode viability(movement) was greatly reduced by aldicard, but mot by carbofuran. Penetration was inhibited by both chemicals. Percentages of Pentration relative to the untreated check 3 days after treatment were 0.4% and 1.7% for aldicarb and carbofuran, respectively. Systemic effect ot the nematicides were observed with both nematicides but effect was greater with aldicarb than with carbofuran. The total effect of the nematicides, applied ay the time of planting soybean in infested soil, appeared to be less than the sum of the contact and systemic effects on the initial population dynamics of H. glycines.

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Purification Nematicidal Substance and Nematicidal Activity from Ginkgo biloba L. Outer Seedcoat (은행 외종피로부터 살선충 물질의 순수 분리와 활성)

  • Jang, Yu Ju;Hwang, Hyeon Jeong;Kim, Keun Ki
    • Korean Journal of Organic Agriculture
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    • v.29 no.1
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    • pp.97-109
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    • 2021
  • Plant parasitic nematodes are causing significant damage in crop production. There is a need to develop eco-friendly nematicide that reduces the damage of nematode and has little effect on the environment and human. In this study, we have isolated a substance having nematicidal activity from Ginkgo biloba L. outer seedcoat. Studies of G. biloba L. outer seedcoat are insufficient compared to the seed and leaves due to their odor and toxicity. The dried G. biloba L. outer seedcoat was extracted with dichloromethane:methanol (1:1) and fractionated into hexane, ethyl acetate and H2O. Four steps TLC were performed from EtOAc fraction to purely isolate GB4-3 with nematicidal activity. To compare nematicidal activity, G. biloba L. seedcoat methanol extract and purified GB4-3 were investigated in terms of treatment concentration and time. As a result, the nematicidal activity increased with concentration and time. In the place treated with 20 ㎍/mL of crude G. biloba L. seedcoat MeOH extract, strong activity appeared after 12 hours, and 46% nematicidal activity shown after 18 hours. About 69% of nematicidal activity was confirmed in the place where GB4-3 purified from outer seedcoat was treated with 20 ㎍/mL, and the possibility of development as nematicide was very high. This study could be used as a basic data for the development of a nematode preparation from G. biloba L. outer seedcoat.

Nematicidal Efficacy of Herbal Extracts against Meloidogyne hapla (당근뿌리혹선충에 대한 식물추출물의 살선충 효과)

  • Lee, Jung-Su;Choo, Ho-Yul;Lee, Dong-Woon
    • Korean journal of applied entomology
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    • v.50 no.4
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    • pp.315-324
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    • 2011
  • The nematicidal and egg haching inhibitory effects of extracts from 30 herbal plants (total 32 samples) against Meloidogyne hapla J2 juveniles and eggs was tested using the dipping method. At 1,000 ppm, extracts of Daphne genkwa flower buds, Eugenia caryophyllata flowers, Quisqualis indica fruits, and Zingiber officinale rhizomes produced > 80% mortality in J2 juveniles. At 125 ppm, extracts of D. genkwa and Q. indica produced 91 and 99% mortality, respectively. The toxicity of 5 selected plant extracts to M. hapla differed depending on the solvent used (i.e. hexane, methanol, hot water, or cold water). Hot water extracts of Z. officinale and Q. indica produced nematicidal efficacies of 99 and 99%, compared to 36 and 98%, respectively, with cold water extraction. Q. indica extract was highly active against M. hapla regardless of extraction method. The inhibitory effects of Areca catechu, D. genkwa, Desmodium caudatum, Pharbitis nil, Q. indica, and Z. officinale extracts on egg hatching of M. hapla was evaluated. At 1,000 ppm, D. genkwa, P. nil, and Q. indica extracts significantly reduced hatching at 7, 14 and 21 days after treatment. Numbers of juveniles in soil treated with the methanol extract D. genkwa (1,000 ppm) were significantly lower than in untreated soil in trials in pots and in a ginseng (Phanax ginseng) field. These results indicate that Q. indica extracts could be used as an environmental friendly control agent of M. hapla.

Isolation and Identification of Micromonospora sp. Showing Nematocidal Activity Against Pine Wood Nematode (소나무재선충에 대한 살선충 활성을 나타내는 Micromonospora sp.의 분리 및 동정)

  • Park, Dong-Jin;Lee, Jae-Chan;Kim, Pan-Kyung;Kim, Chang-Jin
    • The Korean Journal of Pesticide Science
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    • v.12 no.1
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    • pp.97-101
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    • 2008
  • For the isolation of Actinomycetes showing nematocidal activity against Pine wood nematode, Bursaphelenchus xylophilus, about 2000 culture broth of Actinomycetes were tested and their activity were compared with that of Streptomyces avermitilis resulting a selected strain AW050027. The cultural, morphological and physiological analysis was performed for the identification of a selected strain. Phylogenetic analyses based on 165 rDNA gene sequences showed that the selected strain AW050027 belonged to the genus Micromonospora and M. corioriae $NAR01^T$ was the closest neighbors, sharing 98.9% 165 rDNA gene sequence similarity.

Control Effects of Imicyafos GR against Two Species of the Root-knot Nematodes (Meloidogyne incognita and Meloidogyne hapla) (살선충제 Imicyafos 입제의 2종 뿌리혹선충에 대한 방제 효과)

  • Kim, Hyeong Hwan;Jung, Young Hak;Kim, Dong Hwan;Ha, Tae Ki;Yoon, Jung Beom;Park, Chung Gyoo;Choo, Ho Yul
    • The Korean Journal of Pesticide Science
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    • v.19 no.2
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    • pp.101-105
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    • 2015
  • Efficacy of novel nematicide, imicyafos GR was evaluated against two species of the root-knot nematodes in pot and greenhouse conditions. When tested in pots, the population of Meloidogyne incognita and M. hapla was reduced sixty days after treatment, with mortality rate of 91.5% and 90.6%, respectively. Suppression effect of imicyafos GR on root galling in tomato was tested. The number of root galls caused by M. incognita and M. hapla was reduced 60 days after nematode inoculation, with the efficacy of 94.2% and 95.1%, respectively. Under greenhouse conditions planted with watermelon, melon, cucumber, and tomato, the efficacy of imicyafos GR on M. incognita persisted up to 60 days after treatment, showing 90% of control efficacy. Moreover, the number of root galling was more reduced than fosthiazate treatment, with the potential as a control agent.

Comparative Responses of Sugar Beet Cyst Nematode, Heterodera schachtii and Clover Cyst Nematode, H. trifolii to Different Nematicides (클로버씨스트선충과 사탕무씨스트선충 알과 유충에 대한 약제 반응 비교)

  • Jeong, Mungi;Lee, Dong Woon
    • Korean journal of applied entomology
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    • v.59 no.4
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    • pp.361-369
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    • 2020
  • This study aimed to investigate the differences in the effects of nematicidal materials (Burkholderia rinojensis, fluazaindolizine, fluopyram, fosthiazate, hydrogen peroxide, and imicyafos) on eggs and larvae of the sugar beet cyst nematode, Heterodera schachtii and clover cyst nematode, H. trifolii, which have recently become problematic in highland Chinese cabbage cultivation areas in Korea. Comparison of the hatching rates according to the test materials showed differences between treatments. In the treatment with fluopyram, fosthiazate, and imicyafos, a very high hatching inhibitory effect of 99% was observed in both nematodes, and there was no difference in the efficacy of treatments between nematode species except for that with hydrogen peroxide. Regarding the nematocidal activity of treatments against hatched larvae, only the fluazaindolizine treatment, despite showing a high hatching inhibitory effect, showed a low nematicidal effect of less than 30% against both nematodes, whereas a mortality rate of more than 90% was observed in the other treatments. There was no difference between nematode species in the reactions of the 2nd instar juveniles to all nematicidal test materials after 24 and 48 h of treatment. Although there are limitations to the laboratory experiment, the reaction to the nematicidal materials of the two cyst nematodes that damage the same host was similar, and simultaneous control is suggested in the areas where the two nematodes occur together.

Selection and Characterization of Bacillus licheniformis MH48 for the Biocontrol of Pine Wood Nematode (Bursaphelenchus xylophilus) (소나무재선충 생물학적 방제를 위한 Bacillus licheniformis MH48의 선발 및 특성 규명)

  • Jeong, Min-Hae;Yang, Seo-Young;Lee, Yong-Sung;Ahn, Young-Sang;Park, Yun-Serk;Han, Hye-rim;Kim, Kil-Yong
    • Journal of Korean Society of Forest Science
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    • v.104 no.3
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    • pp.512-518
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    • 2015
  • Pine wilt disease (PWD) caused by pine wood nematode, Bursaphelenchus xylophilus, has become the most serious threat to pine trees in Korea. This study was subjected to investigate effective biological control agent against PWD. To select nematocidal bacteria against PWD, Bacillus licheniformis MH48 was selected among five bacteria due to its high nematocidal potential. B. licheniformis MH48 was tested for cell growth and protease activity to evaluate its nematicidal potential. In the B. licheniformis MH48, cell numbers were highest three days after incubation, while protease activity was highest after seven days. In the effect of different concentrations of B. licheniformis MH48 culture broth against B. xylophilus, 20% concentration of culture broth showed approximately 80% of pine wood nematode mortality compared to the control. Especially, pine wood nematode's cuticle layers were degraded two days after treatment of B. licheniformis MH48 culture broth. The present study suggests that B. licheniformis MH48 can be one of the potential biocontrol candidates against pine wood nematode due to its ability to produce protease.